(Q43786433)

26 November 2017

title

A cGMP-dependent protein kinase assay for high throughput screening based on time-resolved fluorescence resonance energy transfer (English)

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26 November 2017

B Bader

1

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26 November 2017

E Butt

2

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26 November 2017

A Palmetshofer

3

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26 November 2017

U Walter

4

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26 November 2017

T Jarchau

5

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26 November 2017

P Drueckes

6

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26 November 2017

publication date

1 August 2001

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Journal of Biomolecular Screening

26 November 2017

published in

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26 November 2017

volume

6

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26 November 2017

issue

4

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26 November 2017

page(s)

255-264

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Distinct and specific functions of cGMP-dependent protein kinases

26 November 2017

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Defective smooth muscle regulation in cGMP kinase I-deficient mice

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Increased adhesion and aggregation of platelets lacking cyclic guanosine 3',5'-monophosphate kinase I.

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Testing for endothelial dysfunction

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Cyclic GMP phosphodiesterase-5: target of sildenafil

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Regulation of cAMP and cGMP signaling: new phosphodiesterases and new functions

21 January 2018

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Smooth muscle cell expression of type I cyclic GMP-dependent protein kinase is suppressed by continuous exposure to nitrovasodilators, theophylline, cyclic GMP, and cyclic AMP

21 January 2018

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High-throughput screening: new technology for the 21st century

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Homogeneous proximity tyrosine kinase assays: scintillation proximity assay versus homogeneous time-resolved fluorescence.

21 January 2018

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A scintillation proximity assay for the Raf/MEK/ERK kinase cascade: high-throughput screening and identification of selective enzyme inhibitors

21 January 2018

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A chemiluminescent microtiter plate assay for sensitive detection of protein kinase activity

21 January 2018

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Detection of phosphopeptides by fluorescence polarization in the presence of cationic polyamino acids: application to kinase assays

21 January 2018

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A microchip-based enzyme assay for protein kinase A.

21 January 2018

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Analysis and regulation of vasodilator-stimulated phosphoprotein serine 239 phosphorylation in vitro and in intact cells using a phosphospecific monoclonal antibody.

21 January 2018

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Expression, purification, and characterization of the cGMP-dependent protein kinases I beta and II using the baculovirus system

21 January 2018

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Endogenous expression of type II cGMP-dependent protein kinase mRNA and protein in rat intestine. Implications for cystic fibrosis transmembrane conductance regulator

21 January 2018

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21 January 2018

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21 January 2018

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Concentration and regulation of cyclic nucleotides, cyclic-nucleotide-dependent protein kinases and one of their major substrates in human platelets. Estimating the rate of cAMP-regulated and cGMP-regulated protein phosphorylation in intact cells

21 January 2018

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Homogeneous fluorescence readouts for miniaturized high-throughput screening: theory and practice

21 January 2018

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A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays

21 January 2018

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Analysis of the functional role of cGMP-dependent protein kinase in intact human platelets using a specific activator 8-para-chlorophenylthio-cGMP

21 January 2018

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(Rp)-8-pCPT-cGMPS, a novel cGMP-dependent protein kinase inhibitor.

21 January 2018

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21 January 2018

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The graphical determination of Km and Ki

21 January 2018

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Regulation of human endothelial cell focal adhesion sites and migration by cGMP-dependent protein kinase I

21 January 2018

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KT5823 inhibits cGMP-dependent protein kinase activity in vitro but not in intact human platelets and rat mesangial cells

21 January 2018

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High-throughput and ultra-high-throughput screening: solution- and cell-based approaches

21 January 2018

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10.1177/108705710100600407

21 January 2018

Identifiers

DOI

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26 November 2017

1 reference