(Q43957817)

28 November 2017

title

Toward efficient analysis of mutations in single cells from ethanol-fixed, paraffin-embedded, and immunohistochemically stained tissues (English)

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28 November 2017

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Ernst Heinmöller

1

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28 November 2017

Qiang Liu

2

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28 November 2017

Yuan Sun

3

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28 November 2017

Gudrun Schlake

4

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28 November 2017

Kathleen A Hill

5

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28 November 2017

Larry M Weiss

6

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28 November 2017

Steve S Sommer

7

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28 November 2017

language of work or name

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1 April 2002

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28 November 2017

published in

Laboratory Investigation

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28 November 2017

volume

82

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28 November 2017

issue

4

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28 November 2017

page(s)

443-453

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https://scigraph.springernature.com/pub.10.1038/labinvest.3780437

28 November 2017

exact match

0 references

cites work

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Multiple mutation analyses in single tumor cells with improved whole genome amplification

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Laser capture microdissection

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Allelic drop-out and preferential amplification in single cells and human blastomeres: implications for preimplantation diagnosis of sex and cystic fibrosis.

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Allele dropout in sequential PCR and FISH analysis of single cells (cell recycling).

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Efficiency of single-cell polymerase chain reaction from stained histologic slides and integrity of DNA in archival tissue

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Identification of expressed genes by laser-mediated manipulation of single cells

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Are there low-penetrance TP53 Alleles? evidence from childhood adrenocortical tumors

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Complete loss of wild-type TP53 in a nontransformed human epithelial cell line is preceded by a phase during which a heterozygous TP53 mutant effectively outgrows the homozygous wild-type cells

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A high frequency of sequence alterations is due to formalin fixation of archival specimens

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Allele drop-out can occur in alleles differing by a single nucleotide and is not alleviated by preamplification or minor template increments.

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Cotranslation of activated mutant p53 with wild type drives the wild-type p53 protein into the mutant conformation

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Increasing the denaturation temperature during the first cycles of amplification reduces allele dropout from single cells for preimplantation genetic diagnosis

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A simplified procedure for developing multiplex PCRs

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7 January 2021

Laser capture microdissection of single cells from complex tissues

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7 January 2021

Preferential PCR amplification of alleles: mechanisms and solutions

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7 January 2021

10.1038/LABINVEST.3780437

Identifiers

DOI

1 reference

28 November 2017

1 reference