(Q30830684)

13 September 2017

Generating segmental mutations in haloalkane dehalogenase: a novel part in the directed evolution toolbox (English)

1 reference

13 September 2017

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Mariël G Pikkemaat

1

1 reference

13 September 2017

Dick B Janssen

series ordinal

2

1 reference

13 September 2017

language of work or name

English

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publication date

1 April 2002

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13 September 2017

Nucleic Acids Research

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13 September 2017

30

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13 September 2017

8

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13 September 2017

E35-5

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Application of a very high-throughput digital imaging screen to evolve the enzyme galactose oxidase

13 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Analysis of the reaction mechanism and substrate specificity of haloalkane dehalogenases by sequential and structural comparisons

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

In vivo screening of haloalkane dehalogenase mutants

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Incremental truncation as a strategy in the engineering of novel biocatalysts

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

DNA shuffling of subgenomic sequences of subtilisin

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 refined at 1.15 A resolution

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Combinatorial protein engineering by incremental truncation

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Molecular evolution by staggered extension process (StEP) in vitro recombination

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

DNA shuffling of a family of genes from diverse species accelerates directed evolution

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Random-priming in vitro recombination: an effective tool for directed evolution

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Kinetic characterization and X-ray structure of a mutant of haloalkane dehalogenase with higher catalytic activity and modified substrate range

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Rapid evolution of a protein in vitro by DNA shuffling

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

The role of spontaneous cap domain mutations in haloalkane dehalogenase specificity and evolution

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Repeats of base oligomers as the primordial coding sequences of the primeval earth and their vestiges in modern genes

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Degradation of halogenated aliphatic compounds by Xanthobacter autotrophicus GJ10

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Rapid and efficient site-specific mutagenesis without phenotypic selection

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

A general method for saturation mutagenesis of cloned DNA fragments.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Crystal structure of haloalkane dehalogenase: an enzyme to detoxify halogenated alkanes

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Randomization of genes by PCR mutagenesis

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

The alpha/beta hydrolase fold

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Indicator media for microorganisms degrading chlorinated pesticides

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Bacterial mutator genes and the control of spontaneous mutation

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of epothilones

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=113230

Increased removal capacity for 1,2-dichloroethane by biological modification of the granular activated carbon process

25 November 2018

1 reference

12 December 2020

Influence of mutations of Val226 on the catalytic rate of haloalkane dehalogenase

1 reference

12 December 2020

Experiences of a large-scale application of 1,2-dichloroethane degrading microorganisms for groundwater treatment

1 reference

12 December 2020

Reaching out. Locating and lengthening the interdomain linker in AraC protein

1 reference

12 December 2020

Identifiers

DOI

10.1093/NAR/30.8.E35

1 reference

13 September 2017

1 reference

13 September 2017

PubMed publication ID

11937643

1 reference