(Q30407965)

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Optimized procedure to generate heavy isotope and selenomethionine-labeled proteins for structure determination using Escherichia coli-based expression systems

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scholarly article

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Europe PubMed Central

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21 June 2017

Optimized procedure to generate heavy isotope and selenomethionine-labeled proteins for structure determination using Escherichia coli-based expression systems (English)

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Europe PubMed Central

PubMed publication ID

21 June 2017

Escherichia coli

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based on heuristic

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3

object named as

Ursula Rinas

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Europe PubMed Central

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21 June 2017

2

object named as

Manfred Nimtz

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Europe PubMed Central

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21 June 2017

author name string

Zhaopeng Li

1

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21 June 2017

language of work or name

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publication date

9 October 2011

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21 June 2017

Applied Microbiology and Biotechnology

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21 June 2017

92

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21 June 2017

4

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Europe PubMed Central

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21 June 2017

823-833

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Europe PubMed Central

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21 June 2017

Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

Preparation of selenomethionyl proteins for phase determination

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

Uniform and residue-specific 15N-labeling of proteins on a highly deuterated background

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

The use of 2H, 13C, 15N multidimensional NMR to study the structure and dynamics of proteins

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

Determination of macromolecular structures from anomalous diffraction of synchrotron radiation

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

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High-level production of uniformly ¹⁵N- and ¹³C-enriched fusion proteins in Escherichia coli.

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

Predictable deuteration of recombinant proteins expressed in Escherichia coli

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

Simple defined autoinduction medium for high-level recombinant protein production using T7-based Escherichia coli expression systems.

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

The intra- and extracellular proteome of Aspergillus niger growing on defined medium with xylose or maltose as carbon substrate

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

High yield expression and purification of isotopically labelled human endothelin-1 for use in NMR studies

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

A method for efficient isotopic labeling of recombinant proteins

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

Novel strategies to overcome expression problems encountered with toxic proteins: application to the production of Lac repressor proteins for NMR studies.

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

Optimised fermentation strategy for 13C/15N recombinant protein labelling in Escherichia coli for NMR-structure analysis

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

Preparation of isotopically labelled recombinant beta-defensin for NMR studies

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

Protein production by auto-induction in high density shaking cultures

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

Protein identification from two-dimensional gel electrophoresis analysis of Klebsiella pneumoniae by combined use of mass spectrometry data and raw genome sequences

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

The 13C chemical-shift index: a simple method for the identification of protein secondary structure using 13C chemical-shift data

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

1H, 13C and 15N random coil NMR chemical shifts of the common amino acids. I. Investigations of nearest-neighbor effects

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

Production in two-liter beverage bottles of proteins for NMR structure determination labeled with either 15N- or 13C-15N.

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

A Suite of Triple Resonance NMR Experiments for the Backbone Assignment of 15N, 13C, 2H Labeled Proteins with High Sensitivity

1 reference

https://api.crossref.org/works/10.1007%2FS00253-011-3603-X

21 January 2018

Identifiers

10.1007/S00253-011-3603-X

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Europe PubMed Central

PubMed publication ID

21 June 2017

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PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

21 June 2017

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