(Q44183335)

English

Internal sequence analysis of proteins separated on polyacrylamide gels at the submicrogram level: improved methods, applications and gene cloning strategies

scientific article published in July 1990

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scholarly article

1 reference

Europe PubMed Central

30 November 2017

Internal sequence analysis of proteins separated on polyacrylamide gels at the submicrogram level: improved methods, applications and gene cloning strategies (English)

1 reference

Europe PubMed Central

30 November 2017

polyacrylamide gel

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author name string

Tempst P

1

1 reference

Europe PubMed Central

30 November 2017

Link AJ

2

1 reference

Europe PubMed Central

30 November 2017

Riviere LR

3

1 reference

Europe PubMed Central

30 November 2017

Fleming M

4

1 reference

Europe PubMed Central

30 November 2017

Elicone C

5

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Europe PubMed Central

30 November 2017

language of work or name

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publication date

1 July 1990

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Europe PubMed Central

30 November 2017

Electrophoresis

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Europe PubMed Central

30 November 2017

11

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Europe PubMed Central

30 November 2017

7

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Europe PubMed Central

30 November 2017

537-553

1 reference

Europe PubMed Central

30 November 2017

Location of disulphide bridges by diagonal paper electrophoresis. The disulphide bridges of bovine chymotrypsinogen A.

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A microchemical facility for the analysis and synthesis of genes and proteins

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Examination of automated polypeptide sequencing using standard phenyl isothiocyanate reagent and subpicomole high-performance liquid chromatographic analysis

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Protein-blotting on Polybrene-coated glass-fiber sheets. A basis for acid hydrolysis and gas-phase sequencing of picomole quantities of protein previously separated on sodium dodecyl sulfate/polyacrylamide gel.

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A new siliconized-glass fiber as support for protein-chemical analysis of electroblotted proteins

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Microsequence analysis of peptides and proteins. VIII. Improved electroblotting of proteins onto membranes and derivatized glass-fiber sheets

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Extended N-terminal sequencing of proteins of archaebacterial ribosomes blotted from two-dimensional gels onto glass fiber and poly(vinylidene difluoride) membrane.

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Alterations in the phenotype of plant cells studied by NH(2)-terminal amino acid-sequence analysis of proteins electroblotted from two-dimensional gel-separated total extracts.

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Identification of mouse brain proteins after two-dimensional electrophoresis and electroblotting by microsequence analysis and amino acid composition analysis

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Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose

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Sequencing of proteins from two-dimensional gels by using in situ digestion and transfer of peptides to polyvinylidene difluoride membranes: application to proteins associated with sensitization in Aplysia

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Generation of cDNA probes directed by amino acid sequence: cloning of urate oxidase.

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A new solid-state reagent to iodinate proteins. I. Conditions for the efficient labeling of antiserum

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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

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Drying and storage of polyacrylamide slab gels: a simple procedure

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Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications

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A method for the efficient blotting of strongly basic proteins from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose

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Comprehensive, human cellular protein databases and their implication for the study of genome organization and function

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Protein-electroblotting and -microsequencing strategies in generating protein data bases from two-dimensional gels

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Systematic analysis of the total proteins of a mammalian organism: principles, problems and implications for sequencing the human genome

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Generation of peptides suitable for sequence analysis by proteolytic cleavage in reversed-phase high-performance liquid chromatography solvents.

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Determination of the amino acid sequence of porcine trypsin by sequenator analysis

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A procedure for in situ alkylation of cystine residues on glass fiber prior to protein microsequence analysis

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Identification of cysteine-containing peptides in protein digests by high-performance liquid chromatography

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Vapor-phase modification of sulfhydryl groups in proteins

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Micropreparative procedures for high sensitivity sequencing of peptides and proteins

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Use of microbore high-performance liquid chromatography for purifying subnanomole levels of polypeptides for microsequencing

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Molecular cloning and characterization of the ryanodine receptor/junctional channel complex cDNA from skeletal muscle sarcoplasmic reticulum

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A common language for physical mapping of the human genome

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Protein chemists gain a new analytical tool

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Identifiers

10.1002/ELPS.1150110704

1 reference

Europe PubMed Central

30 November 2017

1 reference

Europe PubMed Central

30 November 2017

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