(Q35789246)

10 August 2017

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title

PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings (English)

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10 August 2017

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Richard E Rothman

2

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author name string

Samuel Yang

series ordinal

1

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10 August 2017

1 June 2004

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Lancet Infectious Diseases

10 August 2017

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10 August 2017

4

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10 August 2017

337-348

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10 August 2017

6

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Rapid diagnosis of pulmonary tuberculosis by using Roche AMPLICOR Mycobacterium tuberculosis PCR test.

10 August 2017

1 reference

12 December 2020

Amplification of bacterial DNA using highly conserved sequences: automated analysis and potential for molecular triage of sepsis.

1 reference

12 December 2020

Diagnostic analyses of biological agent-caused syndromes: laboratory and technical assistance

1 reference

12 December 2020

Outbreak of severe acute respiratory syndrome--worldwide, 2003.

1 reference

12 December 2020

Development of broad-range polymerase chain reaction (PCR) bacterial identification in diagnosis of infective endocarditis.

1 reference

12 December 2020

Evaluation of affirm VP Microbial Identification Test for Gardnerella vaginalis and Trichomonas vaginalis

1 reference

12 December 2020

Molecular typing of enteroviruses: current status and future requirements. The European Union Concerted Action on Virus Meningitis and Encephalitis

1 reference

12 December 2020

Does blood of healthy subjects contain bacterial ribosomal DNA?

1 reference

12 December 2020

Detection of vaccinia virus, herpes simplex virus, varicella-zoster virus, and Bacillus anthracis DNA by LightCycler polymerase chain reaction after autoclaving: implications for biosafety of bioterrorism agents

1 reference

12 December 2020

Comparative evaluation of two commercial amplification assays for direct detection of Mycobacterium tuberculosis complex in respiratory specimens.

1 reference

12 December 2020

Detection of Francisella tularensis within infected mouse tissues by using a hand-held PCR thermocycler

1 reference

12 December 2020

Detection and identification of fungi in blood using broad-range 28S rDNA PCR amplification and species-specific hybridisation.

1 reference

12 December 2020

Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in suspected cases of meningitis and septicemia using real-time PCR.

1 reference

12 December 2020

Methods for improved detection of oxacillin resistance in coagulase-negative staphylococci: results of a multicenter study.

1 reference

12 December 2020

Ability of the digene hybrid capture II test to identify Chlamydia trachomatis and Neisseria gonorrhoeae in cervical specimens

1 reference

12 December 2020

Hospitalization vs outpatient treatment of young febrile infants: 10-year comparison

1 reference

12 December 2020

Identification of nucleotide sequences for the specific and rapid detection of Yersinia pestis.

1 reference

12 December 2020

Detection of Chlamydia trachomatis antigens by enzyme immunoassay and immunofluorescence in genital specimens from symptomatic and asymptomatic men and women

1 reference

12 December 2020

Determination of hepatitis B virus DNA in serum using the polymerase chain reaction: clinical significance and correlation with serological and biochemical markers

1 reference

12 December 2020

Expenditure and loss of income incurred by tuberculosis patients before reaching effective treatment in Bangladesh

1 reference

12 December 2020

Interaction of Chlamydia pneumoniae infection with other risk factors of atherosclerosis

1 reference

12 December 2020

Molecular diagnostics for existing and emerging infections. Complementary tools for a new era of clinical microbiology.

1 reference

12 December 2020

The impact of an enteroviral RT-PCR assay on the diagnosis of aseptic meningitis and patient management

1 reference

12 December 2020

Molecular beacons: probes that fluoresce upon hybridization

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12 December 2020

The impact of the polymerase chain reaction in clinical medicine

1 reference

12 December 2020

Detection by polymerase chain reaction of Treponema pallidum DNA in cerebrospinal fluid from neurosyphilis patients before and after antibiotic treatment

1 reference

12 December 2020

Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction.

1 reference

12 December 2020

Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions

1 reference

12 December 2020

Molecular approaches to detecting herpes simplex virus and enteroviruses in the central nervous system

1 reference

12 December 2020

Accurate mass measurement of DNA oligonucleotide ions using high-resolution time-of-flight mass spectrometry

1 reference

12 December 2020

Broad range DNA probes for detecting and amplifying eubacterial nucleic acids.

