(Q51767602)

11 April 2018

title

Utilization of non-AUG initiation codons in a flow cytometric method for efficient selection of recombinant cell lines. (English)

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11 April 2018

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Victor R Cairns

1

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Christine T DeMaria

2

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Francis Poulin

3

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José Sancho

4

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Ping Liu

5

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Jin Zhang

6

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Juanita Campos-Rivera

7

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Kenneth P Karey

8

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Scott Estes

9

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publication date

16 June 2011

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11 April 2018

Biotechnology and Bioengineering

published in

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11 April 2018

volume

108

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issue

11

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11 April 2018

page(s)

2611-2622

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High-throughput clonal selection of recombinant CHO cells using a dominant selectable and amplifiable metallothionein-GFP fusion protein.

cites work

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Establishment of a strategy for the rapid generation of a monoclonal antibody against the human protein SNEV (hNMP200) by flow-cytometric cell sorting

21 January 2018

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A simple method for enriching populations of transfected CHO cells for cells of higher specific productivity

21 January 2018

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Selection methods for high-producing mammalian cell lines.

21 January 2018

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Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins

21 January 2018

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Accelerated clone selection for recombinant CHO CELLS using a FACS-based high-throughput screen

21 January 2018

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Context effects and inefficient initiation at non-AUG codons in eucaryotic cell-free translation systems

21 January 2018

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Downstream secondary structure facilitates recognition of initiator codons by eukaryotic ribosomes

21 January 2018

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A second look at cellular mRNA sequences said to function as internal ribosome entry sites

21 January 2018

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Generation of mammalian cells stably expressing multiple genes at predetermined levels.

21 January 2018

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Initiation of translation at CUG, GUG, and ACG codons in mammalian cells

21 January 2018

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High level expression of proteins using sequences from the ferritin heavy chain gene locus

21 January 2018

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High-level expression of proteins in mammalian cells using transcription regulatory sequences from the Chinese hamster EF-1alpha gene

21 January 2018

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Selection of high-producing CHO cells using NPT selection marker with reduced enzyme activity.

21 January 2018

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Comparison of humanized IgG and FvFc anti-CD3 monoclonal antibodies expressed in CHO cells.

21 January 2018

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Accelerated cell line development using two-color fluorescence activated cell sorting to select highly expressing antibody-producing clones

21 January 2018

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A novel, high stringency selection system allows screening of few clones for high protein expression

21 January 2018

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High levels of protein expression using different mammalian CMV promoters in several cell lines

21 January 2018

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Translational efficiency of EMCV IRES in bicistronic vectors is dependent upon IRES sequence and gene location

21 January 2018

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21 January 2018

Identifiers

DOI

10.1002/BIT.23219

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11 April 2018

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11 April 2018