(Q54576819)

1 June 2018

title

Coexpression of a human P450 (CYP3A4) and P450 reductase generates a highly functional monooxygenase system in Escherichia coli. (English)

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1 June 2018

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Blake JA

1

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1 June 2018

Pritchard M

2

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1 June 2018

Ding S

3

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1 June 2018

Smith GC

4

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1 June 2018

Burchell B

5

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1 June 2018

Wolf CR

6

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1 June 2018

Friedberg T

7

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1 June 2018

language of work or name

English

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publication date

1 November 1996

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1 June 2018

published in

FEBS Letters

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1 June 2018

volume

397

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1 June 2018

issue

2-3

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1 June 2018

page(s)

210-214

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1 June 2018

Oxidation of toxic and carcinogenic chemicals by human cytochrome P-450 enzymes

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Dissection of NADPH-cytochrome P450 oxidoreductase into distinct functional domains

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Stable expression of rat cytochrome P-450IIB1 cDNA in Chinese hamster cells (V79) and metabolic activation of aflatoxin B1.

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The development of a human cell line stably expressing human CYP3A4: role in the metabolic activation of aflatoxin B1 and comparison to CYP1A2 and CYP2A3.

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Expression of modified human cytochrome P450 3A4 in Escherichia coli and purification and reconstitution of the enzyme.

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Expression and enzymatic activity of recombinant cytochrome P450 17 alpha-hydroxylase in Escherichia coli

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Purification and characterization of recombinant-expressed cytochrome P450 2C3 from Escherichia coli: 2C3 encodes the 6 beta-hydroxylase deficient form of P450 3b.

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Activation of procarcinogens by human cytochrome P450 enzymes expressed in Escherichia coli. Simplified bacterial systems for genotoxicity assays.

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Roles of divalent metal ions in oxidations catalyzed by recombinant cytochrome P450 3A4 and replacement of NADPH--cytochrome P450 reductase with other flavoproteins, ferredoxin, and oxygen surrogates.

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7 Purification and properties of NADPH-Cytochrome P-450 reductase

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The colorimetric estimation of formaldehyde by means of the Hantzsch reaction

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Expression of house fly CYP6A1 and NADPH-cytochrome P450 reductase in Escherichia coli and reconstitution of an insecticide-metabolizing P450 system.

21 January 2018

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Relative expression of cytochrome P450 isoenzymes in human liver and association with the metabolism of drugs and xenobiotics

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Expression of Cytochrome-P450-3A5 in Escherichia Coli: Effects of 5′ Modification, Purification, Spectral Characterization, Reconstitution Conditions, and Catalytic Activities

21 January 2018

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Roles of Cytochrome b5in the Oxidation of Testosterone and Nifedipine by Recombinant Cytochrome P450 3A4 and by Human Liver Microsomes

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Coexpression of mammalian cytochrome P450 and reductase in Escherichia coli

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Assay of formaldehyde generated during microsomal oxidation reactions

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Five of 12 forms of vaccinia virus-expressed human hepatic cytochrome P450 metabolically activate aflatoxin B1

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10.1016/S0014-5793(96)01196-9

7 January 2021

Identifiers

DOI

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1 June 2018

PubMed ID

8955349

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1 June 2018