(Q45085376)

English

Construction of gene-targeting vectors: a rapid Mu in vitro DNA transposition-based strategy generating null, potentially hypomorphic, and conditional alleles.

scientific article published in January 2001

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scholarly article

1 reference

Europe PubMed Central

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11252384%20AND%20SRC:MED&resulttype=core&format=json

5 November 2019

Construction of gene-targeting vectors: a rapid Mu in vitro DNA transposition-based strategy generating null, potentially hypomorphic, and conditional alleles (English)

1 reference

Europe PubMed Central

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11252384%20AND%20SRC:MED&resulttype=core&format=json

5 November 2019

Harri Savilahti

5

1 reference

Europe PubMed Central

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5 November 2019

author name string

H Vilen

1

1 reference

Europe PubMed Central

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5 November 2019

S Eerikäinen

2

1 reference

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5 November 2019

J Tornberg

3

1 reference

Europe PubMed Central

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5 November 2019

M S Airaksinen

4

1 reference

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5 November 2019

publication date

1 January 2001

1 reference

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5 November 2019

Transgenic Research

1 reference

Europe PubMed Central

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5 November 2019

10

1 reference

Europe PubMed Central

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5 November 2019

1

1 reference

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5 November 2019

69-80

1 reference

Europe PubMed Central

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5 November 2019

https://scigraph.springernature.com/pub.10.1023/a:1008959231644

0 references

Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouse

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Derivation of completely cell culture-derived mice from early-passage embryonic stem cells

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Segmental genomic replacement in embryonic stem cells by double lox targeting

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A purified mariner transposase is sufficient to mediate transposition in vitro

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Target-selected gene inactivation in Caenorhabditis elegans by using a frozen transposon insertion mutant bank

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Targeted mutagenesis: analysis of phenotype without germ line transmission

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A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis

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cis and trans factors affecting Mos1 mariner evolution and transposition in vitro, and its potential for functional genomics

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Opposite phenotypes of hypomorphic and Y766 phosphorylation site mutations reveal a function for Fgfr1 in anteroposterior patterning of mouse embryos

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Efficient integration of artificial transposons into plasmid targets in vitro: a useful tool for DNA mapping, sequencing and genetic analysis

1 reference

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An Fgf8 mutant allelic series generated by Cre- and Flp-mediated recombination

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A novel method for constructing gene-targeting vectors

1 reference

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12 December 2020

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The phage Mu transpososome core: DNA requirements for assembly and function.

1 reference

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DNA transposition: jumping gene machine, some assembly required.

1 reference

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In vitro transposition of Tn552: a tool for DNA sequencing and mutagenesis

1 reference

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Simplified generation of targeting constructs using ET recombination

1 reference

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General method for plasmid construction using homologous recombination

1 reference

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Transpositional recombination: mechanistic insights from studies of mu and other elements

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The K+/Cl- co-transporter KCC2 renders GABA hyperpolarizing during neuronal maturation

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Mutational analysis of the Pseudomonas syringae pv. tomato hrpA gene encoding Hrp pilus subunit

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Tn10/IS10 transposase purification, activation, and in vitro reaction.

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Molecular characterization of a putative K-Cl cotransporter in rat brain. A neuronal-specific isoform

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An efficient and accurate integration of mini-Mu transposons in vitro: a general methodology for functional genetic analysis and molecular biology applications.

1 reference

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Pentapeptide scanning mutagenesis: random insertion of a variable five amino acid cassette in a target protein

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Non-injection methods for the production of embryonic stem cell-embryo chimaeras

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https://pubmed.ncbi.nlm.nih.gov/11252384

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The neuron-specific K-Cl cotransporter, KCC2. Antibody development and initial characterization of the protein

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An efficient DNA sequencing strategy based on the bacteriophage mu in vitro DNA transposition reaction.

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https://pubmed.ncbi.nlm.nih.gov/11252384

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A simple assay to determine the functionality of Cre or FLP recombination targets in genomic manipulation constructs.

1 reference

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Transposase makes critical contacts with, and is stimulated by, single-stranded DNA at the P element termini in vitro

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Inducible gene targeting in mice using the Cre/lox system

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Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells

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