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Intein-mediated affinity-fusion purification of the Escherichia coli RecA protein
scientific article published on 01 December 2002
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scholarly article
1 reference
stated in
Europe PubMed Central
PubMed publication ID
12460773
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12460773%20AND%20SRC:MED&resulttype=core&format=json
retrieved
15 December 2019
title
Intein-mediated affinity-fusion purification of the Escherichia coli RecA protein
(English)
1 reference
stated in
Europe PubMed Central
PubMed publication ID
12460773
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12460773%20AND%20SRC:MED&resulttype=core&format=json
retrieved
15 December 2019
main subject
Escherichia coli
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author name string
Scott F Singleton
series ordinal
1
1 reference
stated in
Europe PubMed Central
PubMed publication ID
12460773
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12460773%20AND%20SRC:MED&resulttype=core&format=json
retrieved
15 December 2019
Rebecca A Simonette
series ordinal
2
1 reference
stated in
Europe PubMed Central
PubMed publication ID
12460773
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12460773%20AND%20SRC:MED&resulttype=core&format=json
retrieved
15 December 2019
Neil C Sharma
series ordinal
3
1 reference
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Europe PubMed Central
PubMed publication ID
12460773
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12460773%20AND%20SRC:MED&resulttype=core&format=json
retrieved
15 December 2019
Alberto I Roca
series ordinal
4
1 reference
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Europe PubMed Central
PubMed publication ID
12460773
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12460773%20AND%20SRC:MED&resulttype=core&format=json
retrieved
15 December 2019
publication date
1 December 2002
1 reference
stated in
Europe PubMed Central
PubMed publication ID
12460773
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12460773%20AND%20SRC:MED&resulttype=core&format=json
retrieved
15 December 2019
published in
Protein Expression and Purification
1 reference
stated in
Europe PubMed Central
PubMed publication ID
12460773
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12460773%20AND%20SRC:MED&resulttype=core&format=json
retrieved
15 December 2019
volume
26
1 reference
stated in
Europe PubMed Central
PubMed publication ID
12460773
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12460773%20AND%20SRC:MED&resulttype=core&format=json
retrieved
15 December 2019
issue
3
1 reference
stated in
Europe PubMed Central
PubMed publication ID
12460773
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12460773%20AND%20SRC:MED&resulttype=core&format=json
retrieved
15 December 2019
page(s)
476-488
1 reference
stated in
Europe PubMed Central
PubMed publication ID
12460773
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12460773%20AND%20SRC:MED&resulttype=core&format=json
retrieved
15 December 2019
cites work
Domain structure and dynamics in the helical filaments formed by RecA and Rad51 on DNA.
1 reference
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Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
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inferred from DOI database lookup
Does a stretched DNA structure dictate the helical geometry of RecA-like filaments?
1 reference
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Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
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7 January 2021
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inferred from DOI database lookup
The importance of repairing stalled replication forks
1 reference
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Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
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7 January 2021
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inferred from DOI database lookup
DNA strand exchange proteins: a biochemical and physical comparison.
1 reference
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Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
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inferred from DOI database lookup
Selective cleavage of human DNA: RecA-assisted restriction endonuclease (RARE) cleavage.
1 reference
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Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
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inferred from DOI database lookup
Molecular cloning and RARE cleavage mapping of human 2p, 6q, 8q, 12q, and 18q telomeres
1 reference
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Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
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7 January 2021
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inferred from DOI database lookup
RecA-mediated Achilles' heel cleavage
1 reference
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Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
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inferred from DOI database lookup
Flexible genetic engineering using RecA protein
1 reference
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Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
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inferred from DOI database lookup
Optical mapping of site-directed cleavages on single DNA molecules by the RecA-assisted restriction endonuclease technique
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Recognition of duplex DNA containing single-stranded regions by recA protein
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
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inferred from DOI database lookup
A Large-Scale Preparation and Some Physicochemical Properties of RecA Protein
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
DNA strand exchange promoted by RecA K72R. Two reaction phases with different Mg2+ requirements
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
recA protein from Escherichia coli. a very rapid and simple purification procedure: binding of adenosine 5'-triphosphate and adenosine 5'-diphosphate by the homogeneous protein
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
RecA protein rapidly crystallizes in the presence of spermidine: a valuable step in its purification and physical characterization
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
N-terminal 33 amino acid residues of Escherichia coli RecA protein contribute to its self-assembly
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
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inferred from DOI database lookup
Toxic mutations in the recA gene of E. coli prevent proper chromosome segregation
1 reference
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Crossref
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https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
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A genetic system yields self-cleaving inteins for bioseparations
1 reference
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Crossref
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https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
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7 January 2021
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Design and evaluation of a tryptophanless RecA protein with wild type activity
1 reference
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Crossref
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https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
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7 January 2021
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Mutational analysis of the RecJ exonuclease of Escherichia coli: identification of phosphoesterase motifs
1 reference
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Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
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7 January 2021
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Tight transcriptional control mechanism ensures stable high-level expression from T7 promoter-based expression plasmids
1 reference
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Crossref
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https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
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7 January 2021
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A novel concentration method for concentrating solutions of protein extracts based on dialysis techniques
1 reference
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Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
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7 January 2021
based on heuristic
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Continuous association of Escherichia coli single-stranded DNA binding protein with stable complexes of recA protein and single-stranded DNA.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
recA protein of Escherichia coli promotes branch migration, a kinetically distinct phase of DNA strand exchange
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Relationship of the physical and enzymatic properties of Escherichia coli recA protein to its strand exchange activity
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
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7 January 2021
based on heuristic
inferred from DOI database lookup
ATP-dependent renaturation of DNA catalyzed by the recA protein of Escherichia coli
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
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7 January 2021
based on heuristic
inferred from DOI database lookup
E. coli recA protein-directed cleavage of phage λ repressor requires polynucleotide
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Intein-mediated rapid purification of Cre recombinase
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Semisynthesis of cytotoxic proteins using a modified protein splicing element
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Optimized Single-Step Affinity Purification with a Self-Cleaving Intein Applied to Human Acidic Fibroblast Growth Factor
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Cloning, expression, and purification of the Staphylococcus simulans lysostaphin using the intein-chitin-binding domain (CBD) system
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Overexpression, Rapid Isolation, and Biochemical Characterization of Escherichia coli Single-Stranded DNA-Binding Protein
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Organization of the recA gene of Escherichia coli.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Protein splicing in trans by purified N- and C-terminal fragments of the Mycobacterium tuberculosis RecA intein
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Overexpression and purification of Helicobacter pylori flavodoxin and induction of a specific antiserum in rabbits
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
10 nm RecA protein filaments formed in the presence of Mg2+ and ATP gamma S may contain RNA
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Complexes of RecA protein in solution. A study by small angle neutron scattering
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Use of ion exchange chromatography for the study of RecA-DNA interaction
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS1046-5928%2802%2900571-5
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Identifiers
DOI
10.1016/S1046-5928(02)00571-5
1 reference
stated in
Europe PubMed Central
PubMed publication ID
12460773
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12460773%20AND%20SRC:MED&resulttype=core&format=json
retrieved
15 December 2019
PubMed publication ID
12460773
1 reference
stated in
Europe PubMed Central
PubMed publication ID
12460773
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:12460773%20AND%20SRC:MED&resulttype=core&format=json
retrieved
15 December 2019
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