(Q34490107)

2 August 2017

Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS). (English)

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2 August 2017

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Rajeshwary Ghosh

2

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Sue C Bodine

object named as

Sue C Bodine

5

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author name string

Jennifer E Gilda

1

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2 August 2017

Jenice X Cheah

3

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2 August 2017

Toni M West

4

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2 August 2017

Aldrin V Gomes

6

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2 August 2017

language of work or name

English

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publication date

19 August 2015

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2 August 2017

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2 August 2017

10

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2 August 2017

e0135392

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2 August 2017

8

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Creative Commons Attribution 4.0 International

2 August 2017

start time

19 August 2015

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April 2022 Public Data File from Crossref

copyright status

copyrighted

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cites work

Recombinant renewable polyclonal antibodies

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

The necessity of and strategies for improving confidence in the accuracy of western blots

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

Specificity of anti-tau antibodies when analyzing mice models of Alzheimer's disease: problems and solutions

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

Validating Antibodies to the Cannabinoid CB2 Receptor: Antibody Sensitivity Is Not Evidence of Antibody Specificity.

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

Total protein analysis as a reliable loading control for quantitative fluorescent Western blotting

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

Lack of specificity shown by P2Y6 receptor antibodies.

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

Stain-Free total protein staining is a superior loading control to β-actin for Western blots

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

A defined methodology for reliable quantification of Western blot data

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

Hindlimb unloading results in increased predisposition to cardiac arrhythmias and alters left ventricular connexin 43 expression

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

Lack of specificity of commercial antibodies leads to misidentification of angiotensin type 1 receptor protein

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

Six commercially available angiotensin II AT1 receptor antibodies are non-specific

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

Benefits and pitfalls of secondary antibodies: why choosing the right secondary is of primary importance

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

Noncovalent dimerization of ubiquitin

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

Commercially available antibodies against human and murine histamine H₄-receptor lack specificity

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

Interferon-stimulated gene 15 and the protein ISGylation system

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

An assessment of histone-modification antibody quality

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

Efficient protection and isolation of ubiquitylated proteins using tandem ubiquitin-binding entities

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

Quantifying Western blots: pitfalls of densitometry.

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

A genecentric Human Protein Atlas for expression profiles based on antibodies

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

The use of total protein stains as loading controls: an alternative to high-abundance single-protein controls in semi-quantitative immunoblotting

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

Rapamycin inhibits the growth and muscle-sparing effects of clenbuterol

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications

6 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4545415

Specificity evaluation of antibodies against human β3-adrenoceptors.

6 July 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0135392

MUC expression in hyperplastic and serrated colonic polyps: lack of specificity of MUC6.

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0135392

Several commercially available anti-CCR5 monoclonal antibodies lack specificity and should be used with caution.

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0135392

Reproducibility: Standardize antibodies used in research.

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0135392

Proteomic analysis of hearts from Akita mice suggests that increases in soluble epoxide hydrolase and antioxidative programming are key changes in early stages of diabetic cardiomyopathy.

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0135392

Western blotting using in-gel protein labeling as a normalization control: stain-free technology.

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0135392

GABARAPL1 antibodies: target one protein, get one free!

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0135392

Important considerations for protein analyses using antibody based techniques: down-sizing Western blotting up-sizes outcomes

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0135392

Are genuine changes in protein expression being overlooked? Reassessing Western blotting.

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0135392

Integrative analysis of the ubiquitin proteome isolated using Tandem Ubiquitin Binding Entities (TUBEs)

21 January 2018

1 reference

https://api.crossref.org/works/10.1371%2FJOURNAL.PONE.0135392

Detergent affects silver sequencing

21 January 2018

1 reference

12 December 2020

Finding the right antibody for the job

1 reference

12 December 2020

Chronic hindlimb suspension unloading markedly decreases turnover rates of skeletal and cardiac muscle proteins and adipose tissue triglycerides

1 reference

12 December 2020

Beta-actin is not a reliable loading control in Western blot analysis

1 reference

12 December 2020

GAPDH and β-actin protein decreases with aging, making Stain-Free technology a superior loading control in Western blotting of human skeletal muscle

1 reference

12 December 2020

10.1371/JOURNAL.PONE.0135392

Identifiers

DOI

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2 August 2017

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2 August 2017

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