(Q43596202)

25 November 2017

title

Engineering the pH-optimum of a triglyceride lipase: from predictions based on electrostatic computations to experimental results (English)

1 reference

25 November 2017

1 reference

Neves-Petersen MT

1

1 reference

25 November 2017

Petersen EI

2

1 reference

25 November 2017

Fojan P

3

1 reference

25 November 2017

Noronha M

4

1 reference

25 November 2017

Madsen RG

5

1 reference

25 November 2017

Petersen SB

6

1 reference

25 November 2017

publication date

1 May 2001

1 reference

25 November 2017

published in

Journal of Biotechnology

1 reference

25 November 2017

volume

87

1 reference

25 November 2017

issue

3

1 reference

25 November 2017

page(s)

225-254

1 reference

27 Modification of enzyme catalysis by engineering surface charge

25 November 2017

cites work

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Comparison of protein electrostatic potential along the catalytic triad of serine proteinases

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

The blind watchmaker and rational protein engineering

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Prediction of pH-dependent properties of proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

The determinants of pKas in proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Electrostatic control of the rate-determining step of the copper, zinc superoxide dismutase catalytic reaction

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

pKa's of ionizable groups in proteins: atomic detail from a continuum electrostatic model

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Engineering enzyme specificity by "substrate-assisted catalysis"

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Engineering subtilisin BPN' for site-specific proteolysis.

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Site-directed mutagenesis of virtually any plasmid by eliminating a unique site

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Probing the altered specificity and catalytic properties of mutant subtilisin chemically modified at position S156C and S166C in the S1 pocket

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Engineering surface charges in a subtilisin

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

The role of lysine-234 in beta-lactamase catalysis probed by site-directed mutagenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Can a simple function for the dielectric response model electrostatic effects in globular proteins?

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Electrostatic recognition between superoxide and copper, zinc superoxide dismutase

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

On the calculation of electrostatic interactions in proteins.

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Calculation of electrostatic potentials in an enzyme active site

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Energetics of charge-charge interactions in proteins.

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Calculation of the total electrostatic energy of a macromolecular system: solvation energies, binding energies, and conformational analysis

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Theory of electrostatic interactions in macromolecules

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Relationship between mutability, polarity and exteriority of amino acid residues in protein evolution

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Amino acid difference formula to help explain protein evolution

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Treatment of electrostatic effects in macromolecular modeling

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Classical electrostatics in biology and chemistry

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

High efficiency transformation of Escherichia coli with plasmids

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Contribution of long-range electrostatic interactions to the stabilization of the catalytic transition state of the serine protease subtilisin BPN'

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Elucidating structure-mechanism relationships in lipases: prospects for predicting and engineering catalytic properties

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Family of G protein alpha chains: amphipathic analysis and predicted structure of functional domains

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Intrinsic protein electric fields: basic non-covalent interactions and relationship to protein-induced Stark effects

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

The role of protein surface charges in ion binding

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Electrostatic effect upon association of reduced nicotinamide adenine dinucleotide and equine liver alcohol dehydrogenase

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Long-range surface charge-charge interactions in proteins. Comparison of experimental results with calculations from a theoretical method.

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Engineering a thermostable protein via optimization of charge-charge interactions on the protein surface

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Atomic resolution (1.0 A) crystal structure of Fusarium solani cutinase: stereochemical analysis

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Protein engineering of chymosin; modification of the optimum pH of enzyme catalysis

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

pH Dependence of binding reactions from free energy simulations and macroscopic continuum electrostatic calculations: Application to 2′GMP/3′GMP binding to ribonuclease T1 and implications for catalysis

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Cutinase, a lipolytic enzyme with a preformed oxyanion hole

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Anion binding and pH-dependent electrostatic effects in ribonuclease

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Electrostatic effects in proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

pH-dependent processes in proteins.

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Stereochemical quality of protein structure coordinates

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Engineering of human lysozyme as a polyelectrolyte by the alteration of molecular surface charge.

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Protein folding and association: insights from the interfacial and thermodynamic properties of hydrocarbons

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Contribution of cutinase serine 42 side chain to the stabilization of the oxyanion transition state

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Electrostatics in the active site of an alpha-amylase

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Surface and electrostatics of cutinases

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Protein engineering the surface of enzymes

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

How do lipases and esterases work: the electrostatic contribution

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Characterization of the electrostatic perturbation of a catalytic site (Cys)-S-/(His)-Im+H ion-pair in one type of serine proteinase architecture by kinetic and computational studies on chemically mutated subtilisin variants.

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Surface charge measurements on Micrococcus lysodeikticus and the catalytic implications for lysozyme.

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

The modelling of electrostatic interactions in the function of globular proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Rational modification of enzyme catalysis by engineering surface charge

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Electrostatic effects on modification of charged groups in the active site cleft of subtilisin by protein engineering

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

DNA sequencing with chain-terminating inhibitors

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

A precise analytical method for calculating the electrostatic energy of macromolecules in aqueous solution

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Lipases: Interfacial Enzymes with Attractive Applications

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Electrostatic interactions in macromolecules: theory and applications

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Electrical potentials in trypsin isozymes

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Prediction of electrostatic effects of engineering of protein charges.

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Use of T7 RNA polymerase to direct expression of cloned genes

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Alteration of catalytic properties of chymosin by site-directed mutagenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Interpretation of protein titration curves. Application to lysozyme

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

An unequivocal example of cysteine proteinase activity affected by multiple electrostatic interactions

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Electrostatic interactions in proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Calculations of electrostatic properties in proteins. Analysis of contributions from induced protein dipoles

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Improving the accuracy of protein pKa calculations: conformational averaging versus the average structure.

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

1 reference

7 January 2021

Calculations of electrostatic interactions in biological systems and in solutions

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Electrostatic Energy and Macromolecular Function

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Electrostatic effects in macromolecules: fundamental concepts and practical modeling

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

The activity of porcine pancreatic phospholipase A2 in 20% alcohol/aqueous solvent, by experiment and electrostatics calculations.

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Improved continuum electrostatic modelling in proteins, with comparison to experiment.

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Simplified methods for pKa and acid pH-dependent stability estimation in proteins: removing dielectric and counterion boundaries.

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Designing substrate specificity by protein engineering of electrostatic interactions

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

On the calculation of pKas in proteins

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

Modeling of protein conformational fluctuations in pKa predictions

1 reference

https://api.crossref.org/works/10.1016%2FS0168-1656%2801%2900240-1

7 January 2021

10.1016/S0168-1656(01)00240-1

Identifiers

DOI

1 reference

25 November 2017

1 reference