(Q73258793)

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Facilitated downstream processing of a histidine-tagged protein from unclarified E. coli homogenates using immobilized metal affinity expanded-bed adsorption

scientific article published on 01 January 2000

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Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:10592518%20AND%20SRC:MED&resulttype=core&format=json

2 November 2019

Facilitated downstream processing of a histidine-tagged protein from unclarified E. coli homogenates using immobilized metal affinity expanded-bed adsorption (English)

1 reference

Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:10592518%20AND%20SRC:MED&resulttype=core&format=json

2 November 2019

Escherichia coli

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R H Clemmitt

1

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2 November 2019

H A Chase

2

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2 November 2019

publication date

1 January 2000

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Europe PubMed Central

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2 November 2019

Biotechnology and Bioengineering

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2 November 2019

67

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2 November 2019

2

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2 November 2019

206-216

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2 November 2019

Pilot scale recovery of recombinant annexin V from unclarified escherichia coli homogenate using expanded bed adsorption

1 reference

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Affinity precipitation and site-specific immobilization of proteins carrying polyhistidine tails

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Ion exchange purification of G6PDH from unclarified yeast cell homogenates using expanded bed adsorption

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21 January 2018

Development of an expanded bed technique for an affinity purification of G6PDH from unclarified yeast cell homogenates.

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Purification of proteins by adsorption chromatography in expanded beds

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The use of affinity adsorbents in expanded bed adsorption

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Improvement of downstream processing of recombinant proteins by means of genetic engineering methods

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Single-step recovery of a secreted recombinant protein by expanded bed adsorption

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21 January 2018

Surface topography of histidine residues: a facile probe by immobilized metal ion affinity chromatography

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Expanded-bed adsorption in industrial bioprocessing: recent developments

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21 January 2018

Protein selectivity with immobilized metal ion-tacn sorbents: chromatographic studies with human serum proteins and several other globular proteins

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21 January 2018

Protein interaction with immobilized metal ion affinity ligands under high ionic strength conditions

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21 January 2018

Conformational stability of pGEX-expressed Schistosoma japonicum glutathione S-transferase: a detoxification enzyme and fusion-protein affinity tag.

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21 January 2018

Unstable proteins: how to subject them to chromatographic separations for purification procedures.

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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

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21 January 2018

Affinity precipitation of enzymes

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Expanded bed affinity chromatography of dehydrogenases from bakers' yeast using dye-ligand perfluoropolymer supports

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21 January 2018

BIACORE analysis of histidine-tagged proteins using a chelating NTA sensor chip.

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Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins

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Engineering proteins to facilitate bioprocessing

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Characterisation of non-porous magnetic chelator supports and their use to recover polyhistidine-tailed T4 lysozyme from a crude E. coli extract.

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Immobilized metal ion affinity chromatography

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The saga of IMAC and MIT.

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21 January 2018

Identifiers

10.1002/(SICI)1097-0290(20000120)67:2<206::AID-BIT10>3.0.CO;2-X

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:10592518%20AND%20SRC:MED&resulttype=core&format=json

2 November 2019

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:10592518%20AND%20SRC:MED&resulttype=core&format=json

2 November 2019

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