(Q74595521)

English

Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins

scientific article published on 01 September 2001

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scholarly article

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11 November 2019

Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins (English)

1 reference

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11 November 2019

author name string

A Zhang

1

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11 November 2019

S M Gonzalez

2

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11 November 2019

E J Cantor

3

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11 November 2019

S Chong

4

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11 November 2019

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publication date

1 September 2001

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11 November 2019

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11 November 2019

275

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11 November 2019

2

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11 November 2019

241-252

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11 November 2019

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1 reference

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Green fluorescent protein as a marker for gene expression

1 reference

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7 January 2021

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Protein splicing of the Saccharomyces cerevisiae VMA intein without the endonuclease motifs

1 reference

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7 January 2021

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Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system

1 reference

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Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

1 reference

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7 January 2021

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Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step

1 reference

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7 January 2021

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Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein

1 reference

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Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein

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Improved green fluorescent protein by molecular evolution using DNA shuffling

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A comprehensive set of sequence analysis programs for the VAX

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7 January 2021

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Crystal structure of PI-SceI, a homing endonuclease with protein splicing activity

1 reference

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7 January 2021

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The in vitro ligation of bacterially expressed proteins using an intein from Methanobacterium thermoautotrophicum

1 reference

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7 January 2021

based on heuristic

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Protein trans-splicing and cyclization by a naturally split intein from the dnaE gene of Synechocystis species PCC6803

1 reference

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7 January 2021

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Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase

1 reference

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Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3 (supplement).

1 reference

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7 January 2021

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Gene fusion expression systems in Escherichia coli

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7 January 2021

based on heuristic

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Characterization of a naturally occurring trans-splicing intein from Synechocystis sp. PCC6803.

1 reference

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7 January 2021

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Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation

1 reference

https://api.crossref.org/works/10.1016%2FS0378-1119%2801%2900663-1

7 January 2021

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InBase, the Intein Database

1 reference

https://api.crossref.org/works/10.1016%2FS0378-1119%2801%2900663-1

7 January 2021

based on heuristic

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Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature

1 reference

https://api.crossref.org/works/10.1016%2FS0378-1119%2801%2900663-1

7 January 2021

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Primary structure of the Aequorea victoria green-fluorescent protein

1 reference

https://api.crossref.org/works/10.1016%2FS0378-1119%2801%2900663-1

7 January 2021

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Chromophore formation in green fluorescent protein

1 reference

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7 January 2021

based on heuristic

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Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein

1 reference

https://api.crossref.org/works/10.1016%2FS0378-1119%2801%2900663-1

7 January 2021

based on heuristic

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An alternative protein splicing mechanism for inteins lacking an N-terminal nucleophile

1 reference

https://api.crossref.org/works/10.1016%2FS0378-1119%2801%2900663-1

7 January 2021

based on heuristic

inferred from DOI database lookup

The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal intein

1 reference

https://api.crossref.org/works/10.1016%2FS0378-1119%2801%2900663-1

7 January 2021

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Rapid protein-folding assay using green fluorescent protein

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The roles of the C-terminal domain and type III domains of chitinase A1 from Bacillus circulans WL-12 in chitin degradation

1 reference

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Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803

1 reference

https://api.crossref.org/works/10.1016%2FS0378-1119%2801%2900663-1

7 January 2021

based on heuristic

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Protein trans-splicing and functional mini-inteins of a cyanobacterial dnaB intein

1 reference

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7 January 2021

based on heuristic

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Fusions to self-splicing inteins for protein purification

1 reference

https://api.crossref.org/works/10.1016%2FS0378-1119%2801%2900663-1

7 January 2021

based on heuristic

inferred from DOI database lookup

Identifiers

10.1016/S0378-1119(01)00663-1

1 reference

Europe PubMed Central

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11587851%20AND%20SRC:MED&resulttype=core&format=json

11 November 2019

1 reference

Europe PubMed Central

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11 November 2019

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