(Q90291222)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:30017918%20AND%20SRC:MED&resulttype=core&format=json

12 April 2020

title

Methods for Physical Characterization of Phase-Separated Bodies and Membrane-less Organelles (English)

1 reference

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12 April 2020

author name string

Diana M Mitrea

1

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12 April 2020

Bappaditya Chandra

2

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12 April 2020

Mylene C Ferrolino

3

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12 April 2020

Eric B Gibbs

4

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12 April 2020

Michele Tolbert

5

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12 April 2020

Michael R White

6

1 reference

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12 April 2020

Richard W Kriwacki

7

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12 April 2020

publication date

24 July 2018

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Journal of Molecular Biology

12 April 2020

published in

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12 April 2020

volume

430

1 reference

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12 April 2020

issue

23

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12 April 2020

page(s)

4773-4805

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Phase separation of a protein-water mixture in cold cataract in the young rat lens

12 April 2020

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ATP depletion affects NPM translocation and exportation of rRNA from nuclei

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Three-dimensional imaging by deconvolution microscopy.

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Synthesis, processing, and transport of RNA within the three-dimensional context of the cell nucleus

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Gene ontology: tool for the unification of biology

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The dynamics of postmitotic reassembly of the nucleolus

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12 December 2020

In vivo analysis of Cajal body movement, separation, and joining in live human cells

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12 December 2020

New fluorescent probes for the measurement of cell membrane viscosity

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12 December 2020

Nucleolar components involved in ribosome biogenesis cycle between the nucleolus and nucleoplasm in interphase cells

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12 December 2020

Single-molecule fluorescence resonance energy transfer

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12 December 2020

Fluorescence in situ hybridization analysis of transcript dynamics in cells

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12 December 2020

Assignment of homology to genome sequences using a library of hidden Markov models that represent all proteins of known structure

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12 December 2020

Directed proteomic analysis of the human nucleolus

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12 December 2020

Paraspeckles: a novel nuclear domain

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12 December 2020

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12 December 2020

Cyclin-dependent kinases govern formation and maintenance of the nucleolus

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12 December 2020

Measuring diffusion in cell membranes by fluorescence correlation spectroscopy

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12 December 2020

Phase separation in polyelectrolyte solutions; theory of complex coacervation

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12 December 2020

Quantitative analysis of the fluorescence properties of intrinsically fluorescent proteins in living cells

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12 December 2020

Physical and functional interactions of the Arf tumor suppressor protein with nucleophosmin/B23

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12 December 2020

Analysis of binding reactions by fluorescence recovery after photobleaching

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12 December 2020

Intracellular macromolecular mobility measured by fluorescence recovery after photobleaching with confocal laser scanning microscopes.

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12 December 2020

Nucleolar proteome dynamics

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12 December 2020

Intracellular microrheology as a tool for the measurement of the local mechanical properties of live cells

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12 December 2020

The pairwise energy content estimated from amino acid composition discriminates between folded and intrinsically unstructured proteins.

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12 December 2020

IUPred: web server for the prediction of intrinsically unstructured regions of proteins based on estimated energy content

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12 December 2020

Probing single-molecule protein conformational dynamics

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12 December 2020

Nuclear aggresomes form by fusion of PML-associated aggregates.

1 reference

12 December 2020

The number of PML nuclear bodies increases in early S phase by a fission mechanism

1 reference

12 December 2020

The Drosophila melanogaster Cajal body

1 reference

12 December 2020

The X-ray interpretation of denaturation and the structure of the seed globulins

1 reference

12 December 2020

Nuclear bodies in the Drosophila germinal vesicle.

1 reference

12 December 2020

Bio-microrheology: a frontier in microrheology.

1 reference

12 December 2020

Spectral imaging: principles and applications.

1 reference

12 December 2020

Two-focus fluorescence correlation spectroscopy: a new tool for accurate and absolute diffusion measurements

1 reference

12 December 2020

Structural characterization of flexible proteins using small-angle X-ray scattering

1 reference

12 December 2020

A non-tumor suppressor role for basal p19ARF in maintaining nucleolar structure and function

1 reference

12 December 2020

Recent advances in fluorescent labeling techniques for fluorescence microscopy

1 reference

12 December 2020

A practical guide to single-molecule FRET

1 reference

12 December 2020

New concepts for fluorescence correlation spectroscopy on membranes

1 reference

12 December 2020

Regulation of cell division by intrinsically unstructured proteins: intrinsic flexibility, modularity, and signaling conduits

1 reference

12 December 2020

Dynamics of component exchange at PML nuclear bodies.

