(Q35740372)

10 August 2017

title

Dissecting enzyme function with microfluidic-based deep mutational scanning (English)

1 reference

10 August 2017

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deep mutational scanning

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author name string

Philip A Romero

series ordinal

1

1 reference

10 August 2017

Tuan M Tran

series ordinal

2

1 reference

10 August 2017

Adam R Abate

series ordinal

3

1 reference

10 August 2017

language of work or name

English

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publication date

26 May 2015

1 reference

Proceedings of the National Academy of Sciences of the United States of America

10 August 2017

number of pages

6

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based on heuristic

inferred from page(s)

published in

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10 August 2017

volume

112

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10 August 2017

issue

23

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10 August 2017

page(s)

7159-7164

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Using droplet-based microfluidics to improve the catalytic properties of RNA under multiple-turnover conditions

10 August 2017

cites work

1 reference

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Deep mutational scanning: a new style of protein science

12 July 2018

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CotA laccase: high-throughput manipulation and analysis of recombinant enzyme libraries expressed in E. coli using droplet-based microfluidics

12 July 2018

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12 July 2018

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12 July 2018

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12 July 2018

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12 July 2018

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Capturing the mutational landscape of the beta-lactamase TEM-1.

12 July 2018

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12 July 2018

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12 July 2018

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Systematic identification of H274Y compensatory mutations in influenza A virus neuraminidase by high-throughput screening

12 July 2018

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12 July 2018

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A fundamental protein property, thermodynamic stability, revealed solely from large-scale measurements of protein function

12 July 2018

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12 July 2018

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Stabilizing proteins from sequence statistics: the interplay of conservation and correlation in triosephosphate isomerase stability

12 July 2018

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Fast gapped-read alignment with Bowtie 2

12 July 2018

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Protein model discrimination using mutational sensitivity derived from deep sequencing

12 July 2018

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Experimental illumination of a fitness landscape

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4466731

A flow cytometry-based screening system for directed evolution of proteases.

12 July 2018

1 reference

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Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries

12 July 2018

1 reference

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Exploring challenges in rational enzyme design by simulating the catalysis in artificial kemp eliminase

12 July 2018

1 reference

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An exciting but challenging road ahead for computational enzyme design

12 July 2018

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High-resolution mapping of protein sequence-function relationships

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4466731

High-throughput screening of enzymes by retroviral display using droplet-based microfluidics

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4466731

Origins of catalysis by computationally designed retroaldolase enzymes

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4466731

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4466731

Parallel, tag-directed assembly of locally derived short sequence reads

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4466731

Protein sectors: evolutionary units of three-dimensional structure

12 July 2018

1 reference

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Phylogeny.fr: robust phylogenetic analysis for the non-specialist

12 July 2018

1 reference

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Evolution favors protein mutational robustness in sufficiently large populations

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4466731

Robustness-epistasis link shapes the fitness landscape of a randomly drifting protein.

12 July 2018

1 reference

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Molecular basis of substrate specificity in family 1 glycoside hydrolases

12 July 2018

1 reference

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High-throughput screens and selections of enzyme-encoding genes.

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4466731

Thermodynamic prediction of protein neutrality

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4466731

Protein tolerance to random amino acid change

12 July 2018

1 reference

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MUSCLE: multiple sequence alignment with high accuracy and high throughput

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4466731

The depth of chemical time and the power of enzymes as catalysts.

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4466731

Directed evolution of polymerase function by compartmentalized self-replication

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4466731

Structures and mechanisms of glycosyl hydrolases

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4466731

Picoliter cell lysate assays in microfluidic droplet compartments for directed enzyme evolution.

12 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4466731

A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution.

25 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4466731

From microdroplets to microfluidics: selective emulsion separation in microfluidic devices

25 September 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4466731

Glycosidase mechanisms: anatomy of a finely tuned catalyst

25 September 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/26040002

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1073/PNAS.1422285112

1 reference

10 August 2017

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1 reference

10 August 2017

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