(Q28189719)

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T7 RNA polymerase

scientific article (publication date: 2003)

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T7 RNA polymerase (English)

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Rui Sousa

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Srabani Mukherjee

2

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publication date

2003

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Progress in Molecular Biology and Translational Science

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73

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1-41

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A single side chain prevents Escherichia coli DNA polymerase I (Klenow fragment) from incorporating ribonucleotides

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Peculiar 2-aminopurine fluorescence monitors the dynamics of open complex formation by bacteriophage T7 RNA polymerase

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Kinetic and thermodynamic basis of promoter strength: multiple steps of transcription initiation by T7 RNA polymerase are modulated by the promoter sequence

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Crystal structures of the Klenow fragment of DNA polymerase I complexed with deoxynucleoside triphosphate and pyrophosphate

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Primer terminus stabilization at the phi 29 DNA polymerase active site. Mutational analysis of conserved motif KXY.

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Structural and functional insights provided by crystal structures of DNA polymerases and their substrate complexes.

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Roles of histidine 784 and tyrosine 639 in ribose discrimination by T7 RNA polymerase

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The T7 RNA polymerase intercalating hairpin is important for promoter opening during initiation but not for RNA displacement or transcription bubble stability during elongation

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T7 promoter release mediated by DNA scrunching

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Scanning mutagenesis reveals roles for helix n of the bacteriophage T7 RNA polymerase thumb subdomain in transcription complex stability, pausing, and termination

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Role of T7 RNA polymerase His784 in start site selection and initial transcription

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A novel T7 RNA polymerase autogene for efficient cytoplasmic expression of target genes

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On the evolution of the single-subunit RNA polymerases

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New RNA Polymerase from Escherichia coli infected with Bacteriophage T7

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RNAII transcribed by IPTG-induced T7 RNA polymerase is non-functional as a replication primer for ColE1-type plasmids in Escherichia coli

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Construction of bacteriophage T7 late promoters with point mutations and characterization by in vitro transcription properties

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Structure of a transcribing T7 RNA polymerase initiation complex

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Structural basis for initiation of transcription from an RNA polymerase-promoter complex

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Distinguishing between mechanisms of eukaryotic transcriptional activation with bacteriophage T7 RNA polymerase

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Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution

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Cloning and expression of the gene for bacteriophage T7 RNA polymerase

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An attempt to unify the structure of polymerases

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The stability of abortively cycling T7 RNA polymerase complexes depends upon template conformation

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Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution

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Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements

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Transcription regulation by initiating NTP concentration: rRNA synthesis in bacteria.

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Initiation, elongation, and processivity of carboxyl-terminal mutants of T7 RNA polymerase

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In vitro Synthesis of a DNA Dependent RNA Polymerase Coded on Coliphage T7 Genome

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Characterization of structural features important for T7 RNAP elongation complex stability reveals competing complex conformations and a role for the non-template strand in RNA displacement.

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A model for the mechanism of polymerase translocation

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NTP concentration effects on initial transcription by T7 RNAP indicate that translocation occurs through passive sliding and reveal that divergent promoters have distinct NTP concentration requirements for productive initiation.

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Interactions of T7 RNA polymerase with T7 late promoters measured by footprinting with methidiumpropyl-EDTA-iron(II).

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A mutant T7 RNA polymerase that is defective in RNA binding and blocked in the early stages of transcription

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Characterization of an unusual, sequence-specific termination signal for T7 RNA polymerase

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DNA polymerase beta belongs to an ancient nucleotidyltransferase superfamily

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T7 RNA polymerase elongation complex structure and movement

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Mechanisms by which T7 lysozyme specifically regulates T7 RNA polymerase during different phases of transcription.

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Misincorporation by wild-type and mutant T7 RNA polymerases: identification of interactions that reduce misincorporation rates by stabilizing the catalytically incompetent open conformation.

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Mechanism of ribose 2'-group discrimination by an RNA polymerase

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Induction of recombinant gene expression in stably transfected cell lines using attenuated vaccinia virus MVA expressing T7 RNA polymerase with a nuclear localisation signal

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Interactions of the RNA polymerase of bacteriophage T7 with its promoter during binding and initiation of transcription

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Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants

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Effects of substitutions in a conserved DX(2)GR sequence motif, found in many DNA-dependent nucleotide polymerases, on transcription by T7 RNA polymerase

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Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme

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Kinetic mechanism of GTP binding and RNA synthesis during transcription initiation by bacteriophage T7 RNA polymerase.

1 reference

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Equilibrium and stopped-flow kinetic studies of interaction between T7 RNA polymerase and its promoters measured by protein and 2-aminopurine fluorescence changes

1 reference

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Conformational coupling in DNA polymerase fidelity

1 reference

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Use of T7 RNA polymerase in an optimized Escherichia coli coupled in vitro transcription-translation system. Application in regulatory studies and expression of long transcription units.

1 reference

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inferred from DOI database lookup

Nucleotide sequence and expression of the cloned gene of bacteriophage SP6 RNA polymerase

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RNA chain initiation by Escherichia coli RNA polymerase. Structural transitions of the enzyme in early ternary complexes

1 reference

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based on heuristic

inferred from DOI database lookup

Inhibition of T7 RNA polymerase: transcription initiation and transition from initiation to elongation are inhibited by T7 lysozyme via a ternary complex with RNA polymerase and promoter DNA

1 reference

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RNA polymerase slides home: pause and termination site recognition

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Isolation and properties of transcribing ternary complexes of Escherichia coli RNA polymerase positioned at a single template base.

1 reference

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based on heuristic

inferred from DOI database lookup

Coupling of rRNA transcription and ribosomal assembly in vivo. Formation of active ribosomal subunits in Escherichia coli requires transcription of rRNA genes by host RNA polymerase which cannot be replaced by bacteriophage T7 RNA polymerase.

