(Q34296283)

1 August 2017

title

Recent progress in intein research: from mechanism to directed evolution and applications (English)

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1 August 2017

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Gerrit Volkmann

1

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1 August 2017

Henning D Mootz

series ordinal

2

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1 August 2017

publication date

28 August 2012

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Cellular and Molecular Life Sciences

1 August 2017

published in

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1 August 2017

volume

70

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1 August 2017

issue

7

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1 August 2017

page(s)

1185-1206

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Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase

1 August 2017

cites work

1 reference

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Protein splicing of inteins and hedgehog autoproteolysis: structure, function, and evolution

21 January 2018

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Protein-splicing intein: Genetic mobility, origin, and evolution

21 January 2018

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Intein spread and extinction in evolution

21 January 2018

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Inteins: structure, function, and evolution

21 January 2018

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InBase: the Intein Database

21 January 2018

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Crystal structure of a Hedgehog autoprocessing domain: homology between Hedgehog and self-splicing proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Protein splicing and auto-cleavage of bacterial intein-like domains lacking a C'-flanking nucleophilic residue

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

New type of polyubiquitin-like genes with intein-like autoprocessing domains

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Compilation and analysis of intein sequences

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Splicing of the mycobacteriophage Bethlehem DnaB intein: identification of a new mechanistic class of inteins that contain an obligate block F nucleophile.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Split inteins as versatile tools for protein semisynthesis

21 January 2018

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Inteins, valuable genetic elements in molecular biology and biotechnology.

21 January 2018

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Biological Applications of Protein Splicing

21 January 2018

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Protein trans-splicing and its use in structural biology: opportunities and limitations.

21 January 2018

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Protein trans-splicing as a protein ligation tool to study protein structure and function.

21 January 2018

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https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Split Inteins: Nature's Protein Ligases.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Protein splicing: A versatile tool for drug discovery

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

"Splicing up" drug discovery. Cell-based expression and screening of genetically-encoded libraries of backbone-cyclized polypeptides

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

An alternative protein splicing mechanism for inteins lacking an N-terminal nucleophile

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Branched intermediate formation is the slowest step in the protein splicing reaction of the Ala1 KlbA intein from Methanococcus jannaschii

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

NMR structure of a KlbA intein precursor fromMethanococcus jannaschii

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

The Deinococcus radiodurans Snf2 intein caught in the act: detection of the Class 3 intein signature Block F branched intermediate

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Canonical protein splicing of a class 1 intein that has a class 3 noncanonical sequence motif

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Expanding the definition of class 3 inteins and their proposed phage origin

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Protein splicing and related forms of protein autoprocessing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Protein autoproteolysis: conformational strain linked to the rate of peptide cleavage by the pH dependence of the N --> O acyl shift reaction.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

A protein catalytic framework with an N-terminal nucleophile is capable of self-activation

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Conformational constraints for protein self-cleavage in the proteasome

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Identification of three core regions essential for protein splicing of the yeast Vma1 protozyme. A random mutagenesis study of the entire Vma1-derived endonuclease sequence.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Zinc inhibition of protein trans-splicing and identification of regions essential for splicing and association of a split intein*.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Structural insights into the protein splicing mechanism of PI-SceI

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Crystal structure of a mini-intein reveals a conserved catalytic module involved in side chain cyclization of asparagine during protein splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Protein-splicing reaction via a thiazolidine intermediate: crystal structure of the VMA1-derived endonuclease bearing the N and C-terminal propeptides

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Crystal structures of an intein from the split dnaE gene of Synechocystis sp. PCC6803 reveal the catalytic model without the penultimate histidine and the mechanism of zinc ion inhibition of protein splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Crystallographic and Mutational Studies of Mycobacterium tuberculosis recA Mini-inteins Suggest a Pivotal Role for a Highly Conserved Aspartate Residue

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Highly conserved histidine plays a dual catalytic role in protein splicing: a pKa shift mechanism

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Sequence requirements for splicing by the Cne PRP8 intein.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Structural and Mutational Studies of a Hyperthermophilic Intein from DNA Polymerase II of Pyrococcus abyssi

