(Q67912108)

English

Biotinylated antisense methylphosphonate oligodeoxynucleotides. Inhibition of spliceosome assembly and affinity selection of U1 and U2 small nuclear RNPs

scientific article published on 01 January 1991

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scholarly article

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Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:1845976%20AND%20SRC:MED&resulttype=core&format=json

19 September 2019

Biotinylated antisense methylphosphonate oligodeoxynucleotides. Inhibition of spliceosome assembly and affinity selection of U1 and U2 small nuclear RNPs (English)

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Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:1845976%20AND%20SRC:MED&resulttype=core&format=json

19 September 2019

organic compound, having C-P covalent bound

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8 December 2022

1 reference

10.1016/S0021-9258(18)52458-3

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8 December 2022

1 reference

10.1016/S0021-9258(18)52458-3

https://api.crossref.org/works/10.1016/S0021-9258(18)52458-3

8 December 2022

author name string

J. Temsamani

1

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1845976

8 December 2022

S. Agrawal

2

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Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1845976

8 December 2022

T. Pederson

3

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8 December 2022

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publication date

1 January 1991

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19 September 2019

5 January 1991

1 reference

National Center for Biotechnology Information

PubMed publication ID

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&retmode=json&id=1845976

8 December 2022

full work available at URL

http://intl.jbc.org/cgi/content/abstract/266/1/468

online access status

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Europe PubMed Central

PubMed publication ID

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8 December 2022

https://api.elsevier.com/content/article/PII:S0021925818524583?httpAccept=text/xml

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10.1016/S0021-9258(18)52458-3

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8 December 2022

https://api.elsevier.com/content/article/PII:S0021925818524583?httpAccept=text/plain

1 reference

10.1016/S0021-9258(18)52458-3

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8 December 2022

Journal of Biological Chemistry

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Europe PubMed Central

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19 September 2019

266

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19 September 2019

1

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19 September 2019

468-472

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Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:1845976%20AND%20SRC:MED&resulttype=core&format=json

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Efficient methods for attaching non-radioactive labels to the 5' ends of synthetic oligodeoxyribonucleotides

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Site-specific excision from RNA by RNase H and mixed-phosphate-backbone oligodeoxynucleotides

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U1, U2, and U4/U6 small nuclear ribonucleoproteins are required for in vitro splicing but not polyadenylation

10

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Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein

11

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U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing

12

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Antisense probing of the human U4/U6 snRNP with biotinylated 2'-OMe RNA oligonucleotides

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Base-pairing interactions between small nuclear RNAs and nuclear RNA precursors as revealed by psoralen cross-linking in vivo

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Small Nuclear RNA U2 Is Base-Paired to Heterogeneous Nuclear RNA

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Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

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2'-O-Methyl polynucleotides as templates for cell-free amino acid incorporation

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Different small nuclear ribonucleoprotein particles are involved in different steps of splicing complex formation

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Deoxynucleoside H-Phosphonate diester intermediates in the synthesis of internucleotide phosphate analogues

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Synthesis of DNA via deoxynucleoside H-phosphonate intermediates

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Phosphoramidate analogues of DNA: synthesis and thermal stability of heteroduplexes

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RNase H cleavage of RNA hybridized to oligonucleotides containing methylphosphonate, phosphorothioate and phosphodiester bonds

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Chemica scripta

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25

280

author name string

Garegg

publication date

1985

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Synthesis and hybridization studies on two complementary nona(2'-O-methyl)ribonucleotides

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Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs

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Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for pre-mRNA splicing in vitro.

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The 5′ terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors

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Probing the structure and function of U2 snRNP with antisense oligonucleotides made of 2'-OMe RNA.

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Are snRNPs involved in splicing?

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Characterization of sequence-specific oligodeoxyribonucleoside methylphosphonates and their interaction with rabbit globin mRNA.

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Crosslinking of hnRNP proteins to pre-mRNA requires U1 and U2 snRNPs

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Effect of ionic strength on the hybridization of oligodeoxynucleotides with reduced charge due to methylphosphonate linkages to unmodified oligodeoxynucleotides containing the complementary sequence

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Highly efficient chemical synthesis of 2'-O-methyloligoribonucleotides and tetrabiotinylated derivatives; novel probes that are resistant to degradation by RNA or DNA specific nucleases.

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Identifiers

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:1845976%20AND%20SRC:MED&resulttype=core&format=json

19 September 2019

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