(Q48942300)

English

Combination of two-dimensional gel electrophoresis with microsequencing and amino acid composition analysis: improvement of speed and sensitivity in protein characterization.

scientific article published in July 1990

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scholarly article

1 reference

Europe PubMed Central

17 February 2018

http://europepmc.org/abstract/MED/2226410

Combination of two-dimensional gel electrophoresis with microsequencing and amino acid composition analysis: improvement of speed and sensitivity in protein characterization. (English)

1 reference

Europe PubMed Central

17 February 2018

http://europepmc.org/abstract/MED/2226410

author name string

Eckerskorn C

1

1 reference

Europe PubMed Central

17 February 2018

http://europepmc.org/abstract/MED/2226410

Lottspeich F

2

1 reference

Europe PubMed Central

17 February 2018

http://europepmc.org/abstract/MED/2226410

publication date

1 July 1990

1 reference

Europe PubMed Central

17 February 2018

http://europepmc.org/abstract/MED/2226410

Electrophoresis

1 reference

Europe PubMed Central

17 February 2018

http://europepmc.org/abstract/MED/2226410

11

1 reference

Europe PubMed Central

17 February 2018

http://europepmc.org/abstract/MED/2226410

554-561

1 reference

Europe PubMed Central

17 February 2018

http://europepmc.org/abstract/MED/2226410

7

1 reference

Europe PubMed Central

17 February 2018

http://europepmc.org/abstract/MED/2226410

High-sensitivity sequencing with a gas-phase sequenator

1 reference

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Systematic analysis of the total proteins of a mammalian organism: principles, problems and implications for sequencing the human genome

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Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other en

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Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis

1 reference

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A high-performance liquid chromatography procedure for recovering subnanomole amounts of protein from SDS-gel electroeluates for gas-phase sequence analysis

1 reference

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A high-yield method for the isolation of hydrophobic proteins and peptides from polyacrylamide gels for protein sequencing

1 reference

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Micropreparative procedures for high sensitivity sequencing of peptides and proteins

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Polypeptide microsequence analysis with the commercially available gas-phase sequencer

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A microbore high-performance liquid chromatography strategy for the purification of polypeptides for gas-phase sequence analysis. Structural studies on the murine transferrin receptor

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Protein-blotting on Polybrene-coated glass-fiber sheets. A basis for acid hydrolysis and gas-phase sequencing of picomole quantities of protein previously separated on sodium dodecyl sulfate/polyacrylamide gel.

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A new siliconized-glass fiber as support for protein-chemical analysis of electroblotted proteins

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Determination of amino acid compositions and NH2-terminal sequences of peptides electroblotted onto PVDF membranes from tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis: application to peptide mapping of human complement component C

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Amino acid analysis on polyvinylidene difluoride membranes.

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Direct amino acid analysis of proteins electroblotted onto polyvinylidene difluoride membrane from sodium dodecyl sulfate-polyacrylamide gel.

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Identification of mouse brain proteins after two-dimensional electrophoresis and electroblotting by microsequence analysis and amino acid composition analysis

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Internal amino acid sequencing of proteins by in situ cyanogen bromide cleavage in polyacrylamide gels

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Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose

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A simple in situ cyanogen bromide cleavage method to obtain internal amino acid sequence of proteins electroblotted to polyvinyldifluoride membranes

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Blotting of proteins onto Immobilon membranes. In situ characterization and comparison with high-performance liquid chromatography

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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

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Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

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7 January 2021

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Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate.

1 reference

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The current state of two-dimensional electrophoresis with immobilized pH gradients

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Peptide mapping by polyacrylamide gel electrophoresis after cleavage at aspartyl-prolyl peptide bonds in sodium dodecyl sulfate-containing buffers

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Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose

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Blotting efficiency investigated by using two-dimensional electrophoresis, hydrophobic membranes and proteins from different sources.

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Measurement of free amino acids in human biological fluids by high-performance liquid chromatography

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A Rapid Vapor-Phase Acid (Hydrochloric Acid and Trifluoroacetic Acid) Hydrolysis of Peptide and Protein

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Report of workshop on cellular protein databases derived from two-dimensional polyacrylamide gel electrophoresis

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Identifiers

10.1002/ELPS.1150110705

1 reference

Europe PubMed Central

17 February 2018

http://europepmc.org/abstract/MED/2226410

1 reference

Europe PubMed Central

17 February 2018

http://europepmc.org/abstract/MED/2226410

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