(Q35092096)

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One in a million: flow cytometric sorting of single cell-lysate assays in monodisperse picolitre double emulsion droplets for directed evolution

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scholarly article

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Europe PubMed Central

6 August 2017

One in a million: flow cytometric sorting of single cell-lysate assays in monodisperse picolitre double emulsion droplets for directed evolution (English)

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Europe PubMed Central

6 August 2017

directed evolution

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based on heuristic

inferred from title

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5 June 2020

Martin Fischlechner

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5 June 2020

Florian Hollfelder

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5 June 2020

Anastasia Zinchenko

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5 June 2020

Pierre-Yves Colin

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5 June 2020

author name string

Sean R A Devenish

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5 June 2020

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11 February 2014

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Analytical Chemistry

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5 June 2020

86

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5 June 2020

2526-2533

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5 June 2020

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5 June 2020

Ultrahigh-throughput sorting of microfluidic drops with flow cytometry

1 reference

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8 July 2018

A fully unsupervised compartment-on-demand platform for precise nanoliter assays of time-dependent steady-state enzyme kinetics and inhibition.

1 reference

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8 July 2018

A programmable microenvironment for cellular studies via microfluidics-generated double emulsions.

1 reference

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8 July 2018

Efficient, crosswise catalytic promiscuity among enzymes that catalyze phosphoryl transfer

1 reference

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8 July 2018

Vanadium bromoperoxidase-coupled fluorescent assay for flow cytometry sorting of glucose oxidase gene libraries in double emulsions

1 reference

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8 July 2018

Engineering the third wave of biocatalysis

1 reference

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8 July 2018

Droplet microfluidics for high-throughput biological assays

1 reference

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8 July 2018

Microfluidic droplets: new integrated workflows for biological experiments

1 reference

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8 July 2018

Microfluidic compartmentalized directed evolution

1 reference

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8 July 2018

Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology

1 reference

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8 July 2018

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

1 reference

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8 July 2018

The potential of microfluidic water-in-oil droplets in experimental biology

1 reference

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8 July 2018

Ultrahigh-throughput FACS-based screening for directed enzyme evolution

1 reference

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8 July 2018

Directed evolution drives the next generation of biocatalysts

1 reference

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8 July 2018

Efficient catalytic promiscuity in an enzyme superfamily: an arylsulfatase shows a rate acceleration of 10(13) for phosphate monoester hydrolysis

1 reference

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8 July 2018

High-throughput quantitative polymerase chain reaction in picoliter droplets

1 reference

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8 July 2018

Droplet-based microfluidic systems for high-throughput single DNA molecule isothermal amplification and analysis

1 reference

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8 July 2018

Microdroplets: a sea of applications?

1 reference

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8 July 2018

Drop-based microfluidic devices for encapsulation of single cells

1 reference

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8 July 2018

Sizing subcellular organelles and nanoparticles confined within aqueous droplets

1 reference

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High-throughput screening methodology for the directed evolution of glycosyltransferases

1 reference

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Miniaturising the laboratory in emulsion droplets

1 reference

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8 July 2018

High-throughput screening of enzyme libraries: in vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions.

1 reference

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8 July 2018

High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3952496

8 July 2018

Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization

1 reference

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8 July 2018

In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting

1 reference

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8 July 2018

Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization

1 reference

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1 reference

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8 July 2018

1.3 A structure of arylsulfatase from Pseudomonas aeruginosa establishes the catalytic mechanism of sulfate ester cleavage in the sulfatase family

1 reference

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8 July 2018

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1 reference

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1 reference

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27 September 2018

Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution.

1 reference

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1 reference

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1 reference

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1 reference

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1 reference

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Multi-step microfluidic droplet processing: kinetic analysis of an in vitro translated enzyme.

1 reference

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1 reference

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1 reference

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27 September 2018

Monodisperse double emulsions generated from a microcapillary device.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3952496

27 September 2018

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1 reference

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27 September 2018

Evolution in microfluidic droplet

1 reference

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Directed evolution of hydrolases for prevention of G-type nerve agent intoxication

1 reference

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27 September 2018

Directed evolution by in vitro compartmentalization

1 reference

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Mapping catalytic promiscuity in the alkaline phosphatase superfamily

1 reference

20 August 2023

https://opencitations.net/index/coci/api/v1/citations/10.1351/PAC-CON-08-10-20

Identifiers

10.1021/AC403585P

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Europe PubMed Central

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5 June 2020

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Europe PubMed Central

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5 June 2020

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Europe PubMed Central

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5 June 2020

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