1 reference

12 December 2020

Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification

1 reference

12 December 2020

Detection and quantification of virus DNA in plasma of patients with Epstein-Barr virus-associated diseases.

1 reference

12 December 2020

Point-of-care testing: an introduction

1 reference

12 December 2020

Detection of Bacillus anthracis DNA by LightCycler PCR.

1 reference

12 December 2020

Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons

1 reference

12 December 2020

Unsuspected gonorrhea and chlamydia in patients of an urban adult emergency department: a critical population for STD control intervention.

1 reference

12 December 2020

RAPD typing in microbiology--a technical review

1 reference

12 December 2020

Recent advances in the polymerase chain reaction

1 reference

12 December 2020

Hospitalization decision in febrile intravenous drug users.

1 reference

12 December 2020

Sequence capture-PCR improves detection of mycobacterial DNA in clinical specimens

1 reference

12 December 2020

Elimination of bacterial DNA from Taq DNA polymerases by restriction endonuclease digestion.

1 reference

12 December 2020

Inability to predict diagnosis in febrile intravenous drug abusers

1 reference

12 December 2020

Detection of enterotoxigenic Escherichia coli by DNA colony hybridization

1 reference

12 December 2020

Molecular diagnosis of bacterial endocarditis by broad-range PCR amplification and direct sequencing

1 reference

12 December 2020

Identification of the uncultured bacillus of Whipple's disease

1 reference

12 December 2020

The role of DNA amplification technology in the diagnosis of infectious diseases

1 reference

12 December 2020

Perinatal screening for group B streptococci: cost-benefit analysis of rapid polymerase chain reaction

1 reference

12 December 2020

Identification of a novel coronavirus in patients with severe acute respiratory syndrome

1 reference

12 December 2020

Detection of bacteraemia in patients with fever and neutropenia using 16S rRNA gene amplification by polymerase chain reaction.

1 reference

12 December 2020

The agent of bacillary angiomatosis. An approach to the identification of uncultured pathogens.

1 reference

12 December 2020

Evaluation of bias in diagnostic-test sensitivity and specificity estimates computed by discrepant analysis

1 reference

12 December 2020

Clinical aspects of diagnosis of gonorrhea and Chlamydia infection in an acute care setting

1 reference

12 December 2020

Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes

1 reference

12 December 2020

Quantification of viral load: clinical relevance for human immunodeficiency virus, hepatitis B virus and hepatitis C virus infection

1 reference

12 December 2020

Molecular methods for diagnosis of infective endocarditis

1 reference

12 December 2020

Principles of molecular microbiology testing methods

1 reference

12 December 2020

Quantitative multiprobe PCR assay for simultaneous detection and identification to species level of bacterial pathogens

1 reference

12 December 2020

Polymerase chain reaction for assessing treatment response in patients with pulmonary tuberculosis.

1 reference

12 December 2020

Genotypic assessment of isoniazid and rifampin resistance in Mycobacterium tuberculosis: a blind study at reference laboratory level.

1 reference

12 December 2020

Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification

1 reference

12 December 2020

Faster... but fast enough? Responding to the epidemic of severe acute respiratory syndrome.

1 reference

12 December 2020

Detection of viable Vibrio cholerae by reverse-transcriptase polymerase chain reaction (RT-PCR)

1 reference

12 December 2020

Diagnosis of Chlamydia trachomatis infections in men and women by testing first-void urine by ligase chain reaction.

1 reference

12 December 2020

Molecular pathology--diagnosis of infectious disease

1 reference

12 December 2020

The relation between baseline HIV drug resistance and response to antiretroviral therapy: re-analysis of retrospective and prospective studies using a standardized data analysis plan.

1 reference

12 December 2020

Clinical Evaluation of the Gen-Probe amplified mycobacterium tuberculosis direct test for rapid detection of Mycobacterium tuberculosis in select nonrespiratory specimens

1 reference

12 December 2020

Will cultures survive? The role of molecular tests in diagnostic bacteriology

1 reference

12 December 2020

Molecular bacteriology: a diagnostic tool for the millennium

1 reference

12 December 2020

The impact of antimicrobial resistance on health and economic outcomes

1 reference

12 December 2020

DNA chips: state-of-the art.

1 reference

12 December 2020

Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications.

