(Q54041684)

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Cysteine-scanning mutagenesis of transmembrane segments 4 and 5 of the Tn10-encoded metal-tetracycline/H+ antiporter reveals a permeability barrier in the middle of a transmembrane water-filled channel.

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9 November 2019

Cysteine-scanning mutagenesis of transmembrane segments 4 and 5 of the Tn10-encoded metal-tetracycline/H+ antiporter reveals a permeability barrier in the middle of a transmembrane water-filled channel (English)

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Europe PubMed Central

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9 November 2019

transmembrane protein

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S Iwaki

1

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9 November 2019

N Tamura

2

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9 November 2019

T Kimura-Someya

3

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9 November 2019

S Nada

4

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9 November 2019

A Yamaguchi

5

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9 November 2019

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1 July 2000

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9 November 2019

Journal of Biological Chemistry

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9 November 2019

275

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9 November 2019

22704-22712

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9 November 2019

30

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9 November 2019

Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Active efflux mechanisms for antimicrobial resistance

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Proton-linked sugar transport systems in bacteria

1 reference

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21 January 2018

Membrane topology of the transposon 10-encoded metal-tetracycline/H+ antiporter as studied by site-directed chemical labeling

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Cysteine-scanning mutagenesis around transmembrane segment III of Tn10-encoded metal-tetracycline/H+ antiporter

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Cysteine-scanning mutagenesis around transmembrane segment VI of Tn10-encoded metal-tetracycline/H(+) antiporter.

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Determination of a transmembrane segment using cysteine-scanning mutants of transposon Tn10-encoded metal-tetracycline/H+ antiporter.

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Topology of the region surrounding Glu681 of human AE1 protein, the erythrocyte anion exchanger

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Scanning Cysteine Accessibility of EmrE, an H+-coupled Multidrug Transporter fromEscherichia coli, Reveals a Hydrophobic Pathway for Solutes

1 reference

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21 January 2018

Cysteine scanning mutagenesis of putative transmembrane helices IX and X in the lactose permease of Escherichia coli

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Cysteine scanning mutagenesis of putative helix XI in the lactose permease of Escherichia coli

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Cysteine-scanning mutagenesis of putative helix VII in the lactose permease of Escherichia coli

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Cysteine scanning mutagenesis of helix V in the lactose permease of Escherichia coli

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Cysteine-scanning mutagenesis of transmembrane domain XII and the flanking periplasmic loop in the lactose permease of EScherichia coli.

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Cysteine-scanning mutagenesis of helix II and flanking hydrophilic domains in the lactose permease of Escherichia coli

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

The role of helix VIII in the lactose permease of Escherichia coli: I. Cys-scanning mutagenesis

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Residues in the pathway through a membrane transporter

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Site-directed chemical modification of cysteine-scanning mutants as to transmembrane segment II and its flanking regions of the Tn10-encoded metal-tetracycline/H+ antiporter reveals a transmembrane water-filled channel

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Mutational analysis and molecular modelling of an amino acid sequence motif conserved in antiporters but not symporters in a transporter superfamily

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

A new tetracycline resistance determinant, Tet H, from Pasteurella multocida specifying active efflux of tetracycline

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

The pur8 gene from the pur cluster of Streptomyces alboniger encodes a highly hydrophobic polypeptide which confers resistance to puromycin

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Rapid and efficient site-specific mutagenesis without phenotypic selection

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Site-specificity of the second-site suppressor mutation of the Asp-285-->Asn mutant of metal-tetracycline/H+ antiporter of Escherichia coli and the effects of amino acid substitutions at the first and second sites.

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

An oligodeoxyribonucleotide-directed dual amber method for site-directed mutagenesis.

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Plasmids with a kanamycin-resistance gene for site-directed mutagenesis using the oligodeoxyribonucleotide-directed dual amber method.

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Orientation of the carboxyl terminus of the transposon Tn10-encoded tetracycline resistance protein in Escherichia coli

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Translation of MuLV and MSV RNAs in nuclease-treated reticulocyte extracts: Enhancement of the gag-pol polypeptide with yeast suppressor tRNA

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Roles of conserved arginine residues in the metal-tetracycline/H+ antiporter of Escherichia coli.

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Role of the charge interaction between Arg(70) and Asp(120) in the Tn10-encoded metal-tetracycline/H(+) antiporter of Escherichia coli.

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Membrane topology of a cysteine-less mutant of human P-glycoprotein

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Substrate-induced acceleration of N-ethylmaleimide reaction with the Cys-65 mutant of the transposon Tn10-encoded metal-tetracycline/H+ antiporter depends on the interaction of Asp-66 with the substrate.

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Helix packing in polytopic membrane proteins: role of glycine in transmembrane helix association

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Design of a membrane transport protein for fluorescence spectroscopy

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Role of glycine residues in the structure and function of lactose permease, an Escherichia coli membrane transport protein

1 reference

https://api.crossref.org/works/10.1074%2FJBC.M910354199

21 January 2018

Identifiers

10.1074/JBC.M910354199

1 reference

Europe PubMed Central

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:10930423%20AND%20SRC:MED&resulttype=core&format=json

9 November 2019

1 reference

Europe PubMed Central

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:10930423%20AND%20SRC:MED&resulttype=core&format=json

9 November 2019

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