(Q50108023)

25 February 2018

title

Cloning and hemolysin-mediated secretory expression of a codon-optimized synthetic human interleukin-6 gene in Escherichia coli. (English)

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25 February 2018

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Yuanyi Li

1

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25 February 2018

Carol X Chen

series ordinal

2

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25 February 2018

Bernd-Ulrich von Specht

series ordinal

3

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25 February 2018

Heinz P Hahn

series ordinal

4

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25 February 2018

publication date

1 August 2002

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25 February 2018

Protein Expression and Purification

published in

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25 February 2018

volume

25

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25 February 2018

issue

3

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25 February 2018

page(s)

437-447

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25 February 2018

Interleukin-6 in biology and medicine

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Protein secretion by Gram-negative bacterial ABC exporters--a review

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High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product

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Oral immunization using live attenuated Salmonella spp. as carriers of foreign antigens

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Suppression of the negative effect of minor arginine codons on gene expression; preferential usage of minor codons within the first 25 codons of the Escherichia coli genes

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Expression of the murine interleukin-4 gene in an attenuated aroA strain of Salmonella typhimurium: persistence and immune response in BALB/c mice and susceptibility to macrophage killing

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Formation of disulphide bonds during secretion of proteins through the periplasmic-independent type I pathway

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A synthetic gene coding for the green fluorescent protein (GFP) is a versatile reporter in Chlamydomonas reinhardtii

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Development of antigen-delivery systems, based on the Escherichia coli hemolysin secretion pathway.

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Consecutive low-usage leucine codons block translation only when near the 5' end of a message in Escherichia coli.

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Codon optimization of the gene encoding a domain from human type 1 neurofibromin protein results in a threefold improvement in expression level in Escherichia coli

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Biological and clinical aspects of interleukin 6.

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Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin

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Molecular analysis of hemolysin-mediated secretion of a human interleukin-6 fusion protein in Salmonella typhimurium.

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A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels

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7 January 2021

Roles of disulfide bonds in recombinant human interleukin 6 conformation

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7 January 2021

Post-translational modifications of human interleukin-6

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7 January 2021

Saturation mutagenesis of the human interleukin 6 receptor-binding site: implications for its three-dimensional structure

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1.9 A crystal structure of interleukin 6: implications for a novel mode of receptor dimerization and signaling

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Engineering human interleukin-6 to obtain variants with strongly enhanced bioactivity.

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Optimized heterologous expression of the polymorphic human glutathione transferase M1-1 based on silent mutations in the corresponding cDNA.

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Design, synthesis and expression of a human interleukin-2 gene incorporating the codon usage bias found in highly expressed Escherichia coli genes

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7 January 2021

Solution structure of recombinant human interleukin-6

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7 January 2021

Purification and characterization of a recombinant murine interleukin-6. Isolation of N- and C-terminally truncated forms

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Identifiers

DOI

1 reference

25 February 2018

1 reference

25 February 2018