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English
Enhanced immunodetection of blotted house dust mite protein allergens on nitrocellulose following blocking with Tween 20
scientific article published on 01 April 1989
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scholarly article
1 reference
stated in
Europe PubMed Central
PubMed publication ID
2743967
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:2743967%20AND%20SRC:MED&resulttype=core&format=json
retrieved
4 October 2019
title
Enhanced immunodetection of blotted house dust mite protein allergens on nitrocellulose following blocking with Tween 20
(English)
1 reference
stated in
Europe PubMed Central
PubMed publication ID
2743967
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:2743967%20AND%20SRC:MED&resulttype=core&format=json
retrieved
4 October 2019
main subject
Dermatophagoides pteronyssinus
1 reference
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author
Euan Tovey
series ordinal
1
1 reference
stated in
Europe PubMed Central
PubMed publication ID
2743967
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:2743967%20AND%20SRC:MED&resulttype=core&format=json
retrieved
4 October 2019
author name string
Ford SA
series ordinal
2
1 reference
stated in
Europe PubMed Central
PubMed publication ID
2743967
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:2743967%20AND%20SRC:MED&resulttype=core&format=json
retrieved
4 October 2019
Baldo BA
series ordinal
3
1 reference
stated in
Europe PubMed Central
PubMed publication ID
2743967
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:2743967%20AND%20SRC:MED&resulttype=core&format=json
retrieved
4 October 2019
publication date
1 April 1989
1 reference
stated in
Europe PubMed Central
PubMed publication ID
2743967
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:2743967%20AND%20SRC:MED&resulttype=core&format=json
retrieved
4 October 2019
published in
Electrophoresis
1 reference
stated in
Europe PubMed Central
PubMed publication ID
2743967
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:2743967%20AND%20SRC:MED&resulttype=core&format=json
retrieved
4 October 2019
volume
10
1 reference
stated in
Europe PubMed Central
PubMed publication ID
2743967
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:2743967%20AND%20SRC:MED&resulttype=core&format=json
retrieved
4 October 2019
issue
4
1 reference
stated in
Europe PubMed Central
PubMed publication ID
2743967
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:2743967%20AND%20SRC:MED&resulttype=core&format=json
retrieved
4 October 2019
page(s)
243-249
1 reference
stated in
Europe PubMed Central
PubMed publication ID
2743967
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:2743967%20AND%20SRC:MED&resulttype=core&format=json
retrieved
4 October 2019
cites work
The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Specific detection of inactive enzyme protein after polyacrylamide gel electrophoresis by a new enzyme-immunoassay method using unspecific antiserum and partially purified active enzyme: Application to rat liver phosphodiesterase I
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Tween 20 removes antibodies and other proteins from nitrocellulose
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
The use of Tween 20 alone as a blocking agent for immunoblotting can cause artefactual results
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Different blocking agents cause variation in the immunologic detection of proteins transferred to nitrocellulose membranes
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Effects of the blocking agents bovine serum albumin and Tween 20 in different buffers on immunoblotting of brain proteins and marker proteins.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Comparison of different blocking agents and nitrocelluloses in the solid phase detection of proteins by labelled antisera and protein A
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
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The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Application to the radioimmunoassay
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Comparison by electroblotting of IgE-binding components in extracts of house dust mite bodies and spent mite culture
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Identification of orchard grass (Dactylis glomerata) pollen allergens following electrophoretic transfer to nitrocellulose.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Protein blotting on nitrocellulose: some important aspects of the resolution and detection of antigens in complex extracts.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Immunoblotting with monoclonal antibodies: importance of the blocking solution
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
On the electrotransfer of polypeptides from gels to nitrocellulose membranes
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Preparative elution of proteins from nitrocellulose membranes after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Effects of the modification of transfer buffer composition and the renaturation of proteins in gels on the recognition of proteins on Western blots by monoclonal antibodies
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Quantitative electrophoretic transfer of polypeptides from SDS polyacrylamide gels to nitrocellulose sheets: a method for their re-use in immunoautoradiographic detection of antigens.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Demonstration, by renaturation in O'Farrell gels, of heterogeneity in Dictyostelium uridine diphosphoglucose pyrophosphorylase
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1002%2FELPS.1150100405
retrieved
7 January 2021
based on heuristic
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Identifiers
DOI
10.1002/ELPS.1150100405
1 reference
stated in
Europe PubMed Central
PubMed publication ID
2743967
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:2743967%20AND%20SRC:MED&resulttype=core&format=json
retrieved
4 October 2019
PubMed publication ID
2743967
1 reference
stated in
Europe PubMed Central
PubMed publication ID
2743967
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:2743967%20AND%20SRC:MED&resulttype=core&format=json
retrieved
4 October 2019
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