1 reference

12 December 2020

Clinical use of genotypic and phenotypic drug resistance testing to monitor antiretroviral chemotherapy

1 reference

12 December 2020

Contamination and sensitivity issues with a real-time universal 16S rRNA PCR

1 reference

12 December 2020

Epidemiology of sepsis syndrome in 8 academic medical centers

1 reference

12 December 2020

The unusual origin of the polymerase chain reaction

1 reference

12 December 2020

Evaluation of the Roche AMPLICOR enterovirus PCR assay in the diagnosis of enteroviral central nervous system infections

1 reference

12 December 2020

Bioterrorism: implications for the clinical microbiologist

1 reference

12 December 2020

Direct detection of Chlamydia trachomatis in urine specimens from symptomatic and asymptomatic men by using a rapid polymerase chain reaction assay

1 reference

12 December 2020

Human papillomavirus testing in primary screening for the detection of high-grade cervical lesions: a study of 7932 women

1 reference

12 December 2020

Analytical performance and clinical utility of a nucleic acid sequence-based amplification assay for detection of cytomegalovirus infection.

1 reference

12 December 2020

Diagnosis of Chlamydia trachomatis genitourinary infection in women by ligase chain reaction assay of urine

1 reference

12 December 2020

Fluorescence energy transfer detection as a homogeneous DNA diagnostic method

1 reference

12 December 2020

Multiplex PCR: optimization and application in diagnostic virology

1 reference

12 December 2020

Head-to-head multicenter comparison of DNA probe and nucleic acid amplification tests for Chlamydia trachomatis infection in women performed with an improved reference standard

1 reference

12 December 2020

Time-of-flight mass spectrometry for the structural analysis of biological molecules

1 reference

12 December 2020

Quantitative analysis of mRNA as a marker for viability of Mycobacterium tuberculosis

1 reference

12 December 2020

Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells.

1 reference

12 December 2020

Successful testing protocols in virology.

1 reference

12 December 2020

Single-tube, nested, reverse transcriptase PCR for detection of viable Mycobacterium tuberculosis

1 reference

12 December 2020

Detection of microbial nucleic acids for diagnostic purposes

1 reference

12 December 2020

Determination of Mycobacterium leprae viability by polymerase chain reaction amplification of 71-kDa heat-shock protein mRNA

1 reference

12 December 2020

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS).

1 reference

12 December 2020

Quantitation of HIV-1 RNA in plasma predicts outcome after seroconversion

1 reference

12 December 2020

[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

1 reference

12 December 2020

A novel repeated DNA sequence located in the intergenic regions of bacterial chromosomes.

1 reference

12 December 2020

Multicenter Evaluation of the VERSANT HCV RNA Qualitative Assay for Detection of Hepatitis C Virus RNA

1 reference

12 December 2020

A comparison of nucleic acid amplification techniques for the assessment of bacterial viability

1 reference

12 December 2020

PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid.

1 reference

12 December 2020

Multicenter evaluation of the Bayer VERSANT HIV-1 RNA 3.0 assay: analytical and clinical performance

1 reference

12 December 2020

Real time quantitative PCR

1 reference

12 December 2020

Microfabrication technologies for integrated nucleic acid analysis

1 reference

12 December 2020

Quality control PCR: a method for detecting inhibitors of Taq DNA polymerase.

1 reference

12 December 2020

Evaluation of COBAS AMPLICOR (Roche): accuracy in detection of Chlamydia trachomatis and Neisseria gonorrhoeae by coamplification of endocervical specimens

1 reference

12 December 2020

Molecular biology for the critical care physician. Part II: where are we now?

1 reference

12 December 2020

Meningococcal bacterial DNA load at presentation correlates with disease severity

1 reference

12 December 2020

Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS).

1 reference

12 December 2020

Discrepant analysis: how can we test a test?

1 reference

12 December 2020

Base-specific fragmentation of amplified 16S rRNA genes analyzed by mass spectrometry: a tool for rapid bacterial identification

1 reference

12 December 2020

The polymerase chain reaction. A new method of using molecular genetics for medical diagnosis

1 reference

12 December 2020

Diagnostic molecular microbiology. Current challenges and future directions

1 reference

12 December 2020

Identification of mycobacteria from culture by using the Gen-Probe Rapid Diagnostic System for Mycobacterium avium complex and Mycobacterium tuberculosis complex

1 reference

12 December 2020

Viral kinetics can predict early response to alpha-interferon in chronic hepatitis C.

