(Q43846635)

27 November 2017

title

Selection and analysis of non-interactive mutants in the Escherichia coli tryptophan synthase alpha subunit (English)

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27 November 2017

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1

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27 November 2017

J Kuhn

2

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27 November 2017

G S Stewart

series ordinal

3

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27 November 2017

publication date

1 May 1992

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Molecular Genetics and Genomics

27 November 2017

published in

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27 November 2017

volume

233

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27 November 2017

issue

1-2

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27 November 2017

page(s)

129-135

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Evolution of a biosynthetic pathway: the tryptophan paradigm

27 November 2017

cites work

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12 December 2020

The enzymatic conversion of anthranilic acid to indole.

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12 December 2020

The tryptophan synthase bienzyme complex transfers indole between the alpha- and beta-sites via a 25-30 A long tunnel

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12 December 2020

The molecular biology of tryptophan synthase: a model for protein-protein interaction

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12 December 2020

Optimization strategies for the polymerase chain reaction

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12 December 2020

STUDIES ON THE ACTIVE SITE OF THE A PROTEIN SUBUNIT OF THE ESCHERICHIA COLI TRYPTOPHAN SYNTHETASE

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12 December 2020

Reversion of trpA nonsense mutations by deletion of the chain-termination codons.

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12 December 2020

Site-directed mutagenesis of the alpha subunit of tryptophan synthase from Salmonella typhimurium.

1 reference

12 December 2020

Selection for new amino acids at position 211 of the tryptophan synthetase alpha chain of Escherichia coli.

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12 December 2020

Affinity chromatography of tryptophan synthase from Escherichia coli. Systematic studies with immobilized tryptophanol phosphate

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12 December 2020

Tryptophan synthetase: its charmed history.

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12 December 2020

Errors in the polymerase chain reaction

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12 December 2020

HIV-1 isolates are rapidly evolving quasispecies: evidence for viral mixtures and preferred nucleotide substitutions

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12 December 2020

Association of the α and β2 Subunits of the Tryptophan Synthetase of Escherichia coli

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12 December 2020

Pressure effects on the association of the α and β2 subunits of tryptophan synthetase from Escherichia coli and Salmonella typhimurium

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12 December 2020

Selection for new codons corresponding to position 234 of the tryptophan synthetase alpha chain of Escherichia coli

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12 December 2020

A novel ochre mutation in the beta-thalassemia gene of a Thai. Identification by direct cloning of the entire beta-globin gene amplified using polymerase chain reactions

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12 December 2020

High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase

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12 December 2020

Physiological Advantage of the Mechanism of the Tryptophan Synthetase Reaction

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12 December 2020

Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase

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12 December 2020

The association of tryptophan synthetase subunits from Escherichia coli and Salmonella typhimurium in homologous and heterologous combinations

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12 December 2020

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

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12 December 2020

Rapid site-specific mutagenesis in plasmids

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12 December 2020

The Tryptophan Synthase from Escherichia coli. An Improved Purification Procedure for the alpha-Subunit and Binding Studies with Substrate Analogues

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12 December 2020

Structural and functional roles of the cysteine residues in the α subunit of the Escherichia coli tryptophan synthetase. I. Structural roles and reactivity of the cysteine residues

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12 December 2020

The quaternary structure of tryptophan synthase from Escherichia coli. Fluorescence and hydrodynamic studies

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12 December 2020

pHG276: a multiple cloning site pBR322 copy number vector expressing a functional lac alpha peptide from the bacteriophage lambda PR promoter

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12 December 2020

Prediction of secondary structure by evolutionary comparison: application to the alpha subunit of tryptophan synthase

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12 December 2020

DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA

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12 December 2020

PCR based gene engineering of the Vibrio harveyi lux operon and the Escherichia coli trp operon provides for biochemically functional native and fused gene products

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12 December 2020

A method to detect ras point mutations in small subpopulations of cells

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12 December 2020

A Steady-State Kinetic Investigation of the Reaction Mechanism of the Tryptophan Synthetase of Escherichia coli

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12 December 2020

Mutants of Escherichia coli defective in the B protein of tryptophan synthetase. I. Selection and classification

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12 December 2020

Cloning in Escherichia coli of the enterotoxin gene from Clostridium perfringens type A.

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12 December 2020

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase

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12 December 2020

The catalytic mechanism of tryptophan synthase from Escherichia coli. Kinetics of the reaction of indole with the enzyme--L-serine complexes

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12 December 2020

Tryptophan synthase from Escherichia coli and Salmonella typhimurium.

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12 December 2020

Tryptophan Synthase: Structure, Function, and Subunit Interaction

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12 December 2020

A rapid method for site-specific mutagenesis and directional subcloning by using the polymerase chain reaction to generate recombinant circles.

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12 December 2020

Streptomycin-Suppressible Lethal Mutations in Escherichia coli

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12 December 2020

Reaction of the α Subunit of the Escherichia coli Tryptophan Synthetase with 1,5-Difluoro-2,4-dinitrobenzene

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12 December 2020

A mutational alteration of the tryptophan synthetase of Escherichia coli

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12 December 2020

Structural and functional roles of the cysteine residues in the alpha subunit of the Escherichia coli tryptophan synthetase. 3. Studies with the bifunctional sulfhydryl reagent bismaleimidomethyl ether

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12 December 2020

Site-specific mutagenesis of the alpha subunit of tryptophan synthase from Salmonella typhimurium. Changing arginine 179 to leucine alters the reciprocal transmission of substrate-induced conformational changes between the alpha and beta 2 subunits.

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12 December 2020

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

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12 December 2020

Three-dimensional structure of the tryptophan synthase alpha 2 beta 2 multienzyme complex from Salmonella typhimurium.

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12 December 2020

Identifiers

DOI

10.1007/BF00587570

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27 November 2017

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