(Q30823110)

26 June 2017

title

A combinatorial approach to hybrid enzymes independent of DNA homology (English)

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26 June 2017

Ostermeier M

1

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26 June 2017

Shim JH

series ordinal

2

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26 June 2017

Benkovic SJ

series ordinal

3

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26 June 2017

publication date

1 December 1999

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26 June 2017

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26 June 2017

volume

17

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26 June 2017

issue

12

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26 June 2017

page(s)

1205-1209

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DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution

26 June 2017

cites work

1 reference

7 January 2021

DNA shuffling of a family of genes from diverse species accelerates directed evolution

1 reference

7 January 2021

Molecular evolution by staggered extension process (StEP) in vitro recombination

1 reference

7 January 2021

Random-priming in vitro recombination: an effective tool for directed evolution

1 reference

7 January 2021

Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation

1 reference

7 January 2021

Rapid evolution of a protein in vitro by DNA shuffling

1 reference

7 January 2021

Antibody scFv fragments without disulfide bonds made by molecular evolution

1 reference

7 January 2021

Directed evolution of a thermostable esterase.

1 reference

7 January 2021

Directed evolution converts subtilisin E into a functional equivalent of thermitase

1 reference

7 January 2021

Improved properties of FLP recombinase evolved by cycling mutagenesis

1 reference

7 January 2021

Improved green fluorescent protein by molecular evolution using DNA shuffling

1 reference

7 January 2021

Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening

1 reference

7 January 2021

A hierarchical approach to protein molecular evolution

1 reference

7 January 2021

Combinatorial protein engineering by incremental truncation

1 reference

7 January 2021

Incremental truncation as a strategy in the engineering of novel biocatalysts

1 reference

7 January 2021

Synchronous digestion of SV40 DNA by exonuclease III.

1 reference

7 January 2021

De novo purine nucleotide biosynthesis: cloning of human and avian cDNAs encoding the trifunctional glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase by functional complementatio

1 reference

7 January 2021

The human glycinamide ribonucleotide transformylase domain: purification, characterization, and kinetic mechanism

1 reference

7 January 2021

Heterologous expression and purification of active human phosphoribosylglycinamide formyltransferase as a single domain

1 reference

7 January 2021

Protein structure-based design, synthesis, and biological evaluation of 5-thia-2,6-diamino-4(3H)-oxopyrimidines: potent inhibitors of glycinamide ribonucleotide transformylase with potent cell growth inhibition

1 reference

7 January 2021

The Protein Data Bank: a computer-based archival file for macromolecular structures

1 reference

7 January 2021

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1 reference

7 January 2021

Pentapeptide scanning mutagenesis: random insertion of a variable five amino acid cassette in a target protein

1 reference

7 January 2021

Mapping catalytically important regions of an enzyme using two-codon insertion mutagenesis: a case study correlating beta-lactamase mutants with the three-dimensional structure

1 reference

7 January 2021

Creating a bifunctional protein by insertion of beta-lactamase into the maltodextrin-binding protein

1 reference

7 January 2021

Random insertion of GFP into the cAMP-dependent protein kinase regulatory subunit from Dictyostelium discoideum

1 reference

7 January 2021

A rapid screen of active site mutants in glycinamide ribonucleotide transformylase.

1 reference

7 January 2021

Structures of apo and complexed Escherichia coli glycinamide ribonucleotide transformylase

1 reference

7 January 2021

New enzyme lineages by subdomain shuffling

1 reference

7 January 2021

Assembly of an active enzyme by the linkage of two protein modules

1 reference

7 January 2021

Hybrid enzymes: manipulating enzyme design

1 reference

7 January 2021

Optimization of DNA shuffling for high fidelity recombination

1 reference

7 January 2021

Evaluation of the kinetic mechanism of Escherichia coli glycinamide ribonucleotide transformylase

1 reference

7 January 2021

Identifiers

DOI

10.1038/70754

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26 June 2017

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