1 reference

12 December 2020

NOPdb: Nucleolar Proteome Database--2008 update

1 reference

12 December 2020

Phase contrast and differential interference contrast (DIC) microscopy

1 reference

12 December 2020

Thioflavin T as a molecular rotor: fluorescent properties of thioflavin T in solvents with different viscosity

1 reference

12 December 2020

Germline P granules are liquid droplets that localize by controlled dissolution/condensation

1 reference

12 December 2020

Super-resolution fluorescence microscopy

1 reference

12 December 2020

Unravelling the ultrastructure of stress granules and associated P-bodies in human cells.

1 reference

12 December 2020

Small-angle scattering for structural biology--expanding the frontier while avoiding the pitfalls.

1 reference

12 December 2020

Molecular mechanism of Thioflavin-T binding to amyloid fibrils

1 reference

12 December 2020

Phase separation of an IgG1 antibody solution under a low ionic strength condition

1 reference

12 December 2020

Small-angle X-ray and neutron scattering as a tool for structural systems biology

1 reference

12 December 2020

Fluorescent proteins and their applications in imaging living cells and tissues

1 reference

12 December 2020

1 reference

12 December 2020

Solid-state NMR spectroscopy on complex biomolecules

1 reference

12 December 2020

The nucleolus under stress

1 reference

12 December 2020

Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes

1 reference

12 December 2020

Structural analysis of macromolecular assemblies by electron microscopy

1 reference

12 December 2020

The nucleolus: structure/function relationship in RNA metabolism

1 reference

12 December 2020

Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging

1 reference

12 December 2020

Fluorescence correlation spectroscopy: past, present, future

1 reference

12 December 2020

Phase transitions in the assembly of multivalent signalling proteins

1 reference

12 December 2020

Photophysics of fluorescent probes for single-molecule biophysics and super-resolution imaging

1 reference

12 December 2020

Advanced fluorescence microscopy techniques--FRAP, FLIP, FLAP, FRET and FLIM.

1 reference

12 December 2020

Cell-free formation of RNA granules: low complexity sequence domains form dynamic fibers within hydrogels

1 reference

12 December 2020

A census of human soluble protein complexes

1 reference

12 December 2020

Simplified equation to extract diffusion coefficients from confocal FRAP data

1 reference

12 December 2020

Analysis of protein and lipid dynamics using confocal fluorescence recovery after photobleaching (FRAP).

1 reference

12 December 2020

Exclusively Heteronuclear13C-Detected Amino-Acid-Selective NMR Experiments for the Study of Intrinsically Disordered Proteins (IDPs)

1 reference

12 December 2020

Small-angle X-ray scattering on biological macromolecules and nanocomposites in solution

1 reference

12 December 2020

Single-molecule fluorescence in situ hybridization: quantitative imaging of single RNA molecules

1 reference

12 December 2020

Merging high-quality biochemical fractionation with a refined flow cytometry approach to monitor nucleocytoplasmic protein expression throughout the unperturbed mammalian cell cycle

1 reference

12 December 2020

Impact and progress in small and wide angle X-ray scattering (SAXS and WAXS).

1 reference

12 December 2020

Enzymatic labeling of proteins: techniques and approaches

1 reference

12 December 2020

Conformations of intrinsically disordered proteins are influenced by linear sequence distributions of oppositely charged residues

1 reference

12 December 2020

A nuclear F-actin scaffold stabilizes ribonucleoprotein droplets against gravity in large cells

1 reference

12 December 2020

DisMeta: a meta server for construct design and optimization

1 reference

12 December 2020

Intranuclear aggregation of mutant FUS/TLS as a molecular pathomechanism of amyotrophic lateral sclerosis

1 reference

12 December 2020

Probing the stiffness of isolated nucleoli by atomic force microscopy

1 reference

12 December 2020

Fluorescent probes for nucleic Acid visualization in fixed and live cells.