1 reference

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Crystal structures of open and closed forms of binary and ternary complexes of the large fragment of Thermus aquaticus DNA polymerase I: structural basis for nucleotide incorporation.

1 reference

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based on heuristic

inferred from DOI database lookup

Promoter clearance by T7 RNA polymerase. Initial bubble collapse and transcript dissociation monitored by base analog fluorescence

1 reference

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based on heuristic

inferred from DOI database lookup

The low processivity of T7 RNA polymerase over the initially transcribed sequence can limit productive initiation in vivo.

1 reference

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based on heuristic

inferred from DOI database lookup

Mutant bacteriophage T7 RNA polymerases with altered termination properties

1 reference

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based on heuristic

inferred from DOI database lookup

Pausing and termination by bacteriophage T7 RNA polymerase

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Characterization of two types of termination signal for bacteriophage T7 RNA polymerase

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Transcribing of Escherichia coli genes with mutant T7 RNA polymerases: stability of lacZ mRNA inversely correlates with polymerase speed

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Bacteriophage T7 protein kinase phosphorylates RNase E and stabilizes mRNAs synthesized by T7 RNA polymerase

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

T7 RNA polymerase does not interact with the 5'-phosphate of the initiating nucleotide.

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Processivity in early stages of transcription by T7 RNA polymerase

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Kinetic analysis of T7 RNA polymerase transcription initiation from promoters containing single-stranded regions

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Effects of Solution Conditions on the Steady-State Kinetics of Initiation of Transcription by T7 RNA Polymerase

1 reference

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based on heuristic

inferred from DOI database lookup

Yeast mitochondrial RNA polymerase is homologous to those encoded by bacteriophages T3 and T7.

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

The phage RNA polymerases are related to DNA polymerases and reverse transcriptases

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Mechanism and control of transcription initiation in prokaryotes

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Single-stranded DNA 'blue' T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering.

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Characterization of halted T7 RNA polymerase elongation complexes reveals multiple factors that contribute to stability

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

T7 lysozyme inhibits transcription by T7 RNA polymerase

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Recognition of a human arrest site is conserved between RNA polymerase II and prokaryotic RNA polymerases

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Structural transitions mediating transcription initiation by T7 RNA polymerase

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Processivity of proteolytically modified forms of T7 RNA polymerase

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Histone octamer dissociation is not required for transcript elongation through arrays of nucleosome cores by phage T7 RNA polymerase in vitro

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Asp537, Asp812 are essential and Lys631, His811 are catalytically significant in bacteriophage T7 RNA polymerase activity

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Bacteriophage T7 RNA polymerase and its active-site mutants. Kinetic, spectroscopic and calorimetric characterization

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Efficient synthesis of nucleic acids heavily modified with non-canonical ribose 2'-groups using a mutantT7 RNA polymerase (RNAP).

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Isolation and characterization of mutant bacteriophage T7 RNA polymerases

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Studies of contacts between T7 RNA polymerase and its promoter reveal features in common with multisubunit RNA polymerases

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Identification of four conserved motifs among the RNA-dependent polymerase encoding elements

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Nucleosome transcription studied in a real-time synchronous system: test of the lexosome model and direct measurement of effects due to histone octamer

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Effects of histone tail domains on the rate of transcriptional elongation through a nucleosome

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Chemical probing of RNA by nucleotide analog interference mapping

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

A nuclease-resistant protein kinase C alpha ribozyme blocks glioma cell growth

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Sequence-specific termination by T7 RNA polymerase requires formation of paused conformation prior to the point of RNA release

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Structural and mechanistic relationships between nucleic acid polymerases

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Use of T7 RNA polymerase and its mutants for incorporation of nucleoside analogs into RNA

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

A mutant T7 RNA polymerase as a DNA polymerase.

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Model for the mechanism of bacteriophage T7 RNAP transcription initiation and termination

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Crystal structure of bacteriophage T7 RNA polymerase at 3.3 A resolution

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

The thumb's knuckle. Flexibility in the thumb subdomain of T7 RNA polymerase is revealed by the structure of a chimeric T7/T3 RNA polymerase

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

A mechanism for all polymerases

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

A chemogenetic approach to RNA function/structure analysis

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Bacteriophage T7

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Transcription of Late Phage RNA by T7 RNA Polymerase

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

The specificity loop of T7 RNA polymerase interacts first with the promoter and then with the elongating transcript, suggesting a mechanism for promoter clearance

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Identification of a minimal binding element within the T7 RNA polymerase promoter

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Evidence for DNA bending at the T7 RNA polymerase promoter

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

Specificity in transcriptional regulation in the absence of specific DNA binding sites: the case of T7 lysozyme

1 reference

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7 January 2021

based on heuristic

inferred from DOI database lookup

Role of open complex instability in kinetic promoter selection by bacteriophage T7 RNA polymerase

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

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Replication-deficient vaccinia virus encoding bacteriophage T7 RNA polymerase for transient gene expression in mammalian cells

1 reference

https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

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Crystal structure of Thermus aquaticus core RNA polymerase at 3.3 A resolution.

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https://api.crossref.org/works/10.1016%2FS0079-6603%2803%2901001-8

7 January 2021

based on heuristic

inferred from DOI database lookup

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10.1016/S0079-6603(03)01001-8

1 reference

Consolidated OpenCitations Corpus – April 2017

OpenCitations bibliographic resource ID

OpenCitations bibliographic resource ID

1 reference

Consolidated OpenCitations Corpus – April 2017

OpenCitations bibliographic resource ID

PubMed publication ID

1 reference

Consolidated OpenCitations Corpus – April 2017

OpenCitations bibliographic resource ID

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