(Q50646033)

17 March 2018

title

A protocol for constructing gene targeting vectors: generating knockout mice for the cadherin family and beyond. (English)

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17 March 2018

0 references

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Sen Wu

1

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17 March 2018

Guoxin Ying

series ordinal

2

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17 March 2018

Qiang Wu

series ordinal

3

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17 March 2018

Mario R Capecchi

series ordinal

4

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17 March 2018

publication date

1 January 2008

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17 March 2018

published in

Nature Protocols

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17 March 2018

volume

3

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17 March 2018

issue

6

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17 March 2018

page(s)

1056-1076

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17 March 2018

https://scigraph.springernature.com/pub.10.1038/nprot.2008.70

exact match

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31 March 2020

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31 March 2020

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31 March 2020

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31 March 2020

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31 March 2020

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31 March 2020

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31 March 2020

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31 March 2020

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31 March 2020

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31 March 2020

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31 March 2020

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31 March 2020

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31 March 2020

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31 March 2020

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Fgf8 is required for outgrowth and patterning of the limbs

31 March 2020

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Restoration of p53 function leads to tumour regression in vivo

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Motoneurons and oligodendrocytes are sequentially generated from neural stem cells but do not appear to share common lineage-restricted progenitors in vivo

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Evidence for an expansion-based temporal Shh gradient in specifying vertebrate digit identities

31 March 2020

1 reference

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Efficient recombination in diverse tissues by a tamoxifen-inducible form of Cre: a tool for temporally regulated gene activation/inactivation in the mouse

31 March 2020

1 reference

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Temporally-controlled site-specific mutagenesis in the basal layer of the epidermis: comparison of the recombinase activity of the tamoxifen-inducible Cre-ER(T) and Cre-ER(T2) recombinases

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Maintenance of functional equivalence during paralogous Hox gene evolution

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Transcriptional activation by tetracyclines in mammalian cells

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Stringent control of gene expression in vivo by using novel doxycycline-dependent trans-activators

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Generalized lacZ expression with the ROSA26 Cre reporter strain

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

A conditional mouse model of synovial sarcoma: insights into a myogenic origin

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Reexamination of Gene Targeting Frequency as a Function of the Extent of Homology between the Targeting Vector and the Target Locus

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Target frequency and integration pattern for insertion and replacement vectors in embryonic stem cells

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

A Cre/loxP-deleter transgenic line in mouse strain 129S1/SvImJ.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Targeting genes for self-excision in the germ line

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

High frequency targeting of genes to specific sites in the mammalian genome

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Production of chimaeric mice containing embryonic stem (ES) cells carrying a homoeobox Hox 1.1 allele mutated by homologous recombination.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Homologous recombination in human embryonic stem cells.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Manipulating the mammalian genome by homologous recombination

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Patterns of integration of DNA microinjected into cultured mammalian cells: evidence for homologous recombination between injected plasmid DNA molecules

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Targetted correction of a mutant HPRT gene in mouse embryonic stem cells

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Developmental and adult phenotyping directly from mutant embryonic stem cells

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Derivation of completely cell culture-derived mice from early-passage embryonic stem cells

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Gene-targeting studies of mammalian behavior: is it the mutation or the background genotype?

31 March 2020

1 reference

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Knockout mice: simple solutions to the problems of genetic background and flanking genes

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

To knockout in 129 or in C57BL/6: that is the question

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Improved generation of germline-competent embryonic stem cell lines from inbred mouse strains

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Use of coisogenic host blastocysts for efficient establishment of germline chimeras with C57BL/6J ES cell lines

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Engineering mouse chromosomes with Cre-loxP: range, efficiency, and somatic applications

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Modulation of gene activity by consecutive gene targeting of one creatine kinase M allele in mouse embryonic stem cells

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Enrichment and efficient screening of ES cells containing a targeted mutation: the use of DT-A gene with the polyadenylation signal as a negative selection maker

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Homologous recombination at c-fyn locus of mouse embryonic stem cells with use of diphtheria toxin A-fragment gene in negative selection

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Non-injection methods for the production of embryonic stem cell-embryo chimaeras

31 March 2020

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https://api.crossref.org/works/10.1038%2FNPROT.2008.70

High efficiency transformation of Escherichia coli with plasmids

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

One-hour downward alkaline capillary transfer for blotting of DNA and RNA

31 March 2020

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https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and tris (HotSHOT).

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Comparative DNA sequence analysis of mouse and human protocadherin gene clusters

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Comparative genomics and diversifying selection of the clustered vertebrate protocadherin genes

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Genomic organization of the family of CNR cadherin genes in mice and humans

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

MEGF1/fat2 proteins containing extraordinarily large extracellular domains are localized to thin parallel fibers of cerebellar granule cells

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Temporal and spatial expression profiles of the Fat3 protein, a giant cadherin molecule, during mouse development.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

Expression of mouse dchs1, fjx1, and fat-j suggests conservation of the planar cell polarity pathway identified in Drosophila.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.70

31 March 2020

Identifiers

DOI

10.1038/NPROT.2008.70

1 reference

17 March 2018

1 reference

17 March 2018