(Q42603062)

3 November 2017

title

Open sandwich ELISA with V(H)-/V(L)-alkaline phosphatase fusion proteins (English)

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3 November 2017

Suzuki C

1

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3 November 2017

Ueda H

2

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3 November 2017

Tsumoto K

3

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3 November 2017

Mahoney WC

4

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3 November 2017

Kumagai I

5

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3 November 2017

Nagamune T

6

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3 November 2017

language of work or name

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1 April 1999

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Journal of Immunological Methods

3 November 2017

published in

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3 November 2017

volume

224

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3 November 2017

issue

1-2

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3 November 2017

page(s)

171-184

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Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection.

3 November 2017

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3-D structure of a mutant (Asp101-->Ser) of E.coli alkaline phosphatase with higher catalytic activity

7 January 2021

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Recombinant colorimetric antibodies: construction and characterization of a bifunctional F(ab)2/alkaline phosphatase conjugate produced in Escherichia coli.

7 January 2021

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7 January 2021

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Antigen detection using recombinant, bifunctional single-chain Fv fusion proteins synthesised in Escherichia coli.

7 January 2021

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A comparison of strategies to stabilize immunoglobulin Fv-fragments

7 January 2021

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Magnesium in the active site of Escherichia coli alkaline phosphatase is important for both structural stabilization and catalysis.

7 January 2021

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Equilibrium and kinetic aspects of the interaction of isolated variable and constant domains of light chain with the Fd' fragment of immunoglobulin G

7 January 2021

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Rapid and efficient site-specific mutagenesis without phenotypic selection

7 January 2021

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Kinetics of dimerization of the Bence-Jones protein Au

7 January 2021

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Mutagenesis of conserved residues within the active site of Escherichia coli alkaline phosphatase yields enzymes with increased kcat

7 January 2021

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Enhanced catalysis by active-site mutagenesis at aspartic acid 153 in Escherichia coli alkaline phosphatase

7 January 2021

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A plasmid expression system for quantitative in vivo biotinylation of thioredoxin fusion proteins in Escherichia coli

7 January 2021

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Refined structure of alkaline phosphatase from Escherichia coli at 2.8 A resolution

7 January 2021

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Self-association of human immunoglobulin kappa I light chains: role of the third hypervariable region

7 January 2021

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Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system

7 January 2021

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Construction, bacterial expression, and characterization of hapten-specific single-chain Fv and alkaline phosphatase fusion protein.

7 January 2021

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Effect of the order of antibody variable regions on the expression of the single-chain HyHEL10 Fv fragment in E. coli and the thermodynamic analysis of its antigen-binding properties.

7 January 2021

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Novel selection method for engineered antibodies using the mechanism of Fv fragment stabilization in the presence of antigen.

7 January 2021

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Synthesis and expression of a DNA encoding the Fv domain of an anti-lysozyme monoclonal antibody, HyHEL10, in Streptomyces lividans

7 January 2021

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Open sandwich ELISA: a novel immunoassay based on the interchain interaction of antibody variable region

7 January 2021

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Application of an alkaline phosphatase fusion protein system suitable for efficient screening and production of Fab-enzyme conjugates in Escherichia coli.

7 January 2021

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Construction, bacterial expression and characterization of a bifunctional single-chain antibody-phosphatase fusion protein targeted to the human erbB-2 receptor

7 January 2021

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Making antibodies by phage display technology

7 January 2021

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10.1016/S0022-1759(99)00020-4

7 January 2021

Identifiers

DOI

1 reference

3 November 2017

1 reference