Home
Random
Nearby
Log in
Settings
Donate
About Wikidata
Disclaimers
Search
(Q34126196)
Watch
English
Protein engineering of subtilisin
scientific article
In more languages
edit
Statements
instance of
scholarly article
1 reference
stated in
Europe PubMed Central
PubMed ID
11150607
retrieved
31 July 2017
review article
1 reference
stated in
Europe PubMed Central
title
Protein engineering of subtilisin
(English)
1 reference
stated in
Europe PubMed Central
PubMed ID
11150607
retrieved
31 July 2017
author name string
P N Bryan
series ordinal
1
1 reference
stated in
Europe PubMed Central
PubMed ID
11150607
retrieved
31 July 2017
language of work or name
English
0 references
publication date
1 December 2000
1 reference
stated in
Europe PubMed Central
PubMed ID
11150607
retrieved
31 July 2017
published in
Biochimica et Biophysica Acta
1 reference
stated in
Europe PubMed Central
PubMed ID
11150607
retrieved
31 July 2017
volume
1543
1 reference
stated in
Europe PubMed Central
PubMed ID
11150607
retrieved
31 July 2017
page(s)
203-222
1 reference
stated in
Europe PubMed Central
PubMed ID
11150607
retrieved
31 July 2017
issue
2
1 reference
stated in
Europe PubMed Central
PubMed ID
11150607
retrieved
31 July 2017
cites work
Engineering subtilisin and its substrates for efficient ligation of peptide bonds in aqueous solution
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Crystal structure of calcium-independent subtilisin BPN' with restored thermal stability folded without the prodomain
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Selection for improved subtiligases by phage display
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Improvement of thermal stability of subtilisin J by changing the primary autolysis site
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Designing subtilisin BPN' to cleave substrates containing dibasic residues
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Furilisin: a variant of subtilisin BPN' engineered for cleaving tribasic substrates.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Introduction of a free cysteinyl residue at position 68 in the subtilisin Savinase, based on homology with proteinase K
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Mutational replacements in subtilisin 309. Val104 has a modulating effect on the P4 substrate preference
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Significance of hydrophobic S4-P4 interactions in subtilisin 309 from Bacillus lentus
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Kinetic studies on the peroxidase activity of selenosubtilisin
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Peroxide dependence of the semisynthetic enzyme selenosubtilisin
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Mapping the active site of papain with the aid of peptide substrates and inhibitors
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Crystal structure of the alkaline proteinase Savinase™ from Bacillus lentus at 1.4 Å resolution
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
The high-resolution X-ray crystal structure of the complex formed between subtilisin Carlsberg and eglin c, an elastase inhibitor from the leech Hirudo medicinalis. Structural analysis, subtilisin structure and interface geometry
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Incorporation of a stabilizing Ca(2+)-binding loop into subtilisin BPN'
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Subtilisin BPN' variants: increased hydrolytic activity on surface-bound substrates via decreased surface activity
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Energetics of folding subtilisin BPN'
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Site-directed mutagenesis and the role of the oxyanion hole in subtilisin
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Catalysis of a protein folding reaction: mechanistic implications of the 2.0 A structure of the subtilisin-prodomain complex
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Site-directed mutagenesis to study protein folding and stability.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Proteases of enhanced stability: characterization of a thermostable variant of subtilisin
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Probing the mechanism and improving the rate of substrate-assisted catalysis in subtilisin BPN'
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Engineering subtilisin BPN' for site-specific proteolysis.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Engineering enzyme specificity by "substrate-assisted catalysis"
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Dissecting the catalytic triad of a serine protease
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Functional interaction among catalytic residues in subtilisin BPN'.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Tuning the activity of an enzyme for unusual environments: sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
The 2 A crystal structure of subtilisin E with PMSF inhibitor
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Improvement in the alkaline stability of subtilisin using an efficient random mutagenesis and screening procedure
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
The controlled introduction of multiple negative charge at single amino acid sites in subtilisin Bacillus lentus
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
A strategy for the isolation of catalytic activities from repertoires of enzymes displayed on phage 1 1Edited by J. Karn
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Site-directed mutagenesis combined with chemical modification as a strategy for altering the specificity of the S1 and S1' pockets of subtilisin Bacillus lentus
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Probing the altered specificity and catalytic properties of mutant subtilisin chemically modified at position S156C and S166C in the S1 pocket
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Toward tailoring the specificity of the S1 pocket of subtilisin B. lentus: chemical modification of mutant enzymes as a strategy for removing specificity limitations
