(Q48274494)

10 February 2018

title

High-efficiency DNA ligation for clamp attachment without polymerase chain reaction (English)

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10 February 2018

author name string

Andrea S Kim

series ordinal

1

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10 February 2018

Gerald P Holmquist

series ordinal

2

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10 February 2018

William G Thilly

series ordinal

3

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10 February 2018

language of work or name

English

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publication date

1 November 2002

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10 February 2018

published in

Analytical Biochemistry

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10 February 2018

volume

310

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10 February 2018

issue

2

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10 February 2018

page(s)

179-185

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10 February 2018

DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory

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Modification of the melting properties of duplex DNA by attachment of a GC-rich DNA sequence as determined by denaturing gradient gel electrophoresis

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Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis

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Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes

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Comprehensive detection of single base changes in human genomic DNA using denaturing gradient gel electrophoresis and a GC clamp

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Psoralen-modified oligonucleotide primers improve detection of mutations by denaturing gradient gel electrophoresis and provide an alternative to GC-clamping

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7 January 2021

Identifiers

DOI

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10 February 2018

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10 February 2018