Home
Random
Nearby
Log in
Settings
Donate
About Wikidata
Disclaimers
Search
(Q27026801)
Watch
English
Advances in methods for native expression and purification of RNA for structural studies
scientific article
In more languages
edit
Statements
instance of
scholarly article
1 reference
stated in
PubMed
review article
1 reference
stated in
Europe PubMed Central
title
Advances in methods for native expression and purification of RNA for structural studies
(English)
0 references
author
Robert T Batey
series ordinal
1
1 reference
stated in
Europe PubMed Central
PMC publication ID
4127115
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24607442%20AND%20SRC:MED&resulttype=core&format=json
retrieved
9 June 2020
author name string
Robert T Batey
series ordinal
1
1 reference
stated in
Crossref
language of work or name
English
1 reference
stated in
PubMed
publication date
June 2014
1 reference
stated in
PubMed
published in
Current Opinion in Structural Biology
2 references
stated in
PubMed
stated in
Europe PubMed Central
PMC publication ID
4127115
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24607442%20AND%20SRC:MED&resulttype=core&format=json
retrieved
9 June 2020
volume
26
2 references
stated in
PubMed
stated in
Europe PubMed Central
PMC publication ID
4127115
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24607442%20AND%20SRC:MED&resulttype=core&format=json
retrieved
9 June 2020
page(s)
1-8
2 references
stated in
PubMed
stated in
Europe PubMed Central
PMC publication ID
4127115
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24607442%20AND%20SRC:MED&resulttype=core&format=json
retrieved
9 June 2020
cites work
Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
21 March 2017
Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
21 March 2017
Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
21 March 2017
Human tRNALys3UUU Is Pre-Structured by Natural Modifications for Cognate and Wobble Codon Binding through Keto–Enol Tautomerism
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
21 March 2017
Crystal Structure of a Self-Spliced Group II Intron
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
21 March 2017
Identification of novel proteins associated with yeast snR30 small nucleolar RNA
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
21 March 2017
Control of gene expression by a natural metabolite-responsive ribozyme
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
21 March 2017
Streptavidin aptamers: affinity tags for the study of RNAs and ribonucleoproteins
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
21 March 2017
Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
21 March 2017
Nondenaturing purification of co-transcriptionally folded RNA avoids common folding heterogeneity
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
21 March 2017
Ribozymes, riboswitches and beyond: regulation of gene expression without proteins
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
21 March 2017
Synthesis of small RNAs using T7 RNA polymerase
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
7 April 2017
Co-expression of RNA-protein complexes in Escherichia coli and applications to RNA biology
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
RNA-protein analysis using a conditional CRISPR nuclease
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
Fluorescence imaging of cellular metabolites with RNA.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
Structural architecture of the human long non-coding RNA, steroid receptor RNA activator
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
RNAs synthesized using photocleavable biotinylated nucleotides have dramatically improved catalytic efficiency
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
Assembly of bacterial ribosomes
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
Removal of covalent heterogeneity reveals simple folding behavior for P4-P6 RNA.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
Efficient detection of RNA-protein interactions using tethered RNAs
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
Strategies in RNA crystallography.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
In vivo expression and purification of aptamer-tagged small RNA regulators
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
Unbiased RNA-protein interaction screen by quantitative proteomics.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
Determining structures of RNA aptamers and riboswitches by X-ray crystallography
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
RNA affinity tags for the rapid purification and investigation of RNAs and RNA-protein complexes
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
In vivo dynamics of RNA polymerase II transcription.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
Improved native affinity purification of RNA.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
A general method for rapid and nondenaturing purification of RNAs
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
Superior 5' homogeneity of RNA from ATP-initiated transcription under the T7 phi 2.5 promoter
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
Synthesis of adenosine derivatives as transcription initiators and preparation of 5′ fluorescein- and biotin-labeled RNA through one-step in vitro transcription
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
RNA folding: models and perspectives
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
A simple and efficient method to transcribe RNAs with reduced 3' heterogeneity
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
Sephadex-binding RNA ligands: rapid affinity purification of RNA from complex RNA mixtures