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Electric fields in active sites: substrate switching from null to strong fields in thiol- and selenol-subtilisins
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Folding of subtilisin BPN': characterization of a folding intermediate.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Folding of subtilisin BPN': role of the pro-sequence.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Engineering surface charges in a subtilisin: the effects on electrophoretic and ion-exchange behaviour
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Effects of engineered salt bridges on the stability of subtilisin BPN'
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Probing steric and hydrophobic effects on enzyme-substrate interactions by protein engineering.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Understanding and increasing protein stability
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Calcium-independent subtilisin by design
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Crystal structure analysis of subtilisin BPN' mutants engineered for studying thermal stability
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
The prosegment-subtilisin BPN' complex: crystal structure of a specific 'foldase'
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Stability of subtilisins and related proteinases (subtilases)
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Strategy and implementation of a system for protein engineering
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Engineered Bacillus lentus subtilisins having altered flexibility
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
A highly active and oxidation-resistant subtilisin-like enzyme produced by a combination of site-directed mutagenesis and chemical modification.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Studies of binding sites in the subtilisin from Bacillus lentus by means of site directed mutagenesis and kinetic investigations
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Interdependency of the binding subsites in subtilisin
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Extensive comparison of the substrate preferences of two subtilisins as determined with peptide substrates which are based on the principle of intramolecular quenching
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Reasoning enantioselectivity and kinetics of seleno-subtilisin from the subtilisin template
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
From detergent additive to semisynthetic peroxidase-simplified and up-scaled synthesis of seleno-subtilisin
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Chemical engineering of enzymes: altered catalytic activity, predictable selectivity and exceptional stability of the semisynthetic peroxidase seleno-subtilisin
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Semisynthetic Enzymes in Asymmetric Synthesis: Enantioselective Reduction of Racemic Hydroperoxides Catalyzed by Seleno-Subtilisin
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Renaturation of the mature subtilisin BPN' immobilized on agarose beads
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Increasing thermal stability of subtilisin from mutations suggested by strongly interacting side-chain clusters
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Binding rates, oxygen-sulfur substitution effects, and the pH dependence of chymotrypsin reactions
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Further evidence for the structure of the subtilisin propeptide and for its interactions with mature subtilisin.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
A covalently trapped folding intermediate of subtilisin E: spontaneous dimerization of a prosubtilisin E Ser49Cys mutant in vivo and its autoprocessing in vitro.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Improving the activity of immobilized subtilisin by site-specific attachment to surfaces
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Denaturation of subtilisin BPN′ and its derivatives in aqueous guanidine hydrochloride solutions
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Cloning, sequencing and expression of subtilisin Carlsberg from Bacillus licheniformis
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0 A resolution
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Effect of the weak Ca(2+)-binding site of subtilisin J by site-directed mutagenesis on heat stability.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Identification of autoproteolytic cleavage site in the Asp-49 mutant subtilisin J by site-directed mutagenesis
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Scoring functions for computational algorithms applicable to the design of spiked oligonucleotides
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Cold adaptation of a mesophilic serine protease, subtilisin, by in vitro random mutagenesis
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Breaking the low barrier hydrogen bond in a serine protease
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
A Weak Calcium Binding Site in Subtilisin BPN‘ Has a Dramatic Effect on Protein Stability
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Functional analysis of the intramolecular chaperone. Mutational hot spots in the subtilisin pro-peptide and a second-site suppressor mutation within the subtilisin molecule
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Display of active subtilisin 309 on phage: analysis of parameters influencing the selection of subtilisin variants with changed substrate specificity from libraries using phosphonylating inhibitors.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Engineering proteases with altered specificity
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Functional analysis of the propeptide of subtilisin E as an intramolecular chaperone for protein folding. Refolding and inhibitory abilities of propeptide mutants.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
The mechanism of autoprocessing of the propeptide of prosubtilisin E: intramolecular or intermolecular event?
