(Q46616655)

23 December 2017

title

Bacterial expression and one-step purification of an isotope-labeled heterotrimeric G-protein alpha-subunit (English)

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23 December 2017

author name string

Najmoutin G Abdulaev

1

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23 December 2017

Cheng Zhang

2

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23 December 2017

Andy Dinh

3

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23 December 2017

Tony Ngo

4

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23 December 2017

Philip N Bryan

5

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23 December 2017

Danielle M Brabazon

6

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23 December 2017

John P Marino

7

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23 December 2017

Kevin D Ridge

8

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23 December 2017

publication date

1 May 2005

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23 December 2017

Journal of Biomolecular NMR

published in

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23 December 2017

volume

32

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23 December 2017

issue

1

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23 December 2017

page(s)

31-40

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23 December 2017

https://scigraph.springernature.com/pub.10.1007/s10858-005-3340-5

exact match

0 references

cites work

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The intrinsic fluorescence of the alpha subunit of transducin. Measurement of receptor-dependent guanine nucleotide exchange

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Phototransduction: crystal clear

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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

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Structures of active conformations of Gi alpha 1 and the mechanism of GTP hydrolysis

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12 December 2020

The 2.0 A crystal structure of a heterotrimeric G protein

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12 December 2020

Mapping of effector binding sites of transducin alpha-subunit using G alpha t/G alpha i1 chimeras

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12 December 2020

NMRPipe: a multidimensional spectral processing system based on UNIX pipes

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12 December 2020

Site of G protein binding to rhodopsin mapped with synthetic peptides from the alpha subunit

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12 December 2020

The retinal conformation and its environment in rhodopsin in light of a new 2.2 A crystal structure

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12 December 2020

Flow of information in the light-triggered cyclic nucleotide cascade of vision

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12 December 2020

Roles of the transducin alpha-subunit alpha4-helix/alpha4-beta6 loop in the receptor and effector interactions

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12 December 2020

Prodomains and protein folding catalysis

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12 December 2020

Metal chelate affinity chromatography, a new approach to protein fractionation

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12 December 2020

Engineering subtilisin into a fluoride-triggered processing protease useful for one-step protein purification.

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12 December 2020

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12 December 2020

How activated receptors couple to G proteins

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12 December 2020

Tertiary and quaternary structural changes in Gi alpha 1 induced by GTP hydrolysis

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12 December 2020

Characterization of the aluminum and beryllium fluoride species which activate transducin. Analysis of the binding and dissociation kinetics

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12 December 2020

Rhodopsin-stimulated activation-deactivation cycle of transducin: kinetics of the intrinsic fluorescence response of the alpha subunit

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12 December 2020

The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma 2

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12 December 2020

Solution structure of the GTPase activating domain of alpha s.

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12 December 2020

A simplification of the protein assay method of Lowry et al. which is more generally applicable

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12 December 2020

Complete 1H, 13C, and 15N assignments and secondary structure of the GTPase activating domain of Gs.

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12 December 2020

19F and 31P NMR spectroscopy of G protein alpha subunits. Mechanism of activation by Al3+ and F-.

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12 December 2020

Mapping of contact sites in complex formation between transducin and light-activated rhodopsin by covalent crosslinking: use of a photoactivatable reagent

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12 December 2020

Functionally discrete mimics of light-activated rhodopsin identified through expression of soluble cytoplasmic domains

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12 December 2020

Attenuated T2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution

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12 December 2020

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12 December 2020

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12 December 2020

Affinity purification of histidine-tagged proteins

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12 December 2020

Tryptophan W207 in transducin T alpha is the fluorescence sensor of the G protein activation switch and is involved in the effector binding

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12 December 2020

Structural determinants for activation of the alpha-subunit of a heterotrimeric G protein

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12 December 2020

Recombinant protein expression in Escherichia coli

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12 December 2020

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12 December 2020

Structure of bovine rhodopsin in a trigonal crystal form.

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12 December 2020

Functional role of internal water molecules in rhodopsin revealed by X-ray crystallography.

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12 December 2020

Effects of differential sulfhydryl group-specific labeling on the rhodopsin and guanine nucleotide binding activities of transducin

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12 December 2020

Structural determinants for regulation of phosphodiesterase by a G protein at 2.0 A

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12 December 2020

GTPase mechanism of Gproteins from the 1.7-A crystal structure of transducin alpha-GDP-AIF-4

1 reference

12 December 2020

10.1007/S10858-005-3340-5

Identifiers

DOI

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23 December 2017

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23 December 2017