(Q24311454)

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Protein stoichiometry of a multiprotein complex, the human spliceosomal U1 small nuclear ribonucleoprotein: absolute quantification using isotope-coded tags and mass spectrometry

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scholarly article

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Protein stoichiometry of a multiprotein complex, the human spliceosomal U1 small nuclear ribonucleoprotein: absolute quantification using isotope-coded tags and mass spectrometry (English)

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Small nuclear ribonucleoprotein polypeptide C

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GOA release 2020-03-11

ribonucleoprotein complex

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Reinhard Georg Lührmann

5

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Berthold Kastner

2

object named as

Berthold Kastner

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Thomas Fröhlich

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Thomas Fröhlich

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ORCID Public Data File 2021

author name string

Elisabeth O Hochleitner

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Alexander Schmidt

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Georg Arnold

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Friedrich Lottspeich

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language of work or name

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publication date

28 January 2005

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Journal of Biological Chemistry

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280

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2536-42

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4

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Quantitative analysis of complex protein mixtures using isotope-coded affinity tags

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Properties of 13C-Substituted Arginine in Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)

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Symmetrical dimethylation of arginine residues in spliceosomal Sm protein B/B' and the Sm-like protein LSm4, and their interaction with the SMN protein.

1 reference

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Crystal structure at 1.92 A resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin

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Arrangement of RNA and proteins in the spliceosomal U1 small nuclear ribonucleoprotein particle

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Homodimerization of the human U1 snRNP-specific protein C

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Purification of small nuclear ribonucleoprotein particles with antibodies against modified nucleosides of small nuclear RNAs

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Quantitation and facilitated de novo sequencing of proteins by isotopic N-terminal labeling of peptides with a fragmentation-directing moiety

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Absolute quantification of the G protein-coupled receptor rhodopsin by LC/MS/MS using proteolysis product peptides and synthetic peptide standards

1 reference

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The study of macromolecular complexes by quantitative proteomics

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Identifiers

10.1074/JBC.M409587200

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