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Binding of amino acid side-chains to S1 cavities of serine proteinases
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Interaction of subtilisin BPN' and recombinant Streptomyces subtilisin inhibitors with substituted P1 site residues.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Identification of amino acid residues responsible for the changes of absorption and fluorescence spectra on the binding of subtilisin BPN' and Streptomyces subtilisin inhibitor
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Achievement of renaturation of subtilisin BPN' by a novel procedure using organic salts and a digestible mutant of Streptomyces subtilisin inhibitor
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Structural comparison of two serine proteinase-protein inhibitor complexes: eglin-c-subtilisin Carlsberg and CI-2-subtilisin Novo
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Engineering subtilisin YaB: restriction of substrate specificity by the substitution of Gly124 and Gly151 with Ala.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Protein engineering of disulfide bonds in subtilisin BPN'.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Subtilisin from psychrophilic antarctic bacteria: characterization and site-directed mutagenesis of residues possibly involved in the adaptation to cold
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
The conversion of serine at the active site of subtilisin to cysteine: a "chemical mutation"
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
DNA shuffling of subgenomic sequences of subtilisin
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
A 13C-NMR study of the role of Asn-155 in stabilizing the oxyanion of a subtilisin tetrahedral adduct.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Unusual ligand binding at the active site domain of an engineered mutant of subtilisin BL.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Protein engineering of subtilisin BPN': enhanced stabilization through the introduction of two cysteines to form a disulfide bond
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
The engineering of binding affinity at metal ion binding sites for the stabilization of proteins: subtilisin as a test case
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Structural basis of substrate specificity in the serine proteases
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Nonessential active site residues modulate selenosubtilisin's kinetic mechanism
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Kinetics of subtilisin and thiolsubtilisin
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Comparison of the kinetic specificity of subtilisin and thiolsubtilisin toward n-alkyl p-nitrophenyl esters
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
The Reactivity of Thiol-subtilisin, an Enzyme Containing a Synthetic Functional Group*
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Chromatography and activity of thiol-subtilisin.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Free energy perturbation calculations on binding and catalysis after mutating Asn 155 in subtilisin
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Engineering a novel specificity in subtilisin BPN'
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Variants of subtilisin BPN' with altered specificity profiles
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Engineering thermostability in subtilisin BPN' by in vitro mutagenesis
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Rapid folding of calcium-free subtilisin by a stabilized pro-domain mutant
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Stabilizing the subtilisin BPN' pro-domain by phage display selection: how restrictive is the amino acid code for maximum protein stability?
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Rational modification of enzyme catalysis by engineering surface charge
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Electrostatic effects on modification of charged groups in the active site cleft of subtilisin by protein engineering
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Engineering the independent folding of the subtilisin BPN' prodomain: analysis of two-state folding versus protein stability.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Thermostable variants of subtilisin selected by temperature-gradient gel electrophoresis
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Preprosubtilisin Carlsberg processing and secretion is blocked after deletion of amino acids 97-101 in the mature part of the enzyme
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Generation of large libraries of random mutants in Bacillus subtilis by PCR-based plasmid multimerization
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Engineering new functions and altering existing functions
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Random-priming in vitro recombination: an effective tool for directed evolution
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
A pathway for conformational diversity in proteins mediated by intramolecular chaperones
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Folding mediated by an intramolecular chaperone: autoprocessing pathway of the precursor resolved via a substrate assisted catalysis mechanism.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Folding pathway mediated by an intramolecular chaperone: characterization of the structural changes in pro-subtilisin E coincident with autoprocessing
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Protein memory through altered folding mediated by intramolecular chaperones.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Mutational replacements of the amino acid residues forming the hydrophobic S4 binding pocket of subtilisin 309 from Bacillus lentus
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Stereochemical modeling of disulfide bridges. Criteria for introduction into proteins by site-directed mutagenesis