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Modular organization of inteins and C-terminal autocatalytic domains

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

The Thermococcus kodakaraensis Tko CDC21-1 intein activates its N-terminal splice junction in the absence of a conserved histidine by a compensatory mechanism

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

p K a Coupling at the Intein Active Site: Implications for the Coordination Mechanism of Protein Splicing with a Conserved Aspartate

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Probing intein-catalyzed thioester formation by unnatural amino acid substitutions in the active site

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Novel split intein for trans-splicing synthetic peptide onto C terminus of protein

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Intein lacking conserved C-terminal motif G retains controllable N-cleavage activity.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Interaction studies and alanine scanning analysis of a semi-synthetic split intein reveal thiazoline ring formation from an intermediate of the protein splicing reaction

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

3-Mercaptopropionic acid-mediated synthesis of peptide and protein thioesters.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Peptide and protein thioester synthesis via N-->S acyl transfer.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Spontaneous proton transfer to a conserved intein residue determines on-pathway protein splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Characterization of a naturally occurring trans-splicing intein from Synechocystis sp. PCC6803.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans-splicing reaction

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Branched intermediate formation stimulates peptide bond cleavage in protein splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Mechanism for intein C-terminal cleavage: a proposal from quantum mechanical calculations

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Modeling protein splicing: reaction pathway for C-terminal splice and intein scission.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Site-specific chemical modification of proteins with a prelabelled cysteine tag using the artificially split Mxe GyrA intein

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Modulation of intein activity by its neighboring extein substrates

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Evaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Ultrafast protein splicing is common among cyanobacterial split inteins: implications for protein engineering

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

NMR and Crystal Structures of the Pyrococcus horikoshii RadA Intein Guide a Strategy for Engineering a Highly Efficient and Promiscuous Intein

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Bacteriophage-based genetic system for selection of nonsplicing inteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Temperature-sensitive control of protein activity by conditionally splicing inteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Temperature-sensitive mutations made easy: generating conditional mutations by using temperature-sensitive inteins that function within different temperature ranges

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Selection and Structure of Hyperactive Inteins: Peripheral Changes Relayed to the Catalytic Center

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Minimization and stabilization of the Mycobacterium tuberculosis recA intein

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Traceless protein splicing utilizing evolved split inteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Directed evolution of ligand dependence: small-molecule-activated protein splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Highly efficient and more general cis- and trans-splicing inteins through sequential directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Backbone dynamics and global effects of an activating mutation in minimized Mtu RecA inteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Distribution of split DnaE inteins in cyanobacteria

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Trans protein splicing of cyanobacterial split inteins in endogenous and exogenous combinations.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Directed evolution of a small-molecule-triggered intein with improved splicing properties in mammalian cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Regulation of protein activity with small-molecule-controlled inteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Expressed protein ligation: a general method for protein engineering

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Semisynthesis of proteins by expressed protein ligation

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Semisynthesis of cytotoxic proteins using a modified protein splicing element

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Protein ligation: an enabling technology for the biophysical analysis of proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Development of a tandem protein trans-splicing system based on native and engineered split inteins.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Regioselective cysteine bioconjugation by appending a labeled cystein tag to a protein by using protein splicing in trans.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Expanding the scope of protein trans-splicing to fragment ligation of an integral membrane protein: towards modulation of porin-based ion channels by chemical modification

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Ligation of a synthetic peptide to the N terminus of a recombinant protein using semisynthetic protein trans-splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Protein C-terminal labeling and biotinylation using synthetic peptide and split-intein

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Site-specific two-color protein labeling for FRET studies using split inteins.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Construction of a small-molecule-integrated semisynthetic split intein for in vivo protein ligation

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Semisynthetic murine prion protein equipped with a GPI anchor mimic incorporates into cellular membranes

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Selective immobilization of proteins onto solid supports through split-intein-mediated protein trans-splicing.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Solution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

In vivo and in vitro protein ligation by naturally occurring and engineered split DnaE inteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Protein semi-synthesis in living cells.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

In-cell fluorescence activation and labeling of proteins mediated by FRET-quenched split inteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Adding new chemistries to the genetic code

