(Q30951235)

27 June 2017

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title

Analysis of large libraries of protein mutants using flow cytometry (English)

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27 June 2017

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Georgiou G

1

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27 June 2017

publication date

1 January 2000

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Advances in Protein Chemistry

27 June 2017

published in

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27 June 2017

volume

55

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27 June 2017

page(s)

293-315

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describes a project that uses

27 June 2017

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Directed evolution of new catalytic activity using the alpha/beta-barrel scaffold

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Yeast surface display for screening combinatorial polypeptide libraries

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Deciphering the message in protein sequences: tolerance to amino acid substitutions

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

In vitro scanning saturation mutagenesis of all the specificity determining residues in an antibody binding site

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

The cystine knot of a squash-type protease inhibitor as a structural scaffold for Escherichia coli cell surface display of conformationally constrained peptides

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Evolution of a protein fold in vitro

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Antibody affinity maturation using bacterial surface display.

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Development of an optimized expression system for the screening of antibody libraries displayed on the Escherichia coli surface.

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Flow cytometric screening of cell-based libraries

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

A strategy for the isolation of catalytic activities from repertoires of enzymes displayed on phage 1 1Edited by J. Karn

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Progress and variations in two-hybrid and three-hybrid technologies

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Baculovirus surface display: construction and screening of a eukaryotic epitope library

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Technological advances in high-throughput screening

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Using an AraC-based three-hybrid system to detect biocatalysts in vivo

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Gram-positive commensal bacteria for mucosal vaccine delivery.

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Direct random mutagenesis of gene-sized DNA fragments using polymerase chain reaction

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

A microfabricated fluorescence-activated cell sorter

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Surface display of a functional single-chain Fv antibody on staphylococci.

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Antibody-ribosome-mRNA (ARM) complexes as efficient selection particles for in vitro display and evolution of antibody combining sites

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Amino acid sequence determinants of beta-lactamase structure and activity

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Surface display of Zymomonas mobilis levansucrase by using the ice-nucleation protein of Pseudomonas syringae

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

The secretion pathway of IgA protease-type proteins in gram-negative bacteria

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Rapid assay processing by integration of dual-color fluorescence cross-correlation spectroscopy: high throughput screening for enzyme activity

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Directed evolution of enzyme catalysts

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Strategies for rare cell detection and isolation

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Redesigning enzyme topology by directed evolution

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Engineered cell surfaces: fertile ground for molecular landscaping

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Genetic studies of the lac repressor. XIV. Analysis of 4000 altered Escherichia coli lac repressors reveals essential and non-essential residues, as well as "spacers" which do not require a specific sequence.

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Strategies for the in vitro evolution of protein function: enzyme evolution by random recombination of improved sequences

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

An in vivo library-versus-library selection of optimized protein-protein interactions

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Totally in vitro protein selection using mRNA-protein fusions and ribosome display

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Phage-display technology--finding a needle in a vast molecular haystack

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Exploiting recombination in single bacteria to make large phage antibody libraries

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Yeast polypeptide fusion surface display levels predict thermal stability and soluble secretion efficiency

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Biosynthetic polypeptide libraries

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Bacterial surface display: trends and progress

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Rapid evolution of a protein in vitro by DNA shuffling

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

High-throughput screening of small molecules in miniaturized mammalian cell-based assays involving post-translational modifications

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Genetic studies of the Lac repressor. XV: 4000 single amino acid substitutions and analysis of the resulting phenotypes on the basis of the protein structure

1 reference

https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

High-throughput and ultra-high-throughput screening: solution- and cell-based approaches

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Man-made cell-like compartments for molecular evolution

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Hypermutagenic PCR involving all four transitions and a sizeable proportion of transversions.

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Sequence requirements of the GPNG beta-turn of the Ecballium elaterium trypsin inhibitor II explored by combinatorial library screening

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Quantitation of transcription and clonal selection of single living cells with beta-lactamase as reporter

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

Randomization of genes by PCR mutagenesis

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https://api.crossref.org/works/10.1016%2FS0065-3233%2801%2955007-X

7 January 2021

10.1016/S0065-3233(01)55007-X

Identifiers

DOI

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27 June 2017

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