1 reference

12 December 2020

Quantitation of human immunodeficiency virus type 1 in the blood of infected persons

1 reference

12 December 2020

Antimicrobial resistance and bacterial identification utilizing a microelectronic chip array

1 reference

12 December 2020

Simultaneous amplification and detection of specific DNA sequences

1 reference

12 December 2020

MALDI-TOF analysis of polymerase chain reaction products from methanotrophic bacteria

1 reference

12 December 2020

Clinical specificity and sensitivity of a blood screening assay for detection of HIV-1 and HCV RNA.

1 reference

12 December 2020

PCR strategy for identification and differentiation of small pox and other orthopoxviruses.

1 reference

12 December 2020

Microarray technology: the future of blood testing?

1 reference

12 December 2020

Exploring the new world of the genome with DNA microarrays

1 reference

12 December 2020

Detection of Bacteremia in Emergency Department Patients at Risk for Infective Endocarditis Using Universal 16S rRNA Primers in a Decontaminated Polymerase Chain Reaction Assay

1 reference

12 December 2020

Evaluation of the digene hybrid capture II CT-ID test for detection of Chlamydia trachomatis in endocervical specimens.

1 reference

12 December 2020

The detection of microbial DNA in the blood: a sensitive method for diagnosing bacteremia and/or bacterial translocation in surgical patients

1 reference

12 December 2020

Evaluation of the VERSANT HCV RNA 3.0 assay for quantification of hepatitis C virus RNA in serum

1 reference

12 December 2020

Direct DNA probe assay for Neisseria gonorrhoeae in pharyngeal and rectal specimens

1 reference

12 December 2020

Discrepant analysis: a biased and an unscientific method for estimating test sensitivity and specificity

1 reference

12 December 2020

Multicenter comparison of the digene hybrid capture CMV DNA assay (version 2.0), the pp65 antigenemia assay, and cell culture for detection of cytomegalovirus viremia.

1 reference

12 December 2020

Tech.Sight. Molecular testing for infectious disease

1 reference

12 December 2020

Increase in National Hospital Discharge Survey rates for septicemia--United States, 1979-1987.

1 reference

12 December 2020

Molecular diagnostics of infectious diseases.

1 reference

12 December 2020

Rapid identification of Mycobacterium avium complex in culture using DNA probes.

1 reference

12 December 2020

Control of mRNA processing and decay in prokaryotes

1 reference

12 December 2020

The polymerase chain reaction: clinical applications

1 reference

12 December 2020

Real-time PCR in virology

1 reference

12 December 2020

Line probe assay in the rapid detection of rifampin-resistant Mycobacterium tuberculosis directly from clinical specimens.

1 reference

12 December 2020

Rapid detection of rifampicin resistance in sputum and biopsy specimens from tuberculosis patients by PCR and line probe assay.

1 reference

12 December 2020

International perspectives on antiretroviral resistance. Phenotypic and genotypic resistance assays: methodology, reliability, and interpretations

1 reference

12 December 2020

Diagnosis of enteroviral meningitis with the polymerase chain reaction

1 reference

12 December 2020

Coronavirus as a possible cause of severe acute respiratory syndrome

1 reference

12 December 2020

Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by enzyme immunoassay, culture, and three nucleic acid amplification tests

1 reference

12 December 2020

More keys to padlock probes: mechanisms for high-throughput nucleic acid analysis

1 reference

12 December 2020

Clinical evaluation of the enhanced Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test for rapid diagnosis of tuberculosis in prison inmates

1 reference

12 December 2020

Current methods of laboratory diagnosis of Chlamydia trachomatis infections

1 reference

12 December 2020

Application of polymerase chain reaction to the diagnosis of infectious diseases.

1 reference

12 December 2020

The Unusual Origin of the Polymerase Chain Reaction

1 reference

Crossref

https://api.crossref.org/works/10.1016%2FS1473-3099%2804%2901044-8

7 January 2021

inferred from DOI database lookup

Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

1 reference

12 December 2020

10.1016/S1473-3099(04)01044-8

Identifiers

DOI

1 reference

10 August 2017

1 reference

PubMed ID

15172342