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Bioluminescent imaging of MAPK function with intein-mediated reporter gene assay.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Detection of apoptosis using cyclic luciferase in living mammals

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Development of a novel DnaE intein-based assay for quantitative analysis of G-protein-coupled receptor internalization.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Split-intein mediated re-assembly of genetically encoded Ca(2+) indicators

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

A novel hybrid seed system for plants.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Intein-mediated protein assembly in transgenic wheat: production of active barnase and acetolactate synthase from split genes

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Protein trans-splicing in transgenic plant chloroplast: reconstruction of herbicide resistance from split genes

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Control of transcription factor activity and osteoblast differentiation in mammalian cells using an evolved small-molecule-dependent intein

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Protein splicing triggered by a small molecule

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Conditional protein splicing: a new tool to control protein structure and function in vitro and in vivo.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Activation of an autoregulated protein kinase by conditional protein splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Post-translational enzyme activation in an animal via optimized conditional protein splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

An intein-cassette integration approach used for the generation of a split TEV protease activated by conditional protein splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Photomodulation of protein trans-splicing through backbone photocaging of the DnaE split intein

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Activation of protein splicing by protease- or light-triggered O to N acyl migration

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Engineering artificially split inteins for applications in protein chemistry: biochemical characterization of the split Ssp DnaB intein and comparison to the split Sce VMA intein

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Kinetic control of one-pot trans-splicing reactions by using a wild-type and designed split intein

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Structure of catalytically competent intein caught in a redox trap with functional and evolutionary implications

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Protein splicing mechanisms and applications

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Kinetic analysis of the individual steps of protein splicing for the Pyrococcus abyssi PolII intein

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Protein splicing in cis and in trans

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Highly efficient proteintrans-splicing by a naturally split DnaE intein fromNostoc punctiforme

21 January 2018

1 reference

https://api.crossref.org/works/10.1007%2FS00018-012-1120-4

Characteristics of protein splicing in trans mediated by a semisynthetic split intein

21 January 2018

1 reference

12 December 2020

Protein splicing: estimation of the rate of O-N and S-N acyl rearrangements, the last step of the splicing process

1 reference

12 December 2020

The mechanism of protein splicing and its modulation by mutation

1 reference

12 December 2020

Optimized Single-Step Affinity Purification with a Self-Cleaving Intein Applied to Human Acidic Fibroblast Growth Factor

1 reference

12 December 2020

NMR observation of selected segments in a larger protein: central-segment isotope labeling through intein-mediated ligation

1 reference

12 December 2020

Protein immobilization on liposomes and lipid-coated nanoparticles by protein trans-splicing

1 reference

12 December 2020

Succinimide formation from aspartyl and asparaginyl peptides as a model for the spontaneous degradation of proteins

1 reference

12 December 2020

Protein Splicing: Characterization of the Aminosuccinimide Residue at the Carboxyl Terminus of the Excised Intervening Sequence

1 reference

12 December 2020

Photocontrol of protein activity mediated by the cleavage reaction of a split intein

1 reference

12 December 2020

Protein trans-splicing and cyclization by a naturally split intein from the dnaE gene of Synechocystis species PCC6803

1 reference

12 December 2020

Activation of protein splicing with light in yeast

1 reference

12 December 2020

A genetic system yields self-cleaving inteins for bioseparations

1 reference

12 December 2020

Development of a positive genetic selection system for inhibition of protein splicing using mycobacterial inteins in Escherichia coli DNA gyrase subunit A

1 reference

12 December 2020

Protein trans-splicing and functional mini-inteins of a cyanobacterial dnaB intein

1 reference

12 December 2020

Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein

1 reference

12 December 2020

Purification of recombinant proteins from E. coli by engineered inteins.

1 reference

12 December 2020

Protein splicing: an analysis of the branched intermediate and its resolution by succinimide formation

1 reference

12 December 2020

Molecular structure of a gene, VMA1, encoding the catalytic subunit of H(+)-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae

1 reference

12 December 2020

10.1007/S00018-012-1120-4

Identifiers

DOI

1 reference

1 August 2017

1 reference