(Q101105118)
Statements
甘薯NBS类抗病基因类似物的分离与序列分析 (Chinese)
Isolation and Sequence Analysis of NBS-type Resistance Gene Analogues in Sweet Potato (Ipomoea batatas (L.) Lam.) (English)
陈观水/CHEN Guan-shui, ZHOU Yi-fei , LIN Sheng , PAN Da-ren*
周以飞
林生
利用已克隆植物抗病基因NBS(Nucleotide binding site)序列中的保守模体(motif)"P-loop"和"GLPL"合成简并引物,以甘薯(Ipomoea batatas)栽培品种青农2号基因组DNA为模板进行PCR扩增,通过T/A克隆、测序和序列分析,共得到15条具有连续ORF的抗病基因类似物(Resistance gene… (Chinese)
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Degenerate primers based on conserved motif (P-loop and GLPL) of the nucleotide binding site (NBS) region from the cloned plant disease resistance genes were used to isolate resistance gene analogues (RGAs) from genomic DNA of the sweet potato… (English)
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