(Q28592095)

31 January 2017

title

Tetracysteine-tagged prion protein allows discrimination between the native and converted forms (English)

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31 January 2017

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GOA release 2020-03-11

Roman Jerala

7

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Ina Vorberg

6

object named as

Ina Vorberg

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31 January 2017

Jernej Gaspersic

1

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31 January 2017

Iva Hafner-Bratkovic

2

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31 January 2017

Michel Stephan

3

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31 January 2017

Peter Veranic

4

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31 January 2017

Mojca Bencina

5

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31 January 2017

language of work or name

English

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publication date

1 May 2010

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31 January 2017

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31 January 2017

volume

277

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31 January 2017

issue

9

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31 January 2017

page(s)

2038–2050

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Protein misfolding and aggregation: new examples in medicine and biology of the dark side of the protein world

31 January 2017

cites work

1 reference

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Curcumin binds to the alpha-helical intermediate and to the amyloid form of prion protein - a new mechanism for the inhibition of PrP(Sc) accumulation

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In vitro conversion of full-length mammalian prion protein produces amyloid form with physical properties of PrP(Sc).

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Green fluorescent protein as a marker for gene expression

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GFP-tagged prion protein is correctly localized and functionally active in the brains of transgenic mice.

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Visualization of prion infection in transgenic mice expressing green fluorescent protein-tagged prion protein.

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Soluble dimeric prion protein binds PrP(Sc) in vivo and antagonizes prion disease

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Specific covalent labeling of recombinant protein molecules inside live cells

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New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications

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Monitoring protein stability and aggregation in vivo by real-time fluorescent labeling

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Short tetracysteine tags to beta-tubulin demonstrate the significance of small labels for live cell imaging

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Dynamic fluorescent imaging of human immunodeficiency virus type 1 gag in live cells by biarsenical labeling

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A novel method of affinity-purifying proteins using a bis-arsenical fluorescein.

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Fluorescent labeling of recombinant proteins in living cells with FlAsH.

21 January 2018

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The peculiar nature of unfolding of the human prion protein

21 January 2018

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Structure of the recombinant full-length hamster prion protein PrP(29-231): the N terminus is highly flexible

21 January 2018

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NMR solution structure of the human prion protein

21 January 2018

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The Octapeptide Repeats in Mammalian Prion Protein Constitute a pH-dependent Folding and Aggregation Site

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Pathway complexity of prion protein assembly into amyloid

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A FlAsH-based FRET approach to determine G protein-coupled receptor activation in living cells

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Accumulation of FlAsH/Lumio Green in active mitochondria can be reversed by beta-mercaptoethanol for specific staining of tetracysteine-tagged proteins

21 January 2018

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Imaging of conformational changes of proteins with a new environment-sensitive fluorescent probe designed for site-specific labeling of recombinant proteins in live cells

21 January 2018

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Mammalian cell-based optimization of the biarsenical-binding tetracysteine motif for improved fluorescence and affinity

21 January 2018

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A quantitative, highly sensitive cell-based infectivity assay for mouse scrapie prions

21 January 2018

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Scrapie infection of prion protein-deficient cell line upon ectopic expression of mutant prion proteins

21 January 2018

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Multiple amino acid residues within the rabbit prion protein inhibit formation of its abnormal isoform

21 January 2018

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Insight into the PrPC-->PrPSc conversion from the structures of antibody-bound ovine prion scrapie-susceptibility variants

21 January 2018

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Evidence for assembly of prions with left-handed beta-helices into trimers

21 January 2018

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From conversion to aggregation: protofibril formation of the prion protein

21 January 2018

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The POM monoclonals: a comprehensive set of antibodies to non-overlapping prion protein epitopes

21 January 2018

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A conformational transition at the N terminus of the prion protein features in formation of the scrapie isoform

21 January 2018

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Hydrogen/deuterium exchange mass spectrometry identifies two highly protected regions in recombinant full-length prion protein amyloid fibrils

21 January 2018

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Conformational detection of prion protein with biarsenical labeling and FlAsH fluorescence

21 January 2018

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Structural studies of the scrapie prion protein by electron crystallography

21 January 2018

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Immobilized prion protein undergoes spontaneous rearrangement to a conformation having features in common with the infectious form

21 January 2018

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Effects of different experimental conditions on the PrPSc core generated by protease digestion: implications for strain typing and molecular classification of CJD.

21 January 2018

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Diagnosis of human prion disease

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Ultrasensitive detection of scrapie prion protein using seeded conversion of recombinant prion protein

21 January 2018

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Prion strain discrimination in cell culture: the cell panel assay

21 January 2018

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Semiautomated cell-free conversion of prion protein: applications for high-throughput screening of potential antiprion drugs

21 January 2018

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Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus

21 January 2018

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Overcoming interference to retroviral superinfection results in amplified expression and transmission of cloned genes

21 January 2018

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Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production

21 January 2018

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Prions prevent neuronal cell-line death.

21 January 2018

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Prion protein NMR structures of elk and of mouse/elk hybrids

21 January 2018

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Prion strain discrimination using luminescent conjugated polymers

21 January 2018

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10.1111/J.1742-4658.2010.07619.X

21 January 2018

Identifiers

DOI

1 reference

31 January 2017

1 reference