(Q34004711)

30 July 2017

title

The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins (English)

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30 July 2017

Arne Skerra

2

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author name string

Thomas G M Schmidt

series ordinal

1

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30 July 2017

publication date

1 January 2007

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30 July 2017

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30 July 2017

volume

2

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30 July 2017

issue

6

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30 July 2017

page(s)

1528-1535

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Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins

30 July 2017

cites work

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment.

31 March 2020

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Genetically engineered avidins and streptavidins.

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One-step affinity purification of bacterially produced proteins by means of the "Strep tag" and immobilized recombinant core streptavidin.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin

31 March 2020

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https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification.

31 March 2020

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Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems

31 March 2020

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Making the most of affinity tags

31 March 2020

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https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Two-step metal affinity purification of double-tagged (NusA-His6) fusion proteins.

31 March 2020

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2007.209

A single step purification for recombinant proteins Characterization of a microtubule associated protein (MAP 2) fragment which associates with the type II cAMP‐dependent protein kinase

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https://api.crossref.org/works/10.1038%2FNPROT.2007.209

A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification

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https://api.crossref.org/works/10.1038%2FNPROT.2007.209

Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli

31 March 2020

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Assessment of prokaryotic collagen-like sequences derived from streptococcal Scl1 and Scl2 proteins as a source of recombinant GXY polymers

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Comparison of affinity tags for protein purification

31 March 2020

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Establishing a versatile fermentation and purification procedure for human proteins expressed in the yeasts Saccharomyces cerevisiae and Pichia pastoris for structural genomics

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High-throughput screening for expression of heterologous proteins in the yeast Pichia pastoris.

31 March 2020

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Exploiting features of adenovirus replication to support mammalian kinase production

31 March 2020

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Single-step Strep-tag purification for the isolation and identification of protein complexes from mammalian cells

31 March 2020

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Rapid one-step protein purification from plant material using the eight-amino acid StrepII epitope.

31 March 2020

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Structure of the periplasmic chaperone Skp suggests functional similarity with cytosolic chaperones despite differing architecture.

31 March 2020

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The 1.8-A crystal structure of human tear lipocalin reveals an extended branched cavity with capacity for multiple ligands

31 March 2020

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Structure at 2.7 A resolution of the Paracoccus denitrificans two-subunit cytochrome c oxidase complexed with an antibody FV fragment

31 March 2020

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Reversible MHC multimer staining for functional isolation of T-cell populations and effective adoptive transfer

31 March 2020

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Crystal structure and ligand-binding studies of a screened peptide complexed with streptavidin

31 March 2020

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The minimum vitamin requirements of the L and HeLa cells in tissue culture, the production of specific vitamin deficiencies, and their cure

31 March 2020

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Biotin carboxyl carrier protein co-purifies as a contaminant in core-streptavidin preparations

31 March 2020

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31 March 2020

Identifiers

DOI

10.1038/NPROT.2007.209

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Dimensions Publication ID

30 July 2017

1041454304

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