1 reference

12 December 2020

Shedding light on protein folding landscapes by single-molecule fluorescence

1 reference

12 December 2020

Structural polymorphism in the N-terminal oligomerization domain of NPM1

1 reference

12 December 2020

Single-molecule spectroscopy reveals polymer effects of disordered proteins in crowded environments

1 reference

12 December 2020

Protein cluster formation in aqueous solution in the presence of multivalent metal ions--a light scattering study

1 reference

12 December 2020

Probing nuclear pore complex architecture with proximity-dependent biotinylation.

1 reference

12 December 2020

Free mRNA in excess upon polysome dissociation is a scaffold for protein multimerization to form stress granules

1 reference

12 December 2020

Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution

1 reference

12 December 2020

Emerging applications of small angle solution scattering in structural biology

1 reference

12 December 2020

Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically disordered proteins in C. elegans

1 reference

12 December 2020

Dicer and Hsp104 function in a negative feedback loop to confer robustness to environmental stress.

1 reference

12 December 2020

Inverse size scaling of the nucleolus by a concentration-dependent phase transition

1 reference

12 December 2020

Phase transition of a disordered nuage protein generates environmentally responsive membraneless organelles

1 reference

12 December 2020

Any Way You Slice It-A Comparison of Confocal Microscopy Techniques.

1 reference

12 December 2020

Advanced ensemble modelling of flexible macromolecules using X-ray solution scattering

1 reference

12 December 2020

Labeling proteins on live mammalian cells using click chemistry.

1 reference

12 December 2020

The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics

1 reference

12 December 2020

1 reference

12 December 2020

Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells

1 reference

12 December 2020

The BioPlex Network: A Systematic Exploration of the Human Interactome

1 reference

12 December 2020

Comparative ultrastructure of CRM1-Nucleolar bodies (CNoBs), Intranucleolar bodies (INBs) and hybrid PML/p62 bodies uncovers new facets of nuclear body dynamic and diversity

1 reference

12 December 2020

Principles of protein targeting to the nucleolus

1 reference

12 December 2020

Methods for analysis of size-exclusion chromatography-small-angle X-ray scattering and reconstruction of protein scattering

1 reference

12 December 2020

Fluorescence recovery after photobleaching in material and life sciences: putting theory into practice

1 reference

12 December 2020

A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation.

1 reference

12 December 2020

A practical guide to small angle X-ray scattering (SAXS) of flexible and intrinsically disordered proteins

1 reference

12 December 2020

Sequence heuristics to encode phase behaviour in intrinsically disordered protein polymers

1 reference

12 December 2020

Phase separation by low complexity domains promotes stress granule assembly and drives pathological fibrillization

1 reference

12 December 2020

Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins

1 reference

12 December 2020

Determining composition of micron-scale protein deposits in neurodegenerative disease by spatially targeted optical microproteomics.

1 reference

12 December 2020

Residue-by-Residue View of In Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II

1 reference

12 December 2020

RNA Controls PolyQ Protein Phase Transitions.

1 reference

12 December 2020

Super-Resolution Microscopy: From Single Molecules to Supramolecular Assemblies.

1 reference

12 December 2020

Self-labelling enzymes as universal tags for fluorescence microscopy, super-resolution microscopy and electron microscopy

1 reference

12 December 2020

Phase separation in biology; functional organization of a higher order

1 reference

12 December 2020

Nucleation by rRNA Dictates the Precision of Nucleolus Assembly.

1 reference

12 December 2020

ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure.

1 reference

12 December 2020

Nucleophosmin integrates within the nucleolus via multi-modal interactions with proteins displaying R-rich linear motifs and rRNA.

1 reference

12 December 2020

Recent applications of light scattering measurement in the biological and biopharmaceutical sciences.

1 reference

12 December 2020

Reproduction of the FC/DFC units in nucleoli

1 reference

12 December 2020

Complex coacervation of supercharged proteins with polyelectrolytes.

1 reference

12 December 2020

Pharmaceutical Perspective on Opalescence and Liquid-Liquid Phase Separation in Protein Solutions

1 reference

12 December 2020

Weak and Transient Protein Interactions Determined by Solid-State NMR.

1 reference

12 December 2020

Coexisting Liquid Phases Underlie Nucleolar Subcompartments

1 reference

12 December 2020

Membraneless organelles can melt nucleic acid duplexes and act as biomolecular filters.

