(Q49049417)

18 February 2018

title

Assembly-disassembly purification and characterization of microtubule protein without glycerol (English)

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18 February 2018

author name string

Murphy DB

series ordinal

1

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18 February 2018

publication date

1 January 1982

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18 February 2018

published in

Methods in Cell Biology

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18 February 2018

volume

24

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18 February 2018

page(s)

31-49

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18 February 2018

Arrangement of high molecular weight associated proteins on purified mammalian brain microtubules

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Isolation of bovine brain microtubule protein without glycerol: Polymerization kinetics change during purification cycles

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Purification of tubulin and associated high molecular weight proteins from porcine brain and characterization of microtubule assembly in vitro

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Purification of tau, a microtubule-associated protein that induces assembly of microtubules from purified tubulin

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Immunoflourescent staining of cytoplasmic and spindle microtubules in mouse fibroblasts with antibody to tau protein

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Ultrastructural localization of the high molecular weight proteins associated with in vitro-assembled brain microtubules

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Binding of glycerol by microtubule protein

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In vitro assembly of tubulin from nonneural cells (Ehrlich ascites tumor cells)

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Polycation-induced assembly of purified tubulin

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A dynein-like protein associated with neurotubules.

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A new ATPase in cytoplasmic microtubule preparations

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Evidence for actin filament-microtubule interaction mediated by microtubule-associated proteins

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In vitro assembly of pure tubulin into microtubules in the absence of microtubule-associated proteins and glycerol

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Tubulin-Associated Nucleoside Diphosphokinase

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The periodic association of MAP2 with brain microtubules in vitro

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The reconstitution of microtubules from purified calf brain tubulin

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Sulfhydryls and the in vitro polymerization of tubulin

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Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro

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Purification of microtubule protein from beef brain and comparison of the assembly requirements for neuronal microtubules isolated from beef and hog.

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Role of tubulin-associated proteins in microtubule nucleation and elongation

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Identity and polymerization-stimulatory activity of the nontubulin proteins associated with microtubules

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Characterization of microtubule assembly in porcine brain extracts by viscometry

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Ionic and nucleotide requirements for microtubule polymerization in vitro

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Immunofluorescence and electron microscopy of the cytoplasmic surface of the human erythrocyte membrane and its interaction with Sendai virus

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7 January 2021

Comparison of the sedimentation properties of microtubule protein oligomers prepared by two different procedures

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7 January 2021

Microtubule Assembly in the Absence of Added Nucleotides

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Immunofluorescence Localization of Proteins of High Molecular Weight Along Intracellular Microtubules

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Localisation of the major high-molecular-weight protein on microtubules in vitro and in cultured cells

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7 January 2021

Decoration and stabilization of intact, smooth-walled microtubules with microtubule-associated proteins

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7 January 2021

Cyclic AMP-dependent endogenous phosphorylation of a microtubule-associated protein

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In vitro polymerization of microtubules from HeLa cells

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7 January 2021

A protein factor essential for microtubule assembly

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7 January 2021

Microtubule Formation in vitro in Solutions Containing Low Calcium Concentrations

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7 January 2021

Reversible in vitro polymerization of tubulin from a cultured cell line (rat glial cell clone C6)

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7 January 2021

Separation of tubulin from microtubule-associated proteins on phosphocellulose. Accompanying alterations in concentrations of buffer components

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7 January 2021

Tubulin does not bind glycerol irreversibly

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7 January 2021

10.1016/S0091-679X(08)60646-9

Identifiers

DOI

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1 reference

18 February 2018