(Q33712390)

28 July 2017

1 reference

title

Improving operating performance of glucoamylase by mutagenesis (English)

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28 July 2017

Ford C

1

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28 July 2017

publication date

1 August 1999

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Current Opinion in Biotechnology

28 July 2017

published in

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28 July 2017

volume

10

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28 July 2017

issue

4

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28 July 2017

page(s)

353-357

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Kinetics, equilibria, and modeling of the formation of oligosaccharides from D-glucose with Aspergillus niger glucoamylases I and II.

28 July 2017

cites work

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Kinetics of the hydrolysis of di- and trisaccharides with Aspergillus niger glucoamylases I and II.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Refined crystal structures of glucoamylase from Aspergillus awamori var. X100

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Structure-function relationships in the catalytic and starch binding domains of glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Effect of amino acid deletions in the O-glycosylated region of Aspergillus awamori glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Expression, Glycosylation, and Secretion of an Aspergillus Glucoamylase by Saccharomyces cerevisiae

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Overexpression and characterization of Aspergillus awamori wild-type and mutant glucoamylase secreted by the methylotrophic yeast Pichia pastoris: comparison with wild-type recombinant glucoamylase produced using Saccharomyces cerevisiae and Aspergi

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Activity and thermal stability of genetically truncated forms of Aspergillus glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Adsorption to starch of a beta-galactosidase fusion protein containing the starch-binding region of Aspergillus glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Improved adsorption to starch of a beta-galactosidase fusion protein containing the starch-binding domain from Aspergillus glucoamylase.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Deletion analysis of the starch-binding domain of Aspergillus glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Thermosensitive mutants of Aspergillus awamori glucoamylase by random mutagenesis: inactivation kinetics and structural interpretation

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Substrate Binding Mechanism of Glu180→Gln, Asp176→Asn, and Wild-Type Glucoamylases fromAspergillus niger†

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Mutational Analysis of the Roles in Catalysis and Substrate Recognition of Arginines 54 and 305, Aspartic Acid 309, and Tryptophan 317 Located at Subsites 1 and 2 in Glucoamylase from Aspergillus niger

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Identification of enzyme-substrate and enzyme-product complexes in the catalytic mechanism of glucoamylase from Aspergillus awamori

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Minimizing nonproductive substrate binding: a new look at glucoamylase subsite affinities

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Protein engineering of the relative specificity of glucoamylase from Aspergillus awamori based on sequence similarities between starch-degrading enzymes

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Glucoamylase mutants in the conserved active-site segment Trp170-Tyr175 located at a distance from the site of catalysis

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Roles of the aromatic side chains in the binding of substrates, inhibitors, and cyclomalto-oligosaccharides to the glucoamylase from Aspergillus niger probed by perturbation difference spectroscopy, chemical modification, and mutagenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Cassette mutagenesis of Aspergillus awamori glucoamylase near its general acid residue to probe its catalytic and pH properties

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Some details of the reaction mechanism of glucoamylase from Aspergillus niger--kinetic and structural studies on Trp52-->Phe and Trp317-->Phe mutants

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Site-directed mutagenesis of the catalytic base glutamic acid 400 in glucoamylase from Aspergillus niger and of tyrosine 48 and glutamine 401, both hydrogen-bonded to the gamma-carboxylate group of glutamic acid 400.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Structure and energetics of the glucoamylase-isomaltose transition-state complex probed by using modeling and deoxygenated substrates coupled with site-directed mutagenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Functional and structural roles of the highly conserved Trp120 loop region of glucoamylase from Aspergillus awamori

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Reaction mechanisms of Trp120-->Phe and wild-type glucoamylases from Aspergillus niger. Interactions with maltooligodextrins and acarbose.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Site-directed mutagenesis at the active site Trp120 of Aspergillus awamori glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Catalytic mechanism of fungal glucoamylase as defined by mutagenesis of Asp176, Glu179 and Glu180 in the enzyme from Aspergillus awamori

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Functional roles and subsite locations of Leu177, Trp178 and Asn182 of Aspergillus awamori glucoamylase determined by site-directed mutagenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Kinetic identification of a hydrogen bonding pair in the glucoamylase-maltose transition state complex

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Functional roles of the invariant aspartic acid 55, tyrosine 306, and aspartic acid 309 in glucoamylase from Aspergillus awamori studied by mutagenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Catalytic mechanism of glucoamylase probed by mutagenesis in conjunction with hydrolysis of alpha-D-glucopyranosyl fluoride and maltooligosaccharides.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Structure, function and protein engineering of starch-degrading enzymes.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Increased thermostability of Asn182 --> Ala mutant Aspergillus awamori glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Substitution of asparagine residues in Aspergillus awamori glucoamylase by site-directed mutagenesis to eliminate N-glycosylation and inactivation by deamidation

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Identification and elimination by site-directed mutagenesis of thermolabile aspartyl bonds in Aspergillus awamori glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Effect of replacing helical glycine residues with alanines on reversible and irreversible stability and production of Aspergillus awamori glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Mutational modulation of substrate bond-type specificity and thermostability of glucoamylase from Aspergillus awamori by replacement with short homologue active site sequences and thiol/disulfide engineering

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Effect on thermostability and catalytic activity of introducing disulfide bonds into Aspergillus awamori glucoamylase

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Effect of introducing proline residues on the stability of Aspergillus awamori

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Stabilization of Aspergillus awamori glucoamylase by proline substitution and combining stabilizing mutations

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

A hyperthermostable pullulanase produced by an extreme thermophile, Bacillus flavocaldarius KP 1228, and evidence for the proline theory of increasing protein thermostability

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Enzymatic properties of the cysteinesulfinic acid derivative of the catalytic-base mutant Glu400-->Cys of glucoamylase from Aspergillus awamori

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Restoration of catalytic activity beyond wild-type level in glucoamylase from Aspergillus awamori by oxidation of the Glu400-->Cys catalytic-base mutant to cysteinesulfinic acid

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Protein engineering of Aspergillus awamori glucoamylase to increase its pH optimum

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Mutations to alter Aspergillus awamori glucoamylase selectivity. I. Tyr48Phe49-->Trp, Tyr116-->Trp, Tyr175-->Phe, Arg241-->Lys, Ser411-->Ala and Ser411-->Gly

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Mutations to alter Aspergillus awamori glucoamylase selectivity. II. Mutation of residues 119 and 121.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Mutations to alter Aspergillus awamori glucoamylase selectivity. III. Asn20-->Cys/Ala27-->Cys, Ala27-->Pro, Ser30-->Pro, Lys108-->Arg, Lys108-->Met, Gly137-->Ala, 311-314 Loop, Tyr312-->Trp and Ser436-->Pro

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

Mutations to alter Aspergillus awamori glucoamylase selectivity. IV. Combinations of Asn20-->Cys/Ala27-->Cys, Ser30-->Pro, Gly137-->Ala, 311-4 loop, Ser411-->Ala and Ser436-->Pro

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2899%2980064-0

7 January 2021

10.1016/S0958-1669(99)80064-0

Identifiers

DOI

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28 July 2017

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