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Prediction of electrostatic effects of engineering of protein charges.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Directed evolution of a subtilisin with calcium-independent stability.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
An engineered disulfide cross-link accelerates the refolding rate of calcium-free subtilisin by 850-fold
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Catalysis of a protein folding reaction: thermodynamic and kinetic analysis of subtilisin BPN' interactions with its propeptide fragment.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Crystal structure of selenosubtilisin at 2.0-A resolution
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Engineering of a cold-adapted protease by sequential random mutagenesis and a screening system.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
A cold-adapted protease engineered by experimental evolution system
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
The Effect of Amino Acid Deletion in Subtilisin E, Based on Structural Comparison with a Microbial Alkaline Elastase, on Its Substrate Specificity and Catalysis
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Restriction of substrate specificity of subtilisin E by introduction of a side chain into a conserved glycine residue
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
The role of Pro-239 in the catalysis and heat stability of subtilisin E
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Construction of novel subtilisin E with high specificity, activity and productivity through multiple amino acid substitutions
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Random mutagenesis into the conserved Gly154 of subtilisin E: isolation and characterization of the revertant enzymes
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Thermal denaturation of streptomyces subtilisin inhibitor, subtilisin BPN', and the inhibitor-subtilisin complex.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
P1 specificity of aqualysin I (a subtilisin-type serine protease) from Thermus aquaticus YT-1, using P1-substituted derivatives of Streptomyces subtilisin inhibitor
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Engineering of S2 site of aqualysin I; alteration of P2 specificity by excluding P2 side chain
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Identification and designing of the S3 site of aqualysin I, a thermophilic subtilisin-related serine protease
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Protein engineering of the high-alkaline serine protease PB92 from Bacillus alcalophilus: functional and structural consequences of mutation at the S4 substrate binding pocket
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Tailoring the pH dependence of enzyme catalysis using protein engineering
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Length of the acyl carbonyl bond in acyl-serine proteases correlates with reactivity
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Protein engineering
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Evidence for intramolecular processing of prosubtilisin sequestered on a solid support.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Recombination and chimeragenesis by in vitro heteroduplex formation and in vivo repair
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Protein engineering of subtilisins to improve stability in detergent formulations
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Role of bound calcium ions in thermostable, proteolytic enzymes. Separation of intrinsic and calcium ion contributions to the kinetic thermal stability
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Engineering the independent folding of the subtilisin BPN' pro-domain: correlation of pro-domain stability with the rate of subtilisin folding
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Prodomain mutations at the subtilisin interface: correlation of binding energy and the rate of catalyzed folding
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Probing enzymic transition state hydrophobicities
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Additivity of mutational effects in proteins
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Recruitment of substrate-specificity properties from one enzyme into a related one by protein engineering
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Cloning, sequencing, and secretion of Bacillusamyloliquefacienssubtillisin inBacillus subtilis
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Designing substrate specificity by protein engineering of electrostatic interactions
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Details of the acyl-enzyme intermediate and the oxyanion hole in serine protease catalysis
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Enzymatic catalysts in organic synthesis
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
26 Enzymatic catalysis in organic synthesis
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Functional and nonfunctional mutations distinguished by random recombination of homologous genes
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Directed evolution converts subtilisin E into a functional equivalent of thermitase
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Molecular evolution by staggered extension process (StEP) in vitro recombination
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Strategy for the directed evolution of a peptide ligase.
1 reference
stated in
Crossref
reference URL
https://api.crossref.org/works/10.1016%2FS0167-4838%2800%2900235-1
retrieved
7 January 2021
based on heuristic
inferred from DOI database lookup
Identifiers
DOI
10.1016/S0167-4838(00)00235-1
1 reference
stated in
Europe PubMed Central
PubMed ID
11150607
retrieved
31 July 2017
PubMed ID
11150607
1 reference
stated in
Europe PubMed Central
PubMed ID
11150607
retrieved
31 July 2017
Sitelinks
Wikipedia
(0 entries)
edit
Wikibooks
(0 entries)
edit
Wikinews
(0 entries)
edit
Wikiquote
(0 entries)
edit
Wikisource
(0 entries)
edit
Wikiversity
(0 entries)
edit
Wikivoyage
(0 entries)
edit
Wiktionary
(0 entries)
edit
Multilingual sites
(0 entries)
edit