(Q33468639)

27 July 2017

title

Validation of housekeeping genes for quantitative real-time PCR in in-vivo and in-vitro models of cerebral ischaemia (English)

1 reference

27 July 2017

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1 reference

Carme Gubern

1

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Olivia Hurtado

object named as

Olivia Hurtado

2

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Ignacio Lizasoain

object named as

Ignacio Lizasoain

María Ángeles Moro Sánchez

7

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object named as

María A Moro

6

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author name string

Rocío Rodríguez

3

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27 July 2017

Jesús R Morales

4

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27 July 2017

Víctor G Romera

5

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27 July 2017

Joaquín Serena

8

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27 July 2017

Judith Mallolas

9

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27 July 2017

publication date

16 June 2009

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27 July 2017

published in

BMC Molecular Biology

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27 July 2017

volume

10

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27 July 2017

page(s)

57

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Validation of reference genes for normalization of real-time quantitative RT-PCR data in traumatic brain injury

27 July 2017

cites work

1 reference

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Housekeeping while brain's storming Validation of normalizing factors for gene expression studies in a murine model of traumatic brain injury

21 June 2018

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Cyclooxygenase inhibition in ischemic brain injury.

21 June 2018

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Selection of reference genes for quantitative real-time PCR in a rat asphyxial cardiac arrest model

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Identification of valid reference genes for the normalization of RT qPCR gene expression data in human brain tissue

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

The genomic response of the ipsilateral and contralateral cortex to stroke in aged rats

21 June 2018

1 reference

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Evaluation and validation of candidate endogenous control genes for real-time quantitative PCR studies of breast cancer

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Evidence based selection of housekeeping genes

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

A polymorphism in the EAAT2 promoter is associated with higher glutamate concentrations and higher frequency of progressing stroke

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization

21 June 2018

1 reference

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Neuroprotection afforded by prior citicoline administration in experimental brain ischemia: effects on glutamate transport

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Therapeutic hypothermia for acute ischemic stroke: what do laboratory studies teach us?

21 June 2018

1 reference

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Ischemic tolerance and endogenous neuroprotection

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Control selection for RNA quantitation

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Housekeeping genes as internal standards: use and limits

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Focal Cerebral Ischaemia in the Rat: 1. Description of Technique and Early Neuropathological Consequences following Middle Cerebral Artery Occlusion

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Synthesis of lipoxin A4 by 5-lipoxygenase mediates PPARgamma-dependent, neuroprotective effects of rosiglitazone in experimental stroke.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Activation of liver X receptors promotes neuroprotection and reduces brain inflammation in experimental stroke.

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Housekeeping genes revisited: different expressions depending on gender, brain area and stressor

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Reference genes for normalization: a study of rat brain tissue

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Delayed post-ischemic administration of CDP-choline increases EAAT2 association to lipid rafts and affords neuroprotection in experimental stroke.

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Bilateral changes after neonatal ischemia in the P7 rat brain

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Toll-like receptor 4 is involved in brain damage and inflammation after experimental stroke.

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

The quantification of ADAMTS expression in an animal model of cerebral ischemia using real-time PCR.

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Validation of two reference genes for mRNA level studies of murine disease models in neurobiology.

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Validation of housekeeping genes for normalizing RNA expression in real-time PCR.

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

The quantification of gene expression in an animal model of brain ischaemia using TaqMan real-time RT-PCR.

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Identification of differentially expressed genes induced by transient ischemic stroke

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Alterations of Bcl-2 family proteins precede cytoskeletal proteolysis in the penumbra, but not in infarct centres following focal cerebral ischemia in mice.

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Combined oxygen and glucose deprivation in cortical cell culture: calcium-dependent and calcium-independent mechanisms of neuronal injury

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

bcl-2 is expressed in neurons that survive focal ischemia in the rat.

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2706836

Focal Cerebral Ischaemia in the Rat: 2. Regional Cerebral Blood Flow Determined by [14C]Iodoantipyrine Autoradiography following Middle Cerebral Artery Occlusion

31 October 2018

1 reference

12 December 2020

Focal Brain Ischemia in the Rat: Methods for Reproducible Neocortical Infarction Using Tandem Occlusion of the Distal Middle Cerebral and Ipsilateral Common Carotid Arteries

1 reference

12 December 2020

Effects of aging, dietary restriction and glucocorticoid treatment on housekeeping gene expression in rat cortex and hippocampus-evaluation by real time RT-PCR

1 reference

12 December 2020

The use of quantitative RT-PCR to measure mRNA expression in a rat model of focal ischemia--caspase-3 as a case study

1 reference

12 December 2020

The use of TaqMan RT-PCR assays for semiquantitative analysis of gene expression in CNS tissues and disease models

1 reference

12 December 2020

Gene expression profiling in perilesional and contralateral areas after ischemia in rat brain

1 reference

12 December 2020

Identifiers

DOI

10.1186/1471-2199-10-57

1 reference

Dimensions Publication ID

27 July 2017

0 references

1 reference

27 July 2017

1 reference