(Q34587280)

3 August 2017

A new method to customize protein expression vectors for fast, efficient and background free parallel cloning (English)

1 reference

3 August 2017

Hüseyin Besir

2

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author name string

Judith Scholz

series ordinal

1

1 reference

3 August 2017

Claudia Strasser

series ordinal

3

1 reference

3 August 2017

Sabine Suppmann

series ordinal

4

1 reference

3 August 2017

14 February 2013

1 reference

3 August 2017

1 reference

3 August 2017

13

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3 August 2017

12

1 reference

https://scigraph.springernature.com/pub.10.1186/1472-6750-13-12

3 August 2017

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cites work

ORF-selector ESPRIT: a second generation library screen for soluble protein expression employing precise open reading frame selection.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3598636

Simplified, enhanced protein purification using an inducible, autoprocessing enzyme tag

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3598636

A rapid method for titrating baculovirus stocks using the Sf-9 Easy Titer cell line

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3598636

Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3598636

The titerless infected-cells preservation and scale-up (TIPS) method for large-scale production of NO-sensitive human soluble guanylate cyclase (sGC) from insect cells infected with recombinant baculovirus

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3598636

Reflections on more than 10 years of TGE approaches

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3598636

Mechanism of homologous recombination from the RecA-ssDNA/dsDNA structures

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3598636

A versatile ligation-independent cloning method suitable for high-throughput expression screening applications

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3598636

In vivo and in vitro protein solubility assays using split GFP.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3598636

Generation of a multimeric form of CD40L with potent immunostimulatory activity using streptavidin as a chaperon

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3598636

Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3598636

Protein production by auto-induction in high density shaking cultures

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3598636

A basis for SUMO protease specificity provided by analysis of human Senp2 and a Senp2-SUMO complex

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3598636

High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3598636

Expression of a recombinant form of the V antigen of Yersinia pestis, using three different expression systems

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3598636

Positive-selection vectors using the F plasmid ccdB killer gene

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3598636

Up-promoter mutations in the lpp gene of Escherichia coli

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3598636

High-throughput protein expression screening and purification in Escherichia coli.

7 July 2018

1 reference

https://api.crossref.org/works/10.1186%2F1472-6750-13-12

Applications of the Restriction Free (RF) cloning procedure for molecular manipulations and protein expression.

21 January 2018

1 reference

https://api.crossref.org/works/10.1186%2F1472-6750-13-12

Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC.

21 January 2018

1 reference

https://api.crossref.org/works/10.1186%2F1472-6750-13-12

Analysis of actin assembly by in vitro TIRF microscopy.

21 January 2018

1 reference

https://api.crossref.org/works/10.1186%2F1472-6750-13-12

SLIC: a method for sequence- and ligation-independent cloning.

21 January 2018

1 reference

https://api.crossref.org/works/10.1186%2F1472-6750-13-12

21 January 2018

Identifiers

DOI

10.1186/1472-6750-13-12

1 reference

Dimensions Publication ID

3 August 2017

0 references

1 reference

3 August 2017

1 reference