(Q50773333)

19 March 2018

title

Secretion and surface display of green fluorescent protein using the yeast Saccharomyces cerevisiae. (English)

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19 March 2018

main subject

Saccharomyces cerevisiae

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Dagang Huang

1

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19 March 2018

Eric V Shusta

series ordinal

2

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19 March 2018

publication date

1 March 2005

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19 March 2018

published in

Biotechnology Progress

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19 March 2018

volume

21

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19 March 2018

issue

2

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19 March 2018

page(s)

349-357

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19 March 2018

Insulin targeting to the regulated secretory pathway after fusion with green fluorescent protein and firefly luciferase

cites work

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The AGA1 product is involved in cell surface attachment of the Saccharomyces cerevisiae cell adhesion glycoprotein a-agglutinin

21 January 2018

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Understanding, improving and using green fluorescent proteins

21 January 2018

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7 January 2021

Green fluorescent protein (GFP): applications, structure, and related photophysical behavior

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7 January 2021

The green fluorescent protein

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7 January 2021

Biotechnological applications of green fluorescent protein

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7 January 2021

Impediments to secretion of green fluorescent protein and its fusion from Saccharomyces cerevisiae

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7 January 2021

The green fluorescent protein targets secretory proteins to the yeast vacuole

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7 January 2021

Viral preprotoxin signal sequence allows efficient secretion of green fluorescent protein by Candida glabrata, Pichia pastoris, Saccharomyces cerevisiae, and Schizosaccharomyces pombe

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7 January 2021

Monitoring phase-specific gene expression in Histoplasma capsulatum with telomeric GFP fusion plasmids

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7 January 2021

Functional phytohemagglutinin (PHA) and Galanthus nivalis agglutinin (GNA) expressed in Pichia pastoris correct N-terminal processing and secretion of heterologous proteins expressed using the PHA-E signal peptide

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7 January 2021

Secretion of heterologous proteins from Schizosaccharomyces pombe using the homologous leader sequence of pho1+ acid phosphatase

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7 January 2021

Visualizing and quantifying protein secretion using a Renilla luciferase-GFP fusion protein

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7 January 2021

Secretion of green fluorescent protein from recombinant baculovirus-infected insect cells

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7 January 2021

Construction of an engineered yeast with glucose-inducible emission of green fluorescence from the cell surface

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7 January 2021

Co-expression of molecular chaperones does not improve the heterologous expression of mammalian G-protein coupled receptor expression in yeast

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7 January 2021

Green fluorescent protein-cell wall fusion proteins are covalently incorporated into the cell wall of Saccharomyces cerevisiae

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7 January 2021

Creation of cell surface-engineered yeast that display different fluorescent proteins in response to the glucose concentration

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7 January 2021

Affinity selection of target cells from cell surface displayed libraries: a novel procedure using thermo-responsive magnetic nanoparticles

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7 January 2021

Yeast surface display for directed evolution of protein expression, affinity, and stability

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7 January 2021

Yeast surface display for screening combinatorial polypeptide libraries

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7 January 2021

FACS-optimized mutants of the green fluorescent protein (GFP)

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7 January 2021

Secretion of human epidermal growth factor from Saccharomyces cerevisiae using synthetic leader sequences.

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7 January 2021

Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity

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7 January 2021

Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure

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7 January 2021

Measurement of protein by spectrophotometry at 205 nm.

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7 January 2021

Increasing the secretory capacity of Saccharomyces cerevisiae for production of single-chain antibody fragments

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7 January 2021

Directed evolution of a stable scaffold for T-cell receptor engineering.

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7 January 2021

Deglycosylation of asparagine-linked glycans by peptide:N-glycosidase F.

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7 January 2021

Delineation of functional regions within the subunits of the Saccharomyces cerevisiae cell adhesion molecule a-agglutinin.

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7 January 2021

Prepro-leaders lacking N-linked glycosylation for secretory expression in the yeast Saccharomyces cerevisiae

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7 January 2021

Expression level tuning for optimal heterologous protein secretion in Saccharomyces cerevisiae.

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7 January 2021

Global unfolding of a substrate protein by the Hsp100 chaperone ClpA.

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7 January 2021

Chaperonin-mediated folding of green fluorescent protein.

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7 January 2021

Protein disulfide isomerase, but not binding protein, overexpression enhances secretion of a non-disulfide-bonded protein in yeast

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7 January 2021

A new method for predicting signal sequence cleavage sites

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7 January 2021

Quantitative evaluation of the enhanced green fluorescent protein displayed on the cell surface of Saccharomyces cerevisiae by fluorometric and confocal laser scanning microscopic analyses

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7 January 2021

Integrated bioprocessing in Saccharomyces cerevisiae using green fluorescent protein as a fusion partner

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7 January 2021

Improved green fluorescent protein by molecular evolution using DNA shuffling

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7 January 2021

Mutations that suppress the thermosensitivity of green fluorescent protein

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7 January 2021

S147P green fluorescent protein: a less thermosensitive green fluorescent protein variant.

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7 January 2021

Localization, trafficking, and temperature-dependence of the Aequorea green fluorescent protein in cultured vertebrate cells.

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7 January 2021

A novel mutation which enhances the fluorescence of green fluorescent protein at high temperatures

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7 January 2021

Rapid protein-folding assay using green fluorescent protein

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7 January 2021

Identifiers

DOI

10.1021/BP0497482

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19 March 2018

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19 March 2018