(Q57961057)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11582813%20AND%20SRC:MED&resulttype=core&format=json

11 November 2019

title

Fast, facile, hypersensitive assays for ribonucleolytic activity (English)

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11 November 2019

1

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11 November 2019

Mark A. Behlke

5

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11 November 2019

Ronald T. Raines

7

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11 November 2019

author name string

B R Kelemen

2

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11 November 2019

T A Klink

3

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11 November 2019

R Y Sweeney

4

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11 November 2019

S R Eubanks

6

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11 November 2019

language of work or name

English

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publication date

1 January 2001

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11 November 2019

published in

Methods in Enzymology

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11 November 2019

volume

341

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11 November 2019

page(s)

81-94

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11 November 2019

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

Spectrophotometric assay of bovine pancreatic ribonuclease by the use of cytidine 2':3'-phosphate

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

The role of 2',3'-cyclic phosphodiesters in the bovine pancreatic ribonuclease A catalysed cleavage of RNA: intermediates or products?

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

Energetics of Catalysis by Ribonucleases: Fate of the 2',3'-Cyclic Phosphodiester Intermediate

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

A general ribonuclease assay using methylene blue.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

Activity staining of nucleolytic enzymes after sodium dodecyl sulfate-polyacrylamide gel electrophoresis: Use of aqueous isopropanol to remove detergent from gels

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

Ribonuclease S-peptide as a carrier in fusion proteins.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

A versatile negative-staining ribonuclease zymogram

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

The zymogram method for detection of ribonucleases after isoelectric focusing: Analysis of multiple forms of human, bovine, and microbial enzymes

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

Activity measurement and multiplicity detection of human secretory-type ribonuclease based on polycytidylic acid/ethidium bromide fluorescence

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

Extending the Limits to Enzymatic Catalysis: Diffusion of Ribonuclease A in One Dimension†

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

Application of the indigogenic principle for the histochemical demonstration of ribonuclease

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

U-3'-BCIP: a chromogenic substrate for the detection of RNase A in recombinant DNA expression systems

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

Experimental charge measurement at leaving oxygen in the bovine ribonuclease A catalyzed cyclization of uridine 3'-phosphate aryl esters

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

Value of general Acid-base catalysis to ribonuclease a.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

Engineering ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln11.

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

A novel fluorogenic substrate for ribonucleases. Synthesis and enzymatic characterization

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

A fluorescence-based assay for ribonuclease A activity

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

Hypersensitive substrate for ribonucleases

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

Isolation of cytoplasmic RNA: ribonucleoside-vanadyl complexes

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

Structural Determinants of Enzymic Processivity

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

The Extent to Which Ribonucleases Cleave Ribonucleic Acid

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

A ribonuclease A variant with low catalytic activity but high cytotoxicity

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

Conformational Stability Is a Determinant of Ribonuclease A Cytotoxicity

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

The Intrinsic PkA-Values of Functional Groups in Enzymes: Improper Deductions from the Ph-Dependence of Steady-State Parameter

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

The use of pH studies to determine chemical mechanisms of enzyme-catalyzed reactions

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

A sound basis for pH-dependent kinetic studies on enzymes

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

Coulombic Forces in Protein−RNA Interactions: Binding and Cleavage by Ribonuclease A and Variants at Lys7, Arg10, and Lys66†

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

Origin of the ‘inactivation’ of ribonuclease A at low salt concentration

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

[23] The S·tag fusion system for protein purification

1 reference

https://api.crossref.org/works/10.1016%2FS0076-6879%2801%2941146-3

7 January 2021

10.1016/S0076-6879(01)41146-3

Identifiers

DOI

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11582813%20AND%20SRC:MED&resulttype=core&format=json

11 November 2019

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