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PCR-induced sequence artifacts and bias: insights from comparison of two 16S rRNA clone libraries constructed from the same sample.
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Europe PubMed Central
PMCID
1317340
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:16332901%20AND%20SRC:MED&resulttype=core&format=json
retrieved
10 January 2020
title
PCR-induced sequence artifacts and bias: insights from comparison of two 16S rRNA clone libraries constructed from the same sample
(English)
1 reference
stated in
Europe PubMed Central
PMCID
1317340
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:16332901%20AND%20SRC:MED&resulttype=core&format=json
retrieved
10 January 2020
main subject
bias
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author
Silvia G. Acinas
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1
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Europe PubMed Central
PMCID
1317340
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:16332901%20AND%20SRC:MED&resulttype=core&format=json
retrieved
10 January 2020
Vanja Klepac-ceraj
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3
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Europe PubMed Central
PMCID
1317340
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:16332901%20AND%20SRC:MED&resulttype=core&format=json
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10 January 2020
Martin F. Polz
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4
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Europe PubMed Central
PMCID
1317340
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:16332901%20AND%20SRC:MED&resulttype=core&format=json
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10 January 2020
author name string
Ramahi Sarma-Rupavtarm
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2
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Europe PubMed Central
PMCID
1317340
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:16332901%20AND%20SRC:MED&resulttype=core&format=json
retrieved
10 January 2020
language of work or name
English
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publication date
1 December 2005
1 reference
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Europe PubMed Central
PMCID
1317340
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:16332901%20AND%20SRC:MED&resulttype=core&format=json
retrieved
10 January 2020
published in
Applied and Environmental Microbiology
1 reference
stated in
Europe PubMed Central
PMCID
1317340
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:16332901%20AND%20SRC:MED&resulttype=core&format=json
retrieved
10 January 2020
volume
71
1 reference
stated in
Europe PubMed Central
PMCID
1317340
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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:16332901%20AND%20SRC:MED&resulttype=core&format=json
retrieved
10 January 2020
page(s)
8966-8969
1 reference
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Europe PubMed Central
PMCID
1317340
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:16332901%20AND%20SRC:MED&resulttype=core&format=json
retrieved
10 January 2020
issue
12
1 reference
stated in
Europe PubMed Central
PMCID
1317340
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:16332901%20AND%20SRC:MED&resulttype=core&format=json
retrieved
10 January 2020
cites work
Extensive variation in intracellular symbiont community composition among members of a single population of the wood-boring bivalve Lyrodus pedicellatus (Bivalvia: Teredinidae).
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Genome sequence of Silicibacter pomeroyi reveals adaptations to the marine environment
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Reevaluation and reduction of a PCR bias caused by reannealing of templates.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Fine-scale phylogenetic architecture of a complex bacterial community.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Divergence and redundancy of 16S rRNA sequences in genomes with multiple rrn operons.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Environmental genome shotgun sequencing of the Sargasso Sea
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
ARB: a software environment for sequence data
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
A newly discovered Roseobacter cluster in temperate and polar oceans
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Diversity and Abundance of Uncultured Cytophaga -Like Bacteria in the Delaware Estuary
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Evaluation of PCR amplification bias by terminal restriction fragment length polymorphism analysis of small-subunit rRNA and mcrA genes by using defined template mixtures of methanogenic pure cultures and soil DNA extracts
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
SAR11 clade dominates ocean surface bacterioplankton communities.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by 'reconditioning PCR'
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Counting the uncountable: statistical approaches to estimating microbial diversity
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Optimization of annealing temperature to reduce bias caused by a primer mismatch in multitemplate PCR
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Evaluation of PCR-generated chimeras, mutations, and heteroduplexes with 16S rRNA gene-based cloning
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Microvariation artifacts introduced by PCR and cloning of closely related 16S rRNA gene sequences
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Bacterial community structure associated with a dimethylsulfoniopropionate-producing North Atlantic algal bloom
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Automated approach for ribosomal intergenic spacer analysis of microbial diversity and its application to freshwater bacterial communities.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Diversity and heterogeneity of epibiotic bacterial communities on the marine nematode Eubostrichus dianae
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Kinetic bias in estimates of coastal picoplankton community structure obtained by measurements of small-subunit rRNA gene PCR amplicon length heterogeneity
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Bias in template-to-product ratios in multitemplate PCR
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Frequency of formation of chimeric molecules as a consequence of PCR coamplification of 16S rRNA genes from mixed bacterial genomes.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
A new computational method for detection of chimeric 16S rRNA artifacts generated by PCR amplification from mixed bacterial populations.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
PCR fidelity of pfu DNA polymerase and other thermostable DNA polymerases
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Recognition of chimeric small-subunit ribosomal DNAs composed of genes from uncultivated microorganisms.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Evaluation of nearest-neighbor methods for detection of chimeric small-subunit rRNA sequences
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Chimeric cDNA clones: a novel PCR artifact
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Modeling of heteroduplex formation during PCR from mixtures of DNA templates
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
14 July 2018
Genotypic diversity within a natural coastal bacterioplankton population.
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
31 October 2018
Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
31 October 2018
An evaluation of terminal-restriction fragment length polymorphism (T-RFLP) analysis for the study of microbial community structure and dynamics
1 reference
stated in
PubMed Central
reference URL
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=1317340
retrieved
31 October 2018
The frequency of chimeric molecules as a consequence of PCR co-amplification of 16S rRNA genes from different bacterial species
1 reference
stated in
PubMed
reference URL
https://pubmed.ncbi.nlm.nih.gov/16332901
retrieved
12 December 2020
based on heuristic
inferred from PubMed ID database lookup
Identifiers
DOI
10.1128/AEM.71.12.8966-8969.2005
1 reference
stated in
Europe PubMed Central
PMCID
1317340
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:16332901%20AND%20SRC:MED&resulttype=core&format=json
retrieved
10 January 2020
PMCID
1317340
1 reference
stated in
Europe PubMed Central
PMCID
1317340
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:16332901%20AND%20SRC:MED&resulttype=core&format=json
retrieved
10 January 2020
PubMed ID
16332901
1 reference
stated in
Europe PubMed Central
PMCID
1317340
reference URL
https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:16332901%20AND%20SRC:MED&resulttype=core&format=json
retrieved
10 January 2020
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