(Q67726976)

English

Purification and characterization of a mutant human platelet phospholipase A2 expressed in Escherichia coli. Cleavage of a fusion protein with cyanogen bromide

scientific article published on January 15, 1992

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scholarly article

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17 September 2019

Purification and characterization of a mutant human platelet phospholipase A2 expressed in Escherichia coli. Cleavage of a fusion protein with cyanogen bromide (English)

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Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:1730245%20AND%20SRC:MED&resulttype=core&format=json

17 September 2019

1 reference

10.1111/J.1432-1033.1992.TB19832.X

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10 November 2022

Escherichia coli

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P. A. Franken

1

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10 November 2022

L. Van den Berg

2

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10 November 2022

J. Huang

3

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10 November 2022

P. Gunyuzlu

4

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10 November 2022

R. B. Lugtigheid

5

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10 November 2022

H. M. Verheij

6

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10 November 2022

G. H. De Haas

7

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10 November 2022

language of work or name

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10 November 2022

publication date

1 January 1992

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17 September 2019

15 January 1992

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National Center for Biotechnology Information

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10 November 2022

full work available at URL

https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1992.tb19832.x

1 reference

10.1111/J.1432-1033.1992.TB19832.X

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10 November 2022

http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1432-1033.1992.tb19832.x/fullpdf

1 reference

10.1111/J.1432-1033.1992.TB19832.X

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10 November 2022

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Europe PubMed Central

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17 September 2019

203

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17 September 2019

1-2

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17 September 2019

89-98

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17 September 2019

pUEX, a bacterial expression vector related to pEX with universal host specificity

3

1 reference

10.1111/J.1432-1033.1992.TB19832.X

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“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

4

1 reference

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10 November 2022

Improved oligonucleotide site-directed mutagenesis using M13 vectors.

5

1 reference

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Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu

6

1 reference

10.1111/J.1432-1033.1992.TB19832.X

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A novel arachidonic acid-selective cytosolic PLA2 contains a Ca(2+)-dependent translocation domain with homology to PKC and GAP

7

1 reference

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The enzymatic synthesis of phosphatidylserine and purification by CM-cellulose column chromatography

8

1 reference

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Synthesis of lecithins by acylation of O-(sn-glycero-3-phosphoryl) choline with fatty acid anhydrides

9

1 reference

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Structure and thermodynamic properties of the complexes between phospholipase A2 and lipid micelles

10

1 reference

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10 November 2022

Expression of porcine pancreatic phospholipase A2. Generation of active enzyme by sequence-specific cleavage of a hybrid protein from Escherichia coli

11

1 reference

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10 November 2022

Competitive inhibition of lipolytic enzymes. IV. Structural details of acylamino phospholipid analogues important for the potent inhibitory effects on pancreatic phospholipase A2.

12

1 reference

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10 November 2022

A novel expression vector for high-level synthesis and secretion of foreign proteins in Escherichia coli: overproduction of bovine pancreatic phospholipase A2.

13

1 reference

10.1111/J.1432-1033.1992.TB19832.X

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10 November 2022

Purification and characterization of extracellular phospholipase A2 from human synovial fluid in rheumatoid arthritis

16

1 reference

10.1111/J.1432-1033.1992.TB19832.X

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10 November 2022

Lipid-induced aggregation of phospholipase A2: sucrose density gradient ultracentrifugation and crosslinking studies

17

1 reference

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10 November 2022

How is the level of free arachidonic acid controlled in mammalian cells?

18

1 reference

10.1111/J.1432-1033.1992.TB19832.X

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10 November 2022

The primary structure of a membrane-associated phospholipase A2 from human spleen

19

1 reference

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10 November 2022

The gapped duplex DNA approach to oligonucleotide-directed mutation construction.

21

1 reference

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10 November 2022

Function of the fully conserved residues Asp99, Tyr52 and Tyr73 in phospholipase A2.

24

1 reference

10.1111/J.1432-1033.1992.TB19832.X

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10 November 2022

Influence of size and polarity of residue 31 in porcine pancreatic phospholipase A2 on catalytic properties

25

1 reference

10.1111/J.1432-1033.1992.TB19832.X

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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

26

1 reference

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Interaction of phospholipase A2 and its zymogen with divalent metal ions.

27

1 reference

10.1111/J.1432-1033.1992.TB19832.X

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Competitive inhibition of lipolytic enzymes. I. A kinetic model applicable to water-insoluble competitive inhibitors

30

1 reference

10.1111/J.1432-1033.1992.TB19832.X

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10 November 2022

DNA sequencing with chain-terminating inhibitors

31

1 reference

10.1111/J.1432-1033.1992.TB19832.X

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10 November 2022

β-Cyanoethyl N,N-dialkylamino/N-morpholinomonochloro phosphoamidites, new phosphitylating agents facilitating ease of deprotection and work-up of synthesized oligonucleotides

34

1 reference

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Chemical and biochemical aspects of platelet activating factor: a novel class of acetylated ether-linked choline-phospholipids

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1 reference

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Acquisition of native structure from reduced Trimeresurus flavoviridis phospholipase A2

36

1 reference

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Secretion of the proenzyme and active bovine pancreatic phospholipase A2 enzyme by Saccharomyces cerevisiae: design and use of a synthetic gene.

37

1 reference

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Reversible blocking of half-cystine residues of proteins and an irreversible specific deamidation of asparagine-67 of S-sulforibonuclease under mild conditions

38

1 reference

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Interaction of phospholipase A2 with micellar interfaces. Role of the N-terminal region

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Secretion of biologically active porcine prophospholipase A2 by Saccharomyces cerevisiae. Use of the prepro sequence of the alpha-mating factor.

40

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10 November 2022

Biochimica et Biophysica Acta

41

604

191

author name string

Bosch H.

publication date

1980

1 reference

10.1111/J.1432-1033.1992.TB19832.X

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Interaction of phospholipase A2 from Naja melanoleuca snake venom with monomeric substrate analogs. Activation of the enzyme by protein-protein or lipid-protein interactions?

42

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Kinetic behavior of porcine pancreatic phospholipase A2 on zwitterionic and negatively charged single-chain substrates

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Regeneration of full enzymatic activity by reoxidation of reduced pancreatic phospholipase A2.

44

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Histidine at the active site of phospholipase A2

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1 reference

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Structure of recombinant human rheumatoid arthritic synovial fluid phospholipase A2 at 2.2 A resolution

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10.1111/J.1432-1033.1992.TB19832.X

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10 November 2022

Identifiers

10.1111/J.1432-1033.1992.TB19832.X

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:1730245%20AND%20SRC:MED&resulttype=core&format=json

17 September 2019

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:1730245%20AND%20SRC:MED&resulttype=core&format=json

17 September 2019

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