(Q58039604)

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Peptide mapping and internal sequencing of proteins electroblotted from two-dimensional gels onto polyvinylidene difluoride membranes

scientific article published on 01 August 1989

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:2777983%20AND%20SRC:MED&resulttype=core&format=json

5 October 2019

Peptide mapping and internal sequencing of proteins electroblotted from two-dimensional gels onto polyvinylidene difluoride membranes. A chromatographic procedure for separating proteins from detergents (English)

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5 October 2019

Richard Simpson

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5 October 2019

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5 October 2019

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L D Ward

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5 October 2019

M P Batterham

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5 October 2019

R L Moritz

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5 October 2019

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1 August 1989

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5 October 2019

Journal of Chromatography

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476

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5 October 2019

345-361

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5 October 2019

Micropreparative procedures for high sensitivity sequencing of peptides and proteins

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Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis

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Protein-blotting on Polybrene-coated glass-fiber sheets. A basis for acid hydrolysis and gas-phase sequencing of picomole quantities of protein previously separated on sodium dodecyl sulfate/polyacrylamide gel.

1 reference

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Alterations in the phenotype of plant cells studied by NH(2)-terminal amino acid-sequence analysis of proteins electroblotted from two-dimensional gel-separated total extracts.

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7 January 2021

Applications of tandem microbore liquid chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis/electroblotting in microsequence analysis

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Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose

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Covalent immobilization of proteins for high-sensitivity sequence analysis: electroblotting onto chemically activated glass from sodium dodecyl sulfate-polyacrylamide gels

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Extended N-terminal sequencing of proteins of archaebacterial ribosomes blotted from two-dimensional gels onto glass fiber and poly(vinylidene difluoride) membrane.

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A new siliconized-glass fiber as support for protein-chemical analysis of electroblotted proteins

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Functional and structural characterization of P40, a mouse glycoprotein with T-cell growth factor activity.

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Preparative elution of proteins blotted to Immobilon membranes

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A high-performance liquid chromatography procedure for recovering subnanomole amounts of protein from SDS-gel electroeluates for gas-phase sequence analysis

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7 January 2021

Gradient LC separation of macromolecules: theory and mechanism

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Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity

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Analysis of Leaf Proteins by Two-Dimensional Gel Electrophoresis: Protease Action as Exemplified by Ribulose Bisphosphate Carboxylase/ Oxygenase Degradation and Procedure to Avoid Proteolysis during Extraction

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High resolution two-dimensional electrophoresis of basic as well as acidic proteins

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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

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Automated amino acid sequence of small peptides utilizing Polybrene

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Murine hybridoma/plasmacytoma growth factor. Complete amino-acid sequence and relation to human interleukin-6.

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7 January 2021

Inhibition of in Vitro Pollen Tube Growth by Isolated S-Glycoproteins of Nicotiana alata

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The amino-terminal sequence of the 85–90K nonhormone binding component of the molybdate-stabilized estradiol receptor from calf uterus

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Sequence analysis of the nonsteroid binding component of the calf uterine estrogen receptor.

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Coomassie blue-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for direct visualization of polypeptides during electrophoresis.

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Separation of catecholamines and their metabolites by adsorption, ion-pair and soap chromatography

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Use of microbore high-performance liquid chromatography for purifying subnanomole levels of polypeptides for microsequencing

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Complete amino acid sequence of the goose-type lysozyme from the egg white of the black swan

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Identifiers

10.1016/S0021-9673(01)93881-6

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:2777983%20AND%20SRC:MED&resulttype=core&format=json

5 October 2019

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:2777983%20AND%20SRC:MED&resulttype=core&format=json

5 October 2019

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