(Q28245873)

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Rapid evolution of a protein in vitro by DNA shuffling

scientific article (publication date: 4 August 1994)

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Rapid evolution of a protein in vitro by DNA shuffling (English)

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W P Stemmer

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English

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publication date

4 August 1994

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published in

Nature

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volume

370

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issue

6488

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page(s)

389-91

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An algorithm for protein engineering: simulations of recursive ensemble mutagenesis

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Identification of amino acid substitutions that alter the substrate specificity of TEM-1 beta-lactamase

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Cloning of random-sequence oligodeoxynucleotides

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Searching sequence space by definably random mutagenesis: improving the catalytic potency of an enzyme

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Randomization of genes by PCR mutagenesis

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Applied molecular evolution

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Isolation of new ribozymes from a large pool of random sequences [see comment]

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Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase

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Directed Molecular Evolution

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More extended-spectrum beta-lactamases

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Molecular evolution of ubiquitous beta-lactamases towards extended-spectrum enzymes active against newer beta-lactam antibiotics

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Phage antibodies: filamentous phage displaying antibody variable domains

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Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda

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A new revision of the sequence of plasmid pBR322

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A standard numbering scheme for the class A beta-lactamases

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(Q28245873)

Rapid evolution of a protein in vitro by DNA shuffling

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