1 reference

12 December 2020

Compositional Control of Phase-Separated Cellular Bodies

1 reference

12 December 2020

FRAP to Characterize Molecular Diffusion and Interaction in Various Membrane Environments.

1 reference

12 December 2020

Sequence Determinants of Intracellular Phase Separation by Complex Coacervation of a Disordered Protein

1 reference

12 December 2020

Optimized cryo-focused ion beam sample preparation aimed at in situ structural studies of membrane proteins.

1 reference

12 December 2020

ALS Mutations Disrupt Phase Separation Mediated by α-Helical Structure in the TDP-43 Low-Complexity C-Terminal Domain.

1 reference

12 December 2020

Correlative Light and Electron Microscopy of Nucleolar Transcription in Saccharomyces cerevisiae.

1 reference

12 December 2020

Distinct stages in stress granule assembly and disassembly

1 reference

12 December 2020

Measuring the Viscosity of the Escherichia coli Plasma Membrane Using Molecular Rotors

1 reference

12 December 2020

C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles

1 reference

12 December 2020

Toxic PR Poly-Dipeptides Encoded by the C9orf72 Repeat Expansion Target LC Domain Polymers

1 reference

12 December 2020

Sequence-Specific Polyampholyte Phase Separation in Membraneless Organelles.

1 reference

12 December 2020

Single-molecule fluorescence resonance energy transfer in molecular biology

1 reference

12 December 2020

Expansion of the Gene Ontology knowledgebase and resources

1 reference

12 December 2020

Improved radiation dose efficiency in solution SAXS using a sheath flow sample environment

1 reference

12 December 2020

Toxic PRn poly-dipeptides encoded by the C9orf72 repeat expansion block nuclear import and export

1 reference

12 December 2020

Independent active and thermodynamic processes govern the nucleolus assembly in vivo

1 reference

12 December 2020

The Role of Dipeptide Repeats in C9ORF72-Related ALS-FTD.

1 reference

12 December 2020

In-cell NMR: a topical review

1 reference

12 December 2020

Stress-Triggered Phase Separation Is an Adaptive, Evolutionarily Tuned Response.

1 reference

12 December 2020

Integrated structural biology to unravel molecular mechanisms of protein-RNA recognition

1 reference

12 December 2020

Fluorescence Microscopy: A Concise Guide to Current Imaging Methods

1 reference

12 December 2020

Quantitative 3D structured illumination microscopy of nuclear structures.

1 reference

12 December 2020

Strategic and practical guidelines for successful structured illumination microscopy

1 reference

12 December 2020

A synergistic network of interactions promotes the formation of in vitro processing bodies and protects mRNA against decapping

1 reference

12 December 2020

Application of NMR to studies of intrinsically disordered proteins.

1 reference

12 December 2020

Direct observation of structure and dynamics during phase separation of an elastomeric protein

1 reference

12 December 2020

Dynamic light scattering: a practical guide and applications in biomedical sciences.

1 reference

12 December 2020

Architecture of the human interactome defines protein communities and disease networks

1 reference

12 December 2020

Programming molecular self-assembly of intrinsically disordered proteins containing sequences of low complexity

1 reference

12 December 2020

RNA phase transitions in repeat expansion disorders

1 reference

12 December 2020

Cross-β polymerization and hydrogel formation by low-complexity sequence proteins.

1 reference

12 December 2020

Phase separation drives heterochromatin domain formation

1 reference

12 December 2020

Liquid droplet formation by HP1α suggests a role for phase separation in heterochromatin.

1 reference

12 December 2020

An in situ USAXS-SAXS-WAXS study of precipitate size distribution evolution in a model Ni-based alloy

1 reference

12 December 2020

Elucidating the Weak Protein-Protein Interaction Mechanisms behind the Liquid-Liquid Phase Separation of a mAb Solution by Different Types of Additives

1 reference

12 December 2020

Phosphorylation of the FUS low-complexity domain disrupts phase separation, aggregation, and toxicity.

1 reference

12 December 2020

ATSAS 2.8: a comprehensive data analysis suite for small-angle scattering from macromolecular solutions

1 reference

12 December 2020

Liquid-liquid phase separation of the microtubule-binding repeats of the Alzheimer-related protein Tau.

