(Q30719492)

26 June 2017

title

Random insertion and deletion of arbitrary number of bases for codon-based random mutation of DNAs (English)

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26 June 2017

Hiroshi Murakami

1

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26 June 2017

Takahiro Hohsaka

series ordinal

2

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26 June 2017

Masahiko Sisido

series ordinal

3

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26 June 2017

publication date

1 January 2002

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26 June 2017

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26 June 2017

volume

20

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26 June 2017

issue

1

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26 June 2017

page(s)

76-81

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Rapid evolution of a protein in vitro by DNA shuffling

26 June 2017

cites work

1 reference

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7 January 2021

Designed evolution of enzymatic properties

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7 January 2021

RNA-peptide fusions for the in vitro selection of peptides and proteins

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A new method for random mutagenesis of complete genes: enzymatic generation of mutant libraries in vitro.

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7 January 2021

Randomization of genes by PCR mutagenesis

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7 January 2021

Efficient gene targeted random mutagenesis in genetically stable Escherichia coli strains

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7 January 2021

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites

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7 January 2021

Codon cassette mutagenesis: a general method to insert or replace individual codons by using universal mutagenic cassettes

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7 January 2021

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

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7 January 2021

A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions

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7 January 2021

Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function

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7 January 2021

Codon-based mutagenesis using dimer-phosphoramidites

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7 January 2021

A general strategy for random insertion and substitution mutagenesis: substoichiometric coupling of trinucleotide phosphoramidites

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7 January 2021

Combination of DMT-mononucleotide and Fmoc-trinucleotide phosphoramidites in oligonucleotide synthesis affords an automatable codon-level mutagenesis method

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7 January 2021

Crystal structure of the Aequorea victoria green fluorescent protein

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7 January 2021

A novel mutant of green fluorescent protein with enhanced sensitivity for microanalysis at 488 nm excitation

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7 January 2021

Improved green fluorescence

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7 January 2021

Improved green fluorescent protein by molecular evolution using DNA shuffling

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7 January 2021

FACS-optimized mutants of the green fluorescent protein (GFP)

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7 January 2021

Expanding the potential of restriction endonucleases: use of hapaxoterministic enzymes

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7 January 2021

Fluorescent proteins from nonbioluminescent Anthozoa species

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7 January 2021

Mutants of Discosoma red fluorescent protein with a GFP-like chromophore

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7 January 2021

Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral

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7 January 2021

Novel fluorescent protein from Discosoma coral and its mutants possesses a unique far-red fluorescence

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7 January 2021

Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function

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7 January 2021

High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis

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7 January 2021

Site-directed incorporation of fluorescent nonnatural amino acids into streptavidin for highly sensitive detection of biotin

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7 January 2021

Incorporation of nonnatural amino acids into proteins by using various four-base codons in an Escherichia coli in vitro translation system

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7 January 2021

Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification

1 reference

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7 January 2021

Cloning full-length, cap-trapper-selected cDNAs by using the single-strand linker ligation method

1 reference

https://api.crossref.org/works/10.1038%2FNBT0102-76

7 January 2021

Polymer-stimulated ligation: enhanced blunt- Or cohesive-end ligation of DNA or deoxyribooligonudcleotides by T4 DNA Ugase in polymer solutions

1 reference

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7 January 2021

Increased cloning efficiency by temperature-cycle ligation.

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7 January 2021

Identifiers

DOI

10.1038/NBT0102-76

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Dimensions Publication ID

26 June 2017

1020515695

0 references

1 reference