(Q44721778)

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Beta-galactosidases (Escherichia coli) with double substitutions show that Tyr-503 acts independently of Glu-461 but cooperatively with Glu-537.

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scholarly article

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Europe PubMed Central

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5 December 2017

Beta-galactosidases (Escherichia coli) with double substitutions show that Tyr-503 acts independently of Glu-461 but cooperatively with Glu-537. (English)

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Europe PubMed Central

PubMed publication ID

5 December 2017

Escherichia coli

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author name string

Nathan J Roth

1

1 reference

Europe PubMed Central

PubMed publication ID

5 December 2017

Robert M Penner

2

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Europe PubMed Central

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5 December 2017

Reuben E Huber

3

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Europe PubMed Central

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5 December 2017

publication date

1 November 2003

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Europe PubMed Central

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5 December 2017

The Protein Journal

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Europe PubMed Central

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5 December 2017

22

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Europe PubMed Central

PubMed publication ID

5 December 2017

7-8

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Europe PubMed Central

PubMed publication ID

5 December 2017

663-668

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Europe PubMed Central

PubMed publication ID

5 December 2017

https://scigraph.springernature.com/pub.10.1023/b:jopc.0000008731.16884.22

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Tyr-503 of beta-galactosidase (Escherichia coli) plays an important role in degalactosylation

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

High resolution refinement of β-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a structural basis for α-complementation

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

A structural view of the action of Escherichia coli (lacZ) beta-galactosidase

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Glu-537, not Glu-461, is the nucleophile in the active site of (lac Z) beta-galactosidase from Escherichia coli

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Sequence of the Kluyveromyces lactis beta-galactosidase: comparison with prokaryotic enzymes and secondary structure analysis

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Quantitative interpretations of double mutations of enzymes

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Leuconostoc lactis beta-galactosidase is encoded by two overlapping genes

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Cloning and analysis of the β-galactosidase-encoding gene from Clostridium thermosulfurogenes EM1

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Expression and nucleotide sequence of the Clostridium acetobutylicum beta-galactosidase gene cloned in Escherichia coli

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Analysis of the lacZ sequences from two Streptococcus thermophilus strains: comparison with the Escherichia coli and Lactobacillus bulgaricus beta-galactosidase sequences

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Determination of the roles of Glu-461 in beta-galactosidase (Escherichia coli) using site-specific mutagenesis

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Site specific mutants of beta-galactosidase show that Tyr-503 is unimportant in Mg2+ binding but that Glu-461 is very important and may be a ligand to Mg2+.

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Multiple replacements establish the importance of tyrosine-503 in beta-galactosidase (Escherichia coli).

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Expression and nucleotide sequence of the Lactobacillus bulgaricus beta-galactosidase gene cloned in Escherichia coli

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Site-directed mutagenesis of beta-galactosidase (E. coli) reveals that tyr-503 is essential for activity

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Rapid and efficient site-specific mutagenesis without phenotypic selection

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Nucleotide sequence of Klebsiella pneumoniae lac genes

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

m-Fluorotyrosine substitution in beta-galactosidase; evidence for the existence of a catalytically active tyrosine

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Sequence of the ebgA gene of Escherichia coli: comparison with the lacZ gene

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

The mechanism of action of beta-galactosidase. Effect of aglycone nature and -deuterium substitution on the hydrolysis of aryl galactosides

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

The effect of methanol and dioxan on the rates of the β-galactosidase-catalysed hydrolyses of some β-d-galactopyranosides: rate-limiting degalactosylation. The pH-dependence of galactosylation and degalactosylation

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identification of an essential carboxylate group at the active site of lacZ beta-galactosidase from Escherichia coli

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Substitutions for Glu-537 of beta-galactosidase from Escherichia coli cause large decreases in catalytic activity

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Three-dimensional structure of beta-galactosidase from E. coli

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Structure-reactivity relationships for beta-galactosidase (Escherichia coli, lac Z). 3. Evidence that Glu-461 participates in Brønsted acid-base catalysis of beta-D-galactopyranosyl group transfer.

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Methionine 500, the site of covalent attachment of an active site-directed reagent of beta-galactosidase

1 reference

https://pubmed.ncbi.nlm.nih.gov/14714733

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1023/B:JOPC.0000008731.16884.22

1 reference

Europe PubMed Central

PubMed publication ID

5 December 2017

Dimensions Publication ID

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PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

5 December 2017

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