1 reference

12 December 2020

Local Nucleation of Microtubule Bundles through Tubulin Concentration into a Condensed Tau Phase

1 reference

12 December 2020

In Situ Architecture and Cellular Interactions of PolyQ Inclusions

1 reference

12 December 2020

Structural and hydrodynamic properties of an intrinsically disordered region of a germ cell-specific protein on phase separation.

1 reference

12 December 2020

Structure of FUS Protein Fibrils and Its Relevance to Self-Assembly and Phase Separation of Low-Complexity Domains

1 reference

12 December 2020

Lysines in the RNA Polymerase II C-Terminal Domain Contribute to TAF15 Fibril Recruitment.

1 reference

12 December 2020

P-Body Purification Reveals the Condensation of Repressed mRNA Regulons

1 reference

12 December 2020

Phase behaviour of disordered proteins underlying low density and high permeability of liquid organelles.

1 reference

12 December 2020

Intrinsically disordered linkers determine the interplay between phase separation and gelation in multivalent proteins

1 reference

12 December 2020

Solution scattering approaches to dynamical ordering in biomolecular systems.

1 reference

12 December 2020

The Stress Granule Transcriptome Reveals Principles of mRNA Accumulation in Stress Granules

1 reference

12 December 2020

Quantitative analysis of multilayer organization of proteins and RNA in nuclear speckles at super resolution.

1 reference

12 December 2020

Nucleolar sub-compartments in motion during rRNA synthesis inhibition: Contraction of nucleolar condensed chromatin and gathering of fibrillar centers are concomitant

1 reference

12 December 2020

Phase Separation Behavior of Supercharged Proteins and Polyelectrolytes.

1 reference

12 December 2020

Structural Characterization of Highly Flexible Proteins by Small-Angle Scattering

1 reference

12 December 2020

A molecular engineering toolbox for the structural biologist

1 reference

12 December 2020

Phase separation of a yeast prion protein promotes cellular fitness.

1 reference

12 December 2020

Probing conformational exchange in weakly interacting, slowly exchanging protein systems via off-resonance R1ρ experiments: Application to studies of protein phase separation

1 reference

12 December 2020

Direct detection of carbon and nitrogen nuclei for high-resolution analysis of intrinsically disordered proteins using NMR spectroscopy.

1 reference

12 December 2020

Collapse Transitions of Proteins and the Interplay Among Backbone, Sidechain, and Solvent Interactions.

1 reference

12 December 2020

Mechanistic View of hnRNPA2 Low-Complexity Domain Structure, Interactions, and Phase Separation Altered by Mutation and Arginine Methylation.

1 reference

12 December 2020

Sequence determinants of protein phase behavior from a coarse-grained model.

1 reference

12 December 2020

High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies.

1 reference

12 December 2020

Pi-Pi contacts are an overlooked protein feature relevant to phase separation.

1 reference

12 December 2020

Deciphering the "Fuzzy" Interaction of FG Nucleoporins and Transport Factors Using Small-Angle Neutron Scattering.

1 reference

12 December 2020

15N detection harnesses the slow relaxation property of nitrogen: Delivering enhanced resolution for intrinsically disordered proteins.

1 reference

12 December 2020

Atomic structures of low-complexity protein segments reveal kinked β sheets that assemble networks.

1 reference

12 December 2020

Tau protein liquid-liquid phase separation can initiate tau aggregation.

1 reference

12 December 2020

Self-interaction of NPM1 modulates multiple mechanisms of liquid-liquid phase separation.

1 reference

12 December 2020

Systematic Characterization of Stress-Induced RNA Granulation

1 reference

12 December 2020

RNA buffers the phase separation behavior of prion-like RNA binding proteins.

1 reference

12 December 2020

Nucleocytoplasmic shuttling: The ins and outs of quantitative imaging

1 reference

12 December 2020

Atomic structures of TDP-43 LCD segments and insights into reversible or pathogenic aggregation

1 reference

12 December 2020

10.1016/J.JMB.2018.07.006

Identifiers

DOI

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:30017918%20AND%20SRC:MED&resulttype=core&format=json

12 April 2020

1 reference

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12 April 2020

1 reference