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
A simple and efficient method to reduce nontemplated nucleotide addition at the 3 terminus of RNAs transcribed by T7 RNA polymerase
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
T7 RNA polymerase produces 5' end heterogeneity during in vitro transcription from certain templates
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
Transcribing of Escherichia coli genes with mutant T7 RNA polymerases: stability of lacZ mRNA inversely correlates with polymerase speed
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
The RNA chain elongation rate in Escherichia coli depends on the growth rate
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 September 2017
Imaging bacterial protein expression using genetically encoded RNA sensors.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
31 May 2018
Affinity purification of T7 RNA transcripts with homogeneous ends using ARiBo and CRISPR tags
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
31 May 2018
RNA-based fluorescent biosensors for live cell imaging of second messengers cyclic di-GMP and cyclic AMP-GMP
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
31 May 2018
Ultraviolet shadowing of RNA can cause significant chemical damage in seconds
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
31 May 2018
Fast production of homogeneous recombinant RNA--towards large-scale production of RNA.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
31 May 2018
The ARiBo tag: a reliable tool for affinity purification of RNAs under native conditions
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
31 May 2018
Rapid, nondenaturing RNA purification using weak anion-exchange fast performance liquid chromatography
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
31 May 2018
Purification and characterization of transcribed RNAs using gel filtration chromatography.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
31 May 2018
Encoding folding paths of RNA switches
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
31 May 2018
Large-scale preparation and purification of polyacrylamide-free RNA oligonucleotides
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
31 May 2018
Keeping RNA happy.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
31 May 2018
Mutations in T7 RNA polymerase that support the proposal for a common polymerase active site structure
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
31 May 2018
Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 November 2018
Large-scale native preparation of in vitro transcribed RNA.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 November 2018
A generic protocol for the expression and purification of recombinant RNA in Escherichia coli using a tRNA scaffold.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 November 2018
Synthesis of biotin-AMP conjugate for 5' biotin labeling of RNA through one-step in vitro transcription
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 November 2018
Structural methods for studying IRES function.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 November 2018
Recombinant RNA technology: the tRNA scaffold.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 November 2018
Rapid purification of RNAs using fast performance liquid chromatography (FPLC).
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 November 2018
Functional RNA microarrays for high-throughput screening of antiprotein aptamers
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 November 2018
Aptamer-based sensor arrays for the detection and quantitation of proteins.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 November 2018
Synthesis of helix 69 of Escherichia coli 23S rRNA containing its natural modified nucleosides, m(3)Psi and Psi.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 November 2018
Productive folding to the native state by a group II intron ribozyme
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4127115
retrieved
28 November 2018
Characterization of a set of T7 RNA polymerase active site mutants
1 reference
stated in
PubMed
reference URL
https://pubmed.ncbi.nlm.nih.gov/24607442
retrieved
12 December 2020
based on heuristic
inferred from PubMed ID database lookup
Tethered function assays: an adaptable approach to study RNA regulatory proteins
1 reference
stated in
PubMed
reference URL
https://pubmed.ncbi.nlm.nih.gov/24607442
retrieved
12 December 2020
based on heuristic
inferred from PubMed ID database lookup
Selective RNase H cleavage of target RNAs from a tRNA scaffold
1 reference
stated in
PubMed
reference URL
https://pubmed.ncbi.nlm.nih.gov/24607442
retrieved
12 December 2020
based on heuristic
inferred from PubMed ID database lookup
Identifiers
DOI
10.1016/J.SBI.2014.01.014
1 reference
stated in
Europe PubMed Central
PMC publication ID
4127115
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24607442%20AND%20SRC:MED&resulttype=core&format=json
retrieved
9 June 2020
PMC publication ID
4127115
2 references
stated in
PubMed
stated in
Europe PubMed Central
PMC publication ID
4127115
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24607442%20AND%20SRC:MED&resulttype=core&format=json
retrieved
9 June 2020
PubMed publication ID
24607442
2 references
stated in
PubMed
stated in
Europe PubMed Central
PMC publication ID
4127115
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:24607442%20AND%20SRC:MED&resulttype=core&format=json
retrieved
9 June 2020
Sitelinks
Wikipedia
(0 entries)
edit
Wikibooks
(0 entries)
edit
Wikinews
(0 entries)
edit
Wikiquote
(0 entries)
edit
Wikisource
(0 entries)
edit
Wikiversity
(0 entries)
edit
Wikivoyage
(0 entries)
edit
Wiktionary
(0 entries)
edit
Multilingual sites
(0 entries)
edit
