(Q28366458)

English

Subsite differences between the active centres of papaya peptidase A and papain as revealed by affinity chromatography. Purification of papaya peptidase A by ionic-strength-dependent affinity adsorption on an immobilized peptide inhibitor of papain

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scholarly article

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Subsite differences between the active centres of papaya peptidase A and papain as revealed by affinity chromatography. Purification of papaya peptidase A by ionic-strength-dependent affinity adsorption on an immobilized peptide inhibitor of papain (English)

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author name string

P Schack

1

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N C Kaarsholm

2

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language of work or name

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publication date

1 May 1984

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Biochemical Journal

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219

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3

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727-33

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describes a project that uses

affinity chromatography

1 reference

based on heuristic

inferred from title

A kinetic and fluorimetric investigation of papain modified at tryptophan-69 and -177 by N-bromosuccinimide

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1153538

20 March 2017

Isolation of highly active papaya peptidases A and B from commercial chymopapain

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1153538

29 September 2017

Fractionation of proteolytic enzymes of dried papaya latex. Isolation and preliminary characterization of a new proteolytic enzyme

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1153538

29 September 2017

Mapping the active site of papain with the aid of peptide substrates and inhibitors

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1153538

29 September 2017

Principles of protein-protein recognition

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1153538

29 September 2017

A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1153538

29 September 2017

A marked gradation in active-centre properties in the cysteine proteinases revealed by neutral and anionic reactivity probes. Reactivity characteristics of the thiol groups of actinidin, ficin, papain and papaya peptidase A towards 4,4'-dipyridyl di

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1153538

2 June 2018

Covalent chromatography. Preparation of fully active papain from dried papaya latex

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1153538

2 June 2018

Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1153538

2 June 2018

Kinetics of papain-catalyzed hydrolysis of -N-benzoyl-L-arginine-p-nitroanilide.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1153538

2 June 2018

A necessary modification to the preparation of papain from any high-quality latex of Carica papaya and evidence for the structural integrity of the enzyme produced by traditional methods

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1153538

2 June 2018

Affinity chromatographic purification of papain

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1153538

2 June 2018

Characterization of papaya peptidase A as a cysteine proteinase of Carica papaya L. with active-centre properties that differ from those of papain by using 2,2'-dipyridyl disulphide and 4-chloro-7-nitrobenzofurazan as reactivity probes. Use of two-p

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1153538

27 November 2018

A re-evaluation of the nomenclature of the cysteine proteinases of Carica papaya and a rational basis for their identification

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1153538

27 November 2018

The Purification of Papain by Affinity Chromatography

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1153538

27 November 2018

The kinetics of the papain-catalyzed hydrolysis of esters of carbobenzoxyglycine. Evidence for an acyl-enzyme intermediate

1 reference

https://pubmed.ncbi.nlm.nih.gov/6378179

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Substrate binding by non-activated papain

1 reference

https://pubmed.ncbi.nlm.nih.gov/6378179

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

The salting-out behavior of amides and its relation to the denaturation of proteins by salts

1 reference

https://pubmed.ncbi.nlm.nih.gov/6378179

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

On the active site of proteases. 3. Mapping the active site of papain; specific peptide inhibitors of papain

1 reference

https://pubmed.ncbi.nlm.nih.gov/6378179

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

The proteolytic degradation of the B-chain of oxidized insulin by papain, chymopapain and papaya peptidase

1 reference

https://pubmed.ncbi.nlm.nih.gov/6378179

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Binding of competitive (product) inhibitors to papain in the active and inactive state

1 reference

https://pubmed.ncbi.nlm.nih.gov/6378179

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

An agarose mercurial column for the separation of mercaptopapain and nonmercaptopapain

1 reference

https://pubmed.ncbi.nlm.nih.gov/6378179

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Studies on the extended active site of papain

1 reference

https://pubmed.ncbi.nlm.nih.gov/6378179

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

A two-step procedure for purification of papain from extract of papaya latex

1 reference

https://pubmed.ncbi.nlm.nih.gov/6378179

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

On the enhanced catalytic activity of papain towards amide substrates

1 reference

https://pubmed.ncbi.nlm.nih.gov/6378179

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Resolution of thiol-containing proteins by sequential-elution covalent chromatography

1 reference

https://pubmed.ncbi.nlm.nih.gov/6378179

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

New reagents for the introduction of the thiomethyl group at sulfhydryl residues of proteins with concomitant spectrophotometric titration of the sulfhydryls: methyl 3-nitro-2-pyridyl disulfide and methyl 2-pyridyl disulfide

1 reference

https://pubmed.ncbi.nlm.nih.gov/6378179

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Characterization of Papaya Peptidase A as an Enzyme of Extreme Basicity

1 reference

https://pubmed.ncbi.nlm.nih.gov/6378179

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